Catalog No.
EVV28401
Expression system
Insect Cells
Species
Classical swine fever virus (strain Brescia) (CSFV) (Hog cholera virus)
Protein length
Ser3181-Gly3897
Predicted molecular weight
83.25 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
AAT85717
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
polyprotein, polyprotein, NS5B, RNA-directed RNA polymerase, p75
PKM2 Facilitates Classical Swine Fever Virus Replication by Enhancing NS5B Polymerase Function., PMID:40431660
Evolutionary-Related High- and Low-Virulent Classical Swine Fever Virus Isolates Reveal Viral Determinants of Virulence., PMID:38275957
Proline to Threonine Mutation at Position 162 of NS5B of Classical Swine Fever Virus Vaccine C Strain Promoted Genome Replication and Infectious Virus Production by Facilitating Initiation of RNA Synthesis., PMID:34452387
Genotyping and Molecular Characterization of Classical Swine Fever Virus Isolated in China during 2016-2018., PMID:33921513
The ESCRT-I Subunit Tsg101 Plays Novel Dual Roles in Entry and Replication of Classical Swine Fever Virus., PMID:33328308
Classical Swine Fever Virus Infection Induces Endoplasmic Reticulum Stress-Mediated Autophagy to Sustain Viral Replication in vivo and in vitro., PMID:31798542
Determination of Critical Requirements for Classical Swine Fever Virus NS2-3-Independent Virion Formation., PMID:31292243
A unique intra-molecular fidelity-modulating mechanism identified in a viral RNA-dependent RNA polymerase., PMID:30239956
Crystal Structure of Classical Swine Fever Virus NS5B Reveals a Novel N-Terminal Domain., PMID:29720518
Porcine Mx1 Protein Inhibits Classical Swine Fever Virus Replication by Targeting Nonstructural Protein NS5B., PMID:29343573
Porcine Viperin protein inhibits the replication of classical swine fever virus (CSFV) in vitro., PMID:29061156
Sequence adaptations during growth of rescued classical swine fever viruses in cell culture and within infected pigs., PMID:27527774
[Research Progress in the Core Proteins of the Classical Swine Fever Virus]., PMID:26738299
Episodic adaptive diversification of classical swine fever virus RNA-dependent RNA polymerase NS5B., PMID:26485449
Emergence of 2.1. subgenotype of classical swine fever virus in pig population of India in 2011., PMID:25365116
The classic swine fever virus (CSFV) core protein can enhance de novo-initiated RNA synthesis by the CSFV polymerase NS5B., PMID:24824302
Substituted 2,6-bis(benzimidazol-2-yl)pyridines: a novel chemical class of pestivirus inhibitors that targets a hot spot for inhibition of pestivirus replication in the RNA-dependent RNA polymerase., PMID:24680957
Application of real-time quantitative polymerase chain reaction to monitoring infection of classic swine fever virus and determining optimal harvest time in large-scale production., PMID:24021314
Characterization of the swine U6 promoter for short hairpin RNA expression and its application to inhibition of virus replication., PMID:23916945
Analysis of classical swine fever virus RNA replication determinants using replicons., PMID:23580431
Intra-host variation structure of classical swine fever virus NS5B in relation to antiviral therapy., PMID:23511203
Classical swine fever virus NS5A regulates viral RNA replication through binding to NS5B and 3'UTR., PMID:22795973
Classical swine fever virus NS5A protein interacts with 3'-untranslated region and regulates viral RNA synthesis., PMID:22261205
Classical swine fever virus NS5B protein suppresses the inhibitory effect of NS5A on viral translation by binding to NS5A., PMID:22258858
In vitro inhibition of CSFV replication by multiple siRNA expression., PMID:21699919
Biosynthesis of classical swine fever virus nonstructural proteins., PMID:21270154
Phylogenetic analysis of NS5B gene of classical swine fever virus isolates indicates plausible Chinese origin of Indian subgroup 2.2 viruses., PMID:21246270
Characterisation of interaction between NS3 and NS5B protein of classical swine fever virus by deletion of terminal sequences of NS5B., PMID:21237222
Classical swine fever virus NS3 is an IRES-binding protein and increases IRES-dependent translation., PMID:20637813
Validation of a loop-mediated isothermal amplification assay for visualised detection of wild-type classical swine fever virus., PMID:20304010
Classical swine fever virus NS3 enhances RNA-dependent RNA polymerase activity by binding to NS5B., PMID:19951725
Phylogenetic analysis of recent classical swine fever virus (CSFV) isolates from Assam, India., PMID:19896713
Characterization of NS3, NS5A and NS5B of classical swine fever virus through mutation and complementation analysis., PMID:19713054
Characterization of classical swine fever virus (CSFV) nonstructural protein 3 (NS3) helicase activity and its modulation by CSFV RNA-dependent RNA polymerase., PMID:19185595
The GTP binding sites interacted with RNA-dependent RNA polymerase of classical swine fever virus in de novo initiation., PMID:18430987
Effect of NS3 and NS5B proteins on classical swine fever virus internal ribosome entry site-mediated translation and its host cellular translation., PMID:18343841
In vitro inhibition of classical swine fever virus replication by siRNAs targeting Npro and NS5B genes., PMID:18262291
Genome comparison of a novel classical swine fever virus isolated in China in 2004 with other CSFV strains., PMID:16972026
Mutational analysis of the GDD sequence motif of classical swine fever virus RNA-dependent RNA polymerases., PMID:16917742
De novo RNA synthesis and homology modeling of the classical swine fever virus RNA polymerase., PMID:16022897
Origin and evolution of viruses causing classical swine fever in Cuba., PMID:15878213
Specific interaction between the classical swine fever virus NS5B protein and the viral genome., PMID:15373834
RNA-dependent RNA polymerase activity of Classical swine fever virus NS5B protein expressed in natural host cells., PMID:14524473
Characterization of RNA-dependent RNA polymerase activity of CSFV NS5B proteins expressed in Escherichia coli., PMID:12913359
Classical swine fever virus NS5B-GFP fusion protein possesses an RNA-dependent RNA polymerase activity., PMID:12209316
Genetic variation in the 5' end and NS5B regions of classical swine fever virus genome among Japanese isolates., PMID:10385204
Classical swine fever virus diversity and evolution., PMID:8683221
For research use only. Not for human or drug use.
