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Anti-NSP16 Polyclonal Antibody

Catalog #:   PVV02001 Specific References (12) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P0DTD1
Overview

Catalog No.

PVV02001

Species reactivity

SARS-CoV-2 (2019-nCoV)

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant SARS-CoV-2 NSP16 (Ser6799-Asn7096).

Tested applications

ELISA: 1:4000-1:8000, WB: 1:2000-1:8000

Target

2'-O-methyltransferase,Non-structural protein 16,nsp16,Replicase polyprotein 1ab,pp1ab,ORF1ab polyprotein

Purification

Purified by antigen affinity column.

Accession

P0DTD1

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with NSP16 antibody (PVV02001) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 61 kDa
    Observed MW: 61 kDa
References

A live attenuated SARS-CoV-2 vaccine constructed by dual inactivation of NSP16 and ORF3a., PMID:40132472

A repository of COVID-19 related molecular dynamics simulations and utilisation in the context of nsp10-nsp16 antivirals., PMID:37976980

Protein expression/secretion boost by a novel unique 21-mer cis-regulatory motif (Exin21) via mRNA stabilization., PMID:36793212

Targeting viral proteins for restraining SARS-CoV-2: focusing lens on viral proteins beyond spike for discovering new drug targets., PMID:36723288

Seasonal variation of the composition of essential oils from Piper cernuum Vell and Piper rivinoides Kunth, ADMET study, DFT calculations, molecular docking and dynamics studies of major components as potent inhibitors of the heterodimer methyltransferase complex NSP16-NSP10 SARS COV-2 protein., PMID:35943030

Intranasal administration of a single dose of a candidate live attenuated vaccine derived from an NSP16-deficient SARS-CoV-2 strain confers sterilizing immunity in animals., PMID:35352010

Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins., PMID:35216036

Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit., PMID:34452352

SARS-CoV-2 Proteins: Are They Useful as Targets for COVID-19 Drugs and Vaccines?, PMID:33622224

Inactivating Three Interferon Antagonists Attenuates Pathogenesis of an Enteric Coronavirus., PMID:32554697

Recent Advances in the Vaccine Development Against Middle East Respiratory Syndrome-Coronavirus., PMID:31428074

Identification and characterization of severe acute respiratory syndrome coronavirus replicase proteins., PMID:15331731

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-NSP16 Polyclonal Antibody [PVV02001]
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