Catalog No.
YHB04001
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Met1-Arg236
Predicted molecular weight
29.66 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
O60906
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Neutral sphingomyelinase, Lyso-PAF-PLC, SMPD2, Sphingomyelin phosphodiesterase 2, nSMase, Lyso-platelet-activating factor-phospholipase C, nSMase1, N-SMase
Exploring lipid metabolism-associated gene biomarkers and their regulatory mechanisms in nasopharyngeal carcinoma., PMID:40294966
Skin Protection Effects of Lactobacillus paragasseri HN910 Lysate and the Role of Alanine., PMID:40244537
The Ceramide-Dependent EV Secretome Differentially Affects Prostate Cancer Cell Migration., PMID:40214501
Modulation of Ceramide-Induced Apoptosis in Enteric Neurons by Aryl Hydrocarbon Receptor Signaling: Unveiling a New Pathway beyond ER Stress., PMID:39201268
Molecular basis for the catalytic mechanism of human neutral sphingomyelinases 1 (hSMPD2)., PMID:38012235
Abnormal expression of sphingolipid-metabolizing enzymes in the heart of spontaneously hypertensive rat models., PMID:37949293
Investigation of enzalutamide, docetaxel, and cabazitaxel resistance in the castration resistant prostate cancer cell line C4 using genome-wide CRISPR/Cas9 screening., PMID:37270558
The Effects and Mechanism of Scutellaria baicalensis Georgi Stems and Leaves Flavonoids on Myelin Sheath Degeneration Induced by Composite Aβ in Rats., PMID:37218194
Ectopic accumulation of ceramide in cardiomyocytes modulates alcoholic cardiomyopathy via the TLR4-dependent pathway., PMID:35373347
Genome-wide methylation association with current suicidal ideation in schizophrenia., PMID:32661777
Fingolimod Affects Transcription of Genes Encoding Enzymes of Ceramide Metabolism in Animal Model of Alzheimer's Disease., PMID:32356173
NADPH Oxidase-Mediated Activation of Neutral Sphingomyelinase Is Responsible for Diesel Particulate Extract-Induced Keratinocyte Apoptosis., PMID:32028642
Exosomal neutral sphingomyelinase 1 suppresses hepatocellular carcinoma via decreasing the ratio of sphingomyelin/ceramide., PMID:30106227
Decreased Serum Levels of Sphingomyelins and Ceramides in Sickle Cell Disease Patients., PMID:29663386
Neutral sphingomyelinases control extracellular vesicles budding from the plasma membrane., PMID:29184623
Trivalent chromium induces autophagy by activating sphingomyelin phosphodiesterase 2 and increasing cellular ceramide levels in renal HK2 cells., PMID:28577326
Ursodeoxycholic acid upregulates ERK and Akt in the protection of cardiomyocytes against CoCl2., PMID:27323195
CFTR and sphingolipids mediate hypoxic pulmonary vasoconstriction., PMID:25829545
Identifying rare variants for genetic risk through a combined pedigree and phenotype approach: application to suicide and asthma., PMID:25335167
Stress-induced ceramide generation and apoptosis via the phosphorylation and activation of nSMase1 by JNK signaling., PMID:25168245
Bioeffects of ultrasound-stimulated microbubbles on endothelial cells: gene expression changes associated with radiation enhancement in vitro., PMID:22980406
Targeting the ceramide system in cancer., PMID:21862212
Secretory mechanisms and intercellular transfer of microRNAs in living cells., PMID:20353945
Induction of membrane ceramides: a novel strategy to interfere with T lymphocyte cytoskeletal reorganisation in viral immunosuppression., PMID:19834551
Transcriptional regulation of neutral sphingomyelinase 2 gene expression of a human breast cancer cell line, MCF-7, induced by the anti-cancer drug, daunorubicin., PMID:19698806
Fas-mediated apoptosis is regulated by the extracellular matrix protein CCN1 (CYR61) in vitro and in vivo., PMID:19364818
Redox regulation of neutral sphingomyelinase-1 activity in HEK293 cells through a GSH-dependent mechanism., PMID:17169322
Modulation of Fas-induced apoptosis by p75 neurotrophin receptor in a human neuroblastoma cell line., PMID:16215672
Neutral sphingomyelinase 2 (smpd3) in the control of postnatal growth and development., PMID:15764706
Structural requirements for catalysis and membrane targeting of mammalian enzymes with neutral sphingomyelinase and lysophospholipid phospholipase C activities. Analysis by chemical modification and site-directed mutagenesis., PMID:10871611
For research use only. Not for human or drug use.
