Catalog No.
YVV14704
Expression system
E. coli
Species
Bovine viral diarrhea virus (isolate NADL) (BVDV) (Mucosal disease virus)
Protein length
Met1537-Pro1617
Predicted molecular weight
37.55 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P19711
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Genome polyprotein, Cysteine protease NS2, 3.4.22.-, Non-structural protein 2, p54
Packaging defects in pestiviral NS4A can be compensated by mutations in NS2 and NS3., PMID:37695056
Non-structural proteins of bovine viral diarrhea virus., PMID:35614328
A novel NS3/4A protease dependent cleavage site within pestiviral NS2., PMID:34676824
Characterization of a Cytopathogenic Reporter CSFV., PMID:34201706
The identification of a B-cell epitope in bovine viral diarrhea virus (BVDV) core protein based on a mimotope obtained from a phage-displayed peptide library., PMID:34111485
Novel Potent IFN-γ-Inducing CD8+ T Cell Epitopes Conserved among Diverse Bovine Viral Diarrhea Virus Strains., PMID:33762324
Membrane Topology of Pestiviral Non-Structural Protein 2 and determination of the minimal autoprotease domain., PMID:33731461
Mosaic Bovine Viral Diarrhea Virus Antigens Elicit Cross-Protective Immunity in Calves., PMID:33281819
Genomic and antigenic characterization of a cytopathic bovine viral diarrhea virus 1i isolated in the United States., PMID:31357167
Determination of Critical Requirements for Classical Swine Fever Virus NS2-3-Independent Virion Formation., PMID:31292243
CRISPR/Cas9-Mediated Knockout of DNAJC14 Verifies This Chaperone as a Pivotal Host Factor for RNA Replication of Pestiviruses., PMID:30518653
Pig BVDV-2 non-structural protein (Npro) links to cellular antiviral response in vitro., PMID:27866318
Characterization of the Determinants of NS2-3-Independent Virion Morphogenesis of Pestiviruses., PMID:26355097
Characterization of the cytopathic BVDV strains isolated from 13 mucosal disease cases arising in a cattle herd., PMID:25300803
Stability of the resistance to the thiosemicarbazone derived from 5,6-dimethoxy-1-indanone, a non-nucleoside polymerase inhibitor of bovine viral diarrhea virus., PMID:24950191
Mixed triple: allied viruses in unique recent isolates of highly virulent type 2 bovine viral diarrhea virus detected by deep sequencing., PMID:24719408
Immunology of BVDV vaccines., PMID:22883306
Antigenic relationships between Bovine viral diarrhea virus 1 and 2 and HoBi virus: possible impacts on diagnosis and control., PMID:22379042
Pestivirus virion morphogenesis in the absence of uncleaved nonstructural protein 2-3., PMID:22031952
Identification and characterization of the host protein DNAJC14 as a broadly active flavivirus replication modulator., PMID:21249176
Genetic and antigenic characterization of bovine viral diarrhoea virus type 2 isolated from cattle in India., PMID:21112633
Genetic and antigenic typing of border disease virus (BDV) isolates from Italy reveals the existence of a novel BDV group., PMID:20656426
Complete genomic sequence of a border disease virus isolated from Pyrenean chamois., PMID:20638945
The extra 16-amino-acid peptide at C-terminal NS2 of the hypervirulent type-2 bovine viral diarrhea viruses has no effect on viral replication and NS2-3 processing of type-1 virus., PMID:20589424
Molecular characterization of RNA and protein synthesis during a one-step growth curve of bovine viral diarrhoea virus in ovine (SFT-R) cells., PMID:20116078
Expression of the NS3 protease of cytopathogenic bovine viral diarrhea virus results in the induction of apoptosis but does not block activation of the beta interferon promoter., PMID:19793904
A single point mutation in nonstructural protein NS2 of bovine viral diarrhea virus results in temperature-sensitive attenuation of viral cytopathogenicity., PMID:19776121
Antigenic differentiation of classical swine fever viruses in China by monoclonal antibodies., PMID:19428750
A replicon trans-packaging system reveals the requirement of nonstructural proteins for the assembly of bovine viral diarrhea virus (BVDV) virion., PMID:19327808
Inhibition of sphingosine kinase by bovine viral diarrhea virus NS3 is crucial for efficient viral replication and cytopathogenesis., PMID:19293152
Cytopathogenicity of classical Swine Fever virus correlates with attenuation in the natural host., PMID:18653456
Cleavage of the NS2-3 protein in the cells of cattle persistently infected with non-cytopathogenic bovine viral diarrhea virus., PMID:18557898
Development and application of an enzyme-linked immunosorbent assay for detection of bovine viral diarrhea antibody based on Erns glycoprotein expressed in a baculovirus system., PMID:17459828
A search for RNA insertions and NS3 gene duplication in the genome of cytopathic isolates of bovine viral diarrhea virus., PMID:16862285
Lymphocytopathogenic activity in vitro correlates with high virulence in vivo for BVDV type 2 strains: Criteria for a third biotype of BVDV., PMID:16384618
A 45-nucleotide insertion in the NS2 gene is responsible for the cytopathogenicity of a bovine viral diarrhoea virus strain., PMID:16025238
Viral sequence insertions and a novel cellular insertion in the NS2 gene of cytopathic isolates of bovine viral diarrhea virus as potential cytopathogenicity markers., PMID:15744562
Temporal modulation of an autoprotease is crucial for replication and pathogenicity of an RNA virus., PMID:15367643
Uncleaved NS2-3 is required for production of infectious bovine viral diarrhea virus., PMID:14963137
Differences in membrane association and sub-cellular distribution between NS2-3 and NS3 of bovine viral diarrhoea virus., PMID:14602200
Cell-derived sequences in the N-terminal region of the polyprotein of a cytopathogenic pestivirus., PMID:12970452
Cytopathogenicity of pestiviruses: cleavage of bovine viral diarrhea virus NS2-3 has to occur at a defined position to allow viral replication., PMID:12827468
Informative value of an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of bovine viral diarrhoea virus (BVDV) antibodies in milk., PMID:11765806
Localization of viral proteins in cells infected with bovine viral diarrhoea virus., PMID:11602770
Isolation and characterization of noncytopathic pestivirus mutants reveals a role for nonstructural protein NS4B in viral cytopathogenicity., PMID:11602707
Recombination with a cellular mRNA encoding a novel DnaJ protein results in biotype conversion in genotype 2 bovine viral diarrhea viruses., PMID:11551646
Evaluation of a blocking ELISA for the detection of bovine viral diarrhoea virus (BVDV) antibodies in serum and milk., PMID:11348769
Antigenic characterization of bovine viral diarrhoea virus isolates from Spain with a panel of monoclonal antibodies., PMID:11244871
Insertion of cellular NEDD8 coding sequences in a pestivirus., PMID:11118368
A universal 'one-tube' RT-PCR protocol for amplifying isolates of bovine viral diarrhoea virus., PMID:11085469
For research use only. Not for human or drug use.
