Catalog No.
YXX23802
Expression system
E. coli
Species
Clostridium perfringens
Protein length
Ser31-Leu322
Predicted molecular weight
34.27 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
WP_110003253.1
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
necrotizing enteritis toxin NetB, netB
Oral and parenteral vaccination of broiler chickens with Recombinant NetB antigen from Clostridium perfringens confers significant protection against necrotic enteritis., PMID:40087742
Vaccination with formulations targeting Eimeria maxima and Clostridium perfringens conferred comprehensive protection using a dual-infection challenge model of necrotic enteritis., PMID:39708673
Elevated levels of butyric acid in the jejunum of an animal model of broiler chickens: from early onset of Clostridium perfringens infection to clinical disease of necrotic enteritis., PMID:39487547
Clostridium perfringens antigens and challenges for development of vaccines against necrotic enteritis in poultry., PMID:39187174
Supplementation with organic yeast-derived selenium provides immune protection against experimental necrotic enteritis in broiler chickens., PMID:38759933
Research Note: Clostridium perfringens NetB and CnaA neutralizing nanobodies in feed reduce the incidence of poultry necrotic enteritis., PMID:38417327
Exploring the predictive power of jejunal microbiome composition in clinical and subclinical necrotic enteritis caused by Clostridium perfringens: insights from a broiler chicken model., PMID:38243294
Hyperimmune egg yolk antibodies developed against Clostridium perfringens antigens protect against necrotic enteritis., PMID:37480657
Collagen adhesin protein and necrotic enteritis B-like toxin as biomarkers for early diagnosis of necrotic enteritis in commercial broiler chickens., PMID:37060834
Lactobacillus casei displaying Clostridium perfringens NetB antigen protects chickens against necrotic enteritis., PMID:36063180
Immunization with Pooled Antigens for Clostridium perfringens Conferred Partial Protection against Experimental Necrotic Enteritis in Broiler Chickens., PMID:35746587
Recombinant Limosilactobacillus (Lactobacillus) delivering nanobodies against Clostridium perfringens NetB and alpha toxin confers potential protection from necrotic enteritis., PMID:35478283
NanI sialidase contributes to toxin expression and host cell binding of Clostridium perfringens type G strain CP56 in vitro., PMID:35176607
Role of Clostridium perfringens Necrotic Enteritis B-like Toxin in Disease Pathogenesis., PMID:35062722
Experimental induction of necrotic enteritis with or without predisposing factors using netB positive Clostridium perfringens strains., PMID:34789342
Mesoporous silica nanoparticles-based formulations of a chimeric proteinous vaccine candidate against necrotic enteritis disease., PMID:34474867
Detection and Quantification of Clostridium perfringens and Eimeria spp. in Poultry Dust Using Real-Time PCR Under Experimental and Field Conditions., PMID:34339126
Comparative Genomics of Clostridium perfringens Reveals Patterns of Host-Associated Phylogenetic Clades and Virulence Factors., PMID:34177831
A bivalent vaccine against avian necrotic enteritis and coccidiosis., PMID:34101942
Evaluation of different antigenic preparations against necrotic enteritis in broiler birds using a novel Clostridium perfringens type G strain., PMID:33957249
Clostridium perfringens Produces an Adhesive Pilus Required for the Pathogenesis of Necrotic Enteritis in Poultry., PMID:33468589
Detection of Necrotic Enteritis B-like Toxin Secreted by Clostridium perfringens Using Capture Enzyme-Linked Immunosorbent Assay., PMID:33347555
The cytotoxicity and molecular mechanisms of the Clostridium perfringens NetB toxin., PMID:33342969
Correlation Between Clostridium perfringens Alpha- and NetB-Toxin and Chick Mortality in Commercial Broiler Farms During Different Anticoccidial Control Programs., PMID:33205177
Molecular Characterization of Clostridium perfringens Strains Isolated in Italy., PMID:33050097
Immunization with oral and parenteral subunit chimeric vaccine candidate confers protection against Necrotic Enteritis in chickens., PMID:33012608
Prevalence, toxin-typing, and antimicrobial susceptibility of Clostridium perfringens from retail meats in Seoul, Korea., PMID:32619505
Spotlight on avian pathology: untangling contradictory disease descriptions of necrotic enteritis and necro-haemorrhagic enteritis in broilers., PMID:32208870
Characterization of Virulent netB+/tpeL+Clostridium perfringens Strains from Necrotic Enteritis-Affected Broiler Chicken Farms., PMID:31967429
In silico design and in vitro analysis of a recombinant trivalent fusion protein candidate vaccine targeting virulence factor of Clostridium perfringens., PMID:31726124
Prevalence of netB-positive Clostridium perfringens in Italian poultry flocks by environmental sampling., PMID:31650911
The netB-positive Clostridium perfringens in the experimental induction of necrotic enteritis with or without predisposing factors., PMID:31222251
Evaluation of a toxoid fusion protein vaccine produced in plants to protect poultry against necrotic enteritis., PMID:30944775
Immunization with subunits of a novel pilus produced by virulent Clostridium perfringens strains confers partial protection against necrotic enteritis in chickens., PMID:30827407
Salmonella-vectored vaccine delivering three Clostridium perfringens antigens protects poultry against necrotic enteritis., PMID:30753181
In silico Identification of Novel Toxin Homologs and Associated Mobile Genetic Elements in Clostridium perfringens., PMID:30699957
Detection of chicken interleukin-10 production in intestinal epithelial cells and necrotic enteritis induced by Clostridium perfringens using capture ELISA., PMID:30596381
Characterization of intestinal immune response to Clostridium perfringens infection in broiler chickens., PMID:30239962
Characterization of toxin genes and quantitative analysis of netB in necrotic enteritis (NE)-producing and non-NE-producing Clostridium perfringens isolated from chickens., PMID:30170048
Characterization of Clostridium perfringens recovered from broiler chicken affected by necrotic enteritis., PMID:30053181
Expansion of the Clostridium perfringens toxin-based typing scheme., PMID:29866424
Mechanisms of Action and Cell Death Associated with Clostridium perfringens Toxins., PMID:29786671
Oral vaccination of broiler chickens against necrotic enteritis using a non-virulent NetB positive strain of Clostridium perfringens type A., PMID:29102330
Detection of a Newly Described Bacteriocin, Perfrin, Among Clostridium perfringens Isolates from Healthy and Diseased Ostriches and Broiler Chickens in Iran., PMID:28957009
Adhesion and invasion of Clostridium perfringens type A into epithelial cells., PMID:28720432
The Agr-Like Quorum Sensing System Is Required for Pathogenesis of Necrotic Enteritis Caused by Clostridium perfringens in Poultry., PMID:28373356
In ovo vaccination using Eimeria profilin and Clostridium perfringens NetB proteins in Montanide IMS adjuvant increases protective immunity against experimentally-induced necrotic enteritis., PMID:28335090
Multilocus sequence typing analyses of Clostridium perfringens type A strains harboring tpeL and netB genes., PMID:28238845
Experimental induction of necrotic enteritis in chickens by a netB-positive Japanese isolate of Clostridium perfringens., PMID:27980252
Design and expression of a chimeric vaccine candidate for avian necrotic enteritis., PMID:27887025