Catalog No.
KVV03501
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Rabies virus (RABV) Glycoprotein (G) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Rabies virus (RABV) Glycoprotein (G) present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for Rabies virus (RABV) Glycoprotein (G) is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Rabies virus (RABV) Glycoprotein (G) bound in the initial step. The color development is stopped and the intensity of the color is measured.
Specificity
Rabies virus (RABV) Glycoprotein (G)
Applications
Used for the quantitative determination of Rabies virus (RABV) Glycoprotein (G) concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
78.13 - 5,000 pg/mL
Sensitivity
32.11 pg/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
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|
Intra-Assay Precision
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Inter-Assay Precision
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|
Sample
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1
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2
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3
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1
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2
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3
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|
n
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16
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16
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16
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24
|
24
|
24
|
|
Mean (pg/mL)
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1993.5
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408.1
|
110.2
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2092.3
|
427.8
|
114.2
|
|
Standard deviation
|
75.2
|
25.1
|
7.3
|
74.9
|
20.3
|
8.2
|
|
CV (%)
|
3.8
|
6.2
|
6.7
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3.6
|
4.7
|
7.2
|
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃.
Engineered RVG-exosomes-mediated delivery of siRNAs targeting NMDAR alleviates orofacial neuropathic pain by suppressing central sensitivity., PMID:40482847
Development of an oral gut-targeted rabies virus-like particles (RVLPs) vaccine with mucosal immune adjuvant LTB via delivering of localized-release microparticles., PMID:40476513
Development of Salvianolic Acid A-Loaded Gelatin Nanoparticles for Effective Suppression of Inflammation and Oxidative Stress in Blood-Brain Barrier to Ischemic Stroke Therapy., PMID:40462595
Exposed nucleoprotein inside rabies virus particle as an ideal target for real-time quantitative evaluation of rabies virus particle integrity in vaccine quality control., PMID:40445993
Development of human targeted extracellular vesicles loaded with shRNA minicircles to prevent parkinsonian pathology., PMID:40420149
Deep mutational scanning of rabies glycoprotein defines mutational constraint and antibody-escape mutations., PMID:40398416
Development and validation of a blocking ELISA for measurement of rabies virus neutralizing antibody., PMID:40366189
Optimization of neuron-specific interfering peptides targeting GABAB receptor downregulation for proteolytic stability for conferring neuroprotection in a mouse model of cerebral ischemia., PMID:40356975
Infectious potential and circulation of SARS-CoV-2 in wild rats., PMID:40354427
Rabies vaccination induces a CD4+ TEM and CD4+CD8+ TEMRA TH1 phenotype in dogs., PMID:40354406
A transgenic mouse line for rabies virus-mediated trans-synaptic tracing in the postnatal developing brain., PMID:40354365
Intranasal Delivery of BACE1 siRNA and Berberine via Engineered Stem Cell Exosomes for the Treatment of Alzheimer's Disease., PMID:40351705
Protective antibody response in Korean raccoon dogs (Nyctereutes procynoide koreensis) administered a new rabies bait vaccine containing the ERAGS-GFP strain., PMID:40321792
Rabies Virus Targeting NIR-II Phototheranostics., PMID:40315345
Protection Against Rabies Induced by the Non-Replicative Viral Vectors MVA and Ad5 Expressing Rabies Glycoprotein., PMID:40284919
Microfluidics-Assisted Formulation of Polymeric Oxytocin Nanoparticles for Targeted Brain Delivery., PMID:40284447
Green-to-red spectral labeling: A novel polysynaptic retrograde tracing strategy in the marker footprint mouse model., PMID:40275744
Anatomical mapping of whole-brain monosynaptic inputs to the orbitofrontal cortex., PMID:40256320
Rabies virus large protein-derived T-cell immunogen facilitates rapid viral clearance and enhances protection against lethal challenge in mice., PMID:40251380
Selective inhibition of rabies virus gene expression by human miRNAs: a therapeutic approach using the 7mer-m8 model., PMID:40234303
Assessing anti-rabies vaccine response in humans: A rapid and high-throughput adaptable, pseudovirus-based neutralization assay as an alternative to rapid fluorescent focus inhibition test (RFFIT)., PMID:40208858
RVG engineered extracellular vesicles-transmitted miR-137 improves autism by modulating glucose metabolism and neuroinflammation., PMID:40175518
Engineered Extracellular Vesicles Loaded with MiR-100-5p Antagonist Selectively Target the Lesioned Region to Promote Recovery from Brain Damage., PMID:40167866
Molecular basis of two broad-spectrum antibodies neutralizing rabies virus and other phylogroup-I lyssaviruses by blocking structural transition between the pleckstrin-homology and fusion domains in the glycoprotein., PMID:40154685
Temporal and Spatial Analysis of Rabies Virus Lineages in South Africa., PMID:40143269
Construction and evaluation of recombinant rabies virus encoding three copies codon-optimized G genes as inactivated rabies vaccine in dogs and cats., PMID:40112690
Targeted Polymersomes Enable Enhanced Delivery to Peripheral Nerves Post-Injury., PMID:40068147
Intranasal delivery of AEP inhibitor-loaded neuron-targeted liposome ameliorates radiation-induced brain injury., PMID:40026629
Optimizing rabies mRNA vaccine efficacy via RABV-G structural domain screening and heterologous prime-boost immunization., PMID:40025078
A VSV-based oral rabies vaccine was sentineled by Peyer's patches and induced a timely and durable immune response., PMID:40022445
Changes in the dynamic characteristics of G-protein can alter the immune-protection efficacy of rabies virus vaccine., PMID:39982033
IL-16 exerts anti-rabies virus effects through CD9 on the surface of viral particles., PMID:39956243
Development of a blocking ELISA for evaluating neutralizing antibodies in human and canine serum based on rabies virus glycoprotein epitope I., PMID:39863206
A simple polydopamine-based platform for engineering extracellular vesicles with brain-targeting peptide and imaging probes to improve stroke outcome., PMID:39783851
Safety and immunogenicity of an optimized self-replicating RNA platform for low dose or single dose vaccine applications: a randomized, open label Phase I study in healthy volunteers., PMID:39774967
Evaluation of the Efficacy of the Vaccine Production Process in Removing Residual Host Cell DNA from the Vero Cell Rabies Vaccine., PMID:39772041
Deep mutational scanning of rabies glycoprotein defines mutational constraint and antibody-escape mutations., PMID:39763725
A nucleoside-modified rabies mRNA vaccine induces long-lasting and comprehensive immune responses in mice and non-human primates., PMID:39741409
In vivo self-assembled siRNAs within small extracellular vesicles attenuate LRRK2-induced neurodegeneration in Parkinson's disease models., PMID:39722304
ROS-responsive quercetin-based polydopamine nanoparticles for targeting ischemic stroke by attenuating oxidative stress and neuroinflammation., PMID:39675536
Circular RNA SCMH1 suppresses KMO expression to inhibit mitophagy and promote functional recovery following stroke., PMID:39659575
Widespread neuroanatomical integration and distinct electrophysiological properties of glioma-innervating neurons., PMID:39630872
Comparative Efficacy of Tumor Microenvironment-responsive Nanotherapeutics Targeting PSD95/Discs-large/ZO-1 Binding Kinase in Different Histological Subgroups of Medulloblastoma., PMID:39628686
Evaluation of the immune status of dogs vaccinated against rabies by an enzyme-linked immunosorbent assay using crude preparations of insect cells infected with a recombinant baculovirus encoding the rabies virus glycoprotein gene., PMID:39625902
Development of Human Monoclonal Antibodies With Broad Reactivity for Rabies Postexposure Prophylaxis., PMID:39601104
Rabies virus-mimicking liposomes for targeted gene therapy in Alzheimer's disease., PMID:39592065
Bioinformatics designing of an mRNA vaccine for Mokola virus (MOKV) using immunoinformatics as a secure strategy for successful vaccine development., PMID:39563241
Curcumin-Loaded Gelatin Nanoparticles Cross the Blood-Brain Barrier to Treat Ischemic Stroke by Attenuating Oxidative Stress and Neuroinflammation., PMID:39553455
IL-7 promotes mRNA vaccine-induced long-term immunity., PMID:39550592
Identification of clade-defining single nucleotide polymorphisms for improved rabies virus surveillance., PMID:39512853
For research use only. Not for human or drug use.
