Catalog No.
YHB85301
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Met1-Asp187
Predicted molecular weight
22.97 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P00374
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Dihydrofolate reductase, DHFR
Pharmacogenetic Profiling of Genes Associated with Outcomes of Chemotherapy in Omani Healthy Controls., PMID:40428413
ITGA4 Contributes to 5-Fluorouracil Resistance by Up-Regulating PI3K/AKT Signaling: Evidence from Network Pharmacology, Molecular Docking and Experimental Verification., PMID:40416795
Identifying prognostic targets in metastatic prostate cancer beyond AR., PMID:40405591
Design, synthesis and in silico molecular modelling studies of 2-Hydrazineyl-2-oxoethyl-4-(1H-pyrrol-1-yb) benzoate derivatives: a potent dual DHFR and ENR-reductase inhibitors with antitubercular, antibacterial and cytotoxic potential., PMID:40388480
Discovery and optimization of 2-pyridones as dual h-DHFR/EGFRTK inhibitors with immunomodulatory potential; design, synthesis, anti-proliferative activity, and apoptosis inducer., PMID:40378576
De Novo Design of Highly Stable Binders Targeting Dihydrofolate Reductase in Klebsiella pneumoniae., PMID:40371895
Synthesis, characterization and bio-evaluation of novel series of pyrazoline derivatives as potential antifungal agents., PMID:40295598
Hastened Fusion-Dependent Endosomal Escape Improves Activity of Delivered Enzyme Cargo., PMID:40290140
Identification of novel long non-coding RNA involved in Sertoli cell of non-obstructive azoospermia based on microarray and bioinformatics analysis., PMID:40280502
Disparate co-evolution and prevalence of sulfadoxine and pyrimethamine resistance alleles and haplotypes at dhfr and dhps genes across Africa., PMID:40247054
Ultrasound-Assisted Synthesis of Substituted Chalcone-Linked 1,2,3-Triazole Derivatives as Antiproliferative Agents: In Vitro Antitumor Activity and Molecular Docking Studies., PMID:40244239
Prevalence of antimalaria drug resistance-conferring mutations associated with sulphadoxine-pyrimethamineine-resistant Plasmodium falciparum in East Africa: a systematic review and meta-analysis., PMID:40241183
Alleviation of accelerated diabetic atherogenesis in STZ-treated apoE/NOX1 DKO mice, apoE-/-/tg-EC-DHFR mice, and by folic acid., PMID:40184644
Novel 3-Substituted-2H-Chromene Scaffold Based Fluorinated Hydrophobic Fragment as In-Vitro Antiproliferative Agents and Apoptosis Inducers Targeting Both VEGFR-2/BRAFV600E and h-DHFR With Molecular Docking Simulation., PMID:40152211
Evaluating the Effect of Epigallocatechin Gallate (EGCG) in Reducing Folate Levels in Reproductive Aged Women by MTHFR and DHFR Genotype in Combination With Letrozole or Clomiphene., PMID:40077973
Development of a Dihydrofolate Reductase Selection System for Saccharomyces boulardii., PMID:40076696
Proteostasis modulates gene dosage evolution in antibiotic-resistant bacteria., PMID:40073078
Small extracellular vesicles and particles (sEVPs) derived from tumor-free pre-metastatic organs promote breast cancer metastasis and support organotropism., PMID:40057730
Super Methotrexate-resistant Osteosarcoma Cells Retain Their Sensitivity to Recombinant Methioninase: Targeting Methionine Addiction to Overcome Extreme Cancer-Chemotherapy Resistance., PMID:40037887
Molecular modeling of the interactions of compounds of Irvinga wombulu against dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum towards development of anti-malarial drug., PMID:39990732
DHFR2 RNA directly regulates dihydrofolate reductase and its expression level impacts folate one carbon metabolism., PMID:39957677
Crystal structure of the plasmid-encoded R67 dihydrofolate reductase complexed with Congo red an amyloid binding dye., PMID:39939735
Seesaw protein: Design of a protein that adopts interconvertible alternative functional conformations and its dynamics., PMID:39928865
Exploring Antibiotic Resistance in Diverse Homologs of the Dihydrofolate Reductase Protein Family through Broad Mutational Scanning., PMID:39896582
Quantifying protein unfolding kinetics with a high-throughput microfluidic platform., PMID:39868203
Resistance of Wolbachia to Trimethoprim: Insights into Genes Encoding Dihydrofolate Reductase, Thymidylate Synthase and Serine Hydroxymethyltransferase in the Rickettsiales., PMID:39859599
Monitoring molecular markers associated with antimalarial drug resistance in south-east Senegal from 2021 to 2023., PMID:39846779
Development of an attenuated glutamine synthetase (GS) selection system for the stable expression of tissue plasminogen activator in CHO-K1 cells., PMID:39838843
Structure and catalytic activity of a dihydrofolate reductase-like enzyme from Leptospira interrogans., PMID:39824401
Detection of low frequency artemisinin resistance mutations, C469Y, P553L and A675V, and fixed antifolate resistance mutations in asymptomatic primary school children in Kenya., PMID:39819451
Folic Acid and Methyltetrahydrofolate Supplementation in the Mthfr677C>T Mouse Model with Hepatic Steatosis., PMID:39796516
In defence of ferroptosis., PMID:39746918
Systematic characterization of indel variants using a yeast-based protein folding sensor., PMID:39706198
Molecular modeling, synthesis and biological evaluation of caffeic acid based Dihydrofolate reductase inhibitors., PMID:39696655
Doping dependency of chitosan and PAA controlled CdSe quantum dots for catalytic and bactericidal behavior by inhibiting DNA gyrase and DHFR through molecular docking., PMID:39672445
Recent Cutting-Edge Designing Strategies for Mtb-DHFR Inhibitors as Antitubercular Agents., PMID:39660864
Evaluation of 1-vinyl-3-alkyl imidazolium-based ionic liquid monomers towards antibacterial activity: An in-silico & in-vitro study., PMID:39642541
Synthesis, characterization, quantum chemical modelling, molecular docking, in silico and in vitro assessment of 3-(2-bromo-5-fluorophenyl))-1-(thiophen-2-yl)prop-2-en-1-one., PMID:39617796
Chaga Mushroom Triterpenoids Inhibit Dihydrofolate Reductase and Act Synergistically with Conventional Therapies in Breast Cancer., PMID:39595631
Trypanosoma cruzi infection in American black bears (Ursus americanus): A case report in a cub from California and serologic survey for exposure in wild black bears from several states., PMID:39550185
Prevalence of molecular markers of sulfadoxine-pyrimethamine resistance in Plasmodium falciparum isolates from West Africa during 2012-2022., PMID:39496624
Profile of molecular markers of Sulfadoxine-Pyrimethamine-resistant Plasmodium falciparum in individuals living in southern area of Brazzaville, Republic of Congo., PMID:39488015
Rational Exploration of 2,4-Diaminopyrimidines as DHFR Inhibitors Active against Mycobacterium abscessus and Mycobacterium avium, Two Emerging Human Pathogens., PMID:39468773
In-silico and in-vitro study of novel antimicrobial peptide AM1 from Aegle marmelos against drug-resistant Staphylococcus aureus., PMID:39468175
Evolution of Pfdhps and Pfdhfr mutations before and after adopting seasonal malaria chemoprevention in Nanoro, Burkina Faso., PMID:39414909
Molecular Dynamics Unveils Multiple-Site Binding of Inhibitors with Reduced Activity on the Surface of Dihydrofolate Reductase., PMID:39394997
Hastened fusion-dependent endosomal escape improves activity of delivered enzyme cargo., PMID:39386431
Statistical Coupling Analysis Predicts Correlated Motions in Dihydrofolate Reductase., PMID:39385339
Design, synthesis, in vitro and in silico studies of novel piperidine derived thiosemicarbazones as inhibitors of dihydrofolate reductase., PMID:39349528
Expression patterns of folate metabolism-related enzymes in the bovine oviduct: estrous cycle-dependent modulation and responsiveness to folic acid., PMID:39342825
For research use only. Not for human or drug use.
