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Anti-NSP13 Polyclonal Antibody

Catalog #:   PVV01801 Specific References (16) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P0DTD1
Overview

Catalog No.

PVV01801

Species reactivity

SARS-CoV-2 (2019-nCoV)

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant SARS-CoV-2 NSP13 (Ala5325-Gln5925).

Tested applications

ELISA: 1:4000-1:8000, WB: 1:2000-1:8000

Target

Helicase,Hel,Non-structural protein 13,nsp13,Replicase polyprotein 1ab,pp1ab,ORF1ab polyprotein

Purification

Purified by antigen affinity column.

Accession

P0DTD1

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with NSP13 antibody (PVV01801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 69 kDa
    Observed MW: 69 kDa
References

Mechanistic insights into bismuth(iii) inhibition of SARS-CoV-2 helicase., PMID:38966375

Heterotypic immunity from prior SARS-CoV-2 infection but not COVID-19 vaccination associates with lower endemic coronavirus incidence., PMID:38865483

Heterotypic responses against nsp12/nsp13 from prior SARS-CoV-2 infection associates with lower subsequent endemic coronavirus incidence., PMID:37961343

Porcine Epidemic Diarrhea Virus nsp13 Protein Downregulates Neonatal Fc Receptor Expression by Causing Promoter Hypermethylation through the NF-κB Signaling Pathway., PMID:36602596

Antibodies targeting conserved non-canonical antigens and endemic coronaviruses associate with favorable outcomes in severe COVID-19., PMID:35738278

Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected Cells., PMID:35730969

A Simplified SARS-CoV-2 Mouse Model Demonstrates Protection by an Oral Replicon-Based mRNA Vaccine., PMID:35250985

Bacteria-enabled oral delivery of a replicon-based mRNA vaccine candidate protects against ancestral and delta variant SARS-CoV-2., PMID:35123065

Highly feasible immunoprotective multicistronic SARS-CoV-2 vaccine candidate blending novel eukaryotic expression and Salmonella bactofection., PMID:35116175

RNAi-mediated siRNA sequences to combat the COVID-19 pandemic with the inhibition of SARS-CoV2., PMID:35071824

The impact of spike N501Y mutation on neutralizing activity and RBD binding of SARS-CoV-2 convalescent serum., PMID:34419925

Early induction of functional SARS-CoV-2-specific T cells associates with rapid viral clearance and mild disease in COVID-19 patients., PMID:33516277

Monoclonal Antibody: 5A9, 5C7, and 5G7 Against Porcine Epidemic Diarrhea Virus nsp13., PMID:33351713

Generation and Characterization of Monoclonal Antibody Against Porcine Epidemic Diarrhea Virus Nonstructural Protein 13., PMID:33351712

Analysis of intraviral protein-protein interactions of the SARS coronavirus ORFeome., PMID:17520018

Identification and characterization of severe acute respiratory syndrome coronavirus replicase proteins., PMID:15331731

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-NSP13 Polyclonal Antibody [PVV01801]
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