Catalog No.
KAK24001
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative indirect enzyme immunoassay technique. Ds DNA has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-dsDNA Mouse IgG present is bound by the immobilized protein. After washing away any unbound substances, a HRP-labeled Goat Anti-Mouse IgG antibody is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-dsDNA Mouse IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Specificity
Anti-dsDNA Mouse IgG Antibody
Applications
Used for the quantitative determination of Anti-dsDNA Mouse IgG concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
1.56 - 100 ng/mL
Sensitivity
0.43 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
16
|
16
|
16
|
24
|
24
|
24
|
|
Mean (ng/mL)
|
51.6
|
13.4
|
2.7
|
44.5
|
11.7
|
2.6
|
|
Standard deviation
|
4.9
|
1.1
|
0.2
|
2.9
|
1.0
|
0.3
|
|
CV (%)
|
9.5
|
7.8
|
6.3
|
6.6
|
8.5
|
9.9
|
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Anti-mitochondrial antibodies as markers of disease activity in childhood-onset systemic lupus erythematosus: a longitudinal cohort study., PMID:40408235
A serum biomarker panel and miniarray detection system for tracking disease activity and flare risk in lupus nephritis., PMID:40375995
Characterization of anti-neutrophil extracellular traps (NET) antibodies according to systemic lupus erythematosus clinical phenotypes., PMID:40343598
Effects of telitacicept in SLE patients with antiphospholipid antibody positivity: a retrospective self-controlled case series., PMID:40317459
Neutrophil Z Is a Novel Marker to Differentiate Disease Flares From Bacterial Infections in Febrile SLE Patients., PMID:40302194
Collapsing Focal Segmental Glomerulosclerosis With Concurrent IgG4 Nephropathy., PMID:40264599
[The effects of S100A9 gene knockout on lupus-like phenotype in mice]., PMID:40260564
IgE autoantibodies to nuclear antigens in patients with different connective tissue diseases: re-evaluation and novel findings., PMID:40181984
Rheumatic antibodies may serve as indicators of disease severity, relapse frequency, and ocular involvement in NMOSD patients., PMID:40112464
Suppression of skin lesions and SLE nephritis by increasing Treg in MRL/FASlpr mice by administration of bee venom Apitoxin®., PMID:40108667
Ruxolitinib synergizes with regulatory T cells to improve inflammation but has no added benefits in decreasing albuminuria in SLE., PMID:39975551
Examining the lifespan of red blood cells in individuals with systemic lupus erythematosus., PMID:39973432
Autoantibody clusters in childhood-onset systemic lupus erythematosus: Insights from a multicenter study with 912 patients., PMID:39869699
CAR-T cell targeting three receptors on autoreactive B cells for systemic lupus erythematosus therapy., PMID:39832454
Targeting the Progression of Lupus-Like Disease in Humanized Mouse Model by Specific Dietary Components., PMID:39748653
Type I interferon-stimulated genes predict clinical response to belimumab in systemic lupus erythematosus., PMID:39672224
Fungal microbiome in gut of systemic lupus erythematosus (SLE)-prone mice (pristane and FCGRIIb deficiency), a possible impact of fungi in lupus., PMID:39637140
Short- and long-term pathologic responses to quartz are induced by nearly free silanols formed during crystal fracturing., PMID:39633374
Correlation of autoimmune response and immune system components in the progression of IgA nephropathy: A comparative study., PMID:39566436
Severe Antiphospholipid Syndrome and Diffuse Glomerulonephritis After Adalimumab Treatment in a Patient With Ulcerative Colitis., PMID:39529874
Comparative time-series analyses of gut microbiome profiles in genetically and chemically induced lupus-prone mice and the impacts of fecal transplantation., PMID:39487198
High prevalence of long COVID in anti-TPO positive euthyroid individuals with strongly elevated SARS-CoV-2-specific T cell responses and moderately raised anti-spike IgG levels 23 months post-infection., PMID:39450181
Threonine Phosphorylation and the Yin and Yang of STAT1: Phosphorylation-Dependent Spectrum of STAT1 Functionality in Inflammatory Contexts., PMID:39329714
The Diagnostic and Therapeutic Value of Anti CCP Antibodies and Double Stranded DNA in Rhupus Syndrome., PMID:39257108
Increased autoreactivity and maturity of EBI2+ antibody-secreting cells from nasal polyps., PMID:39253973
Progranulin mediates the onset of pristane induced systemic lupus erythematosus., PMID:39252120
Association of gut commensal translocation with autoantibody production in systemic lupus erythematosus., PMID:39250733
Demethylzeylasteral ameliorates podocyte damage in murine lupus by inhibiting inflammation and enhancing autophagy., PMID:39241387
Pseudo-Elevation of Anti Double Stranded DNA IgG Antibody Caused by Rheumatoid Factor., PMID:39193970
Phosphatidylserine-dependent antiprothrombin antibodies as a key predictor for systemic lupus erythematosus in patients with primary antiphospholipid syndrome: A retrospective longitudinal cohort study., PMID:39187460
CX-5461 ameliorates disease in lupus-prone mice by triggering B-cell ferroptosis via p53-SLC7A11-ALOX12 pathway., PMID:39111584
Dioscin attenuates lupus nephritis in NZB/W F1 mice by decreasing NF-κB activation and NLRP3 inflammasome., PMID:39042029
Mesenchymal stem cells-derived extracellular vesicles ameliorate lupus nephritis by regulating T and B cell responses., PMID:39020448
Deletion of the Mitochondrial Membrane Protein Fam210b Is Associated with the Development of Systemic Lupus Erythematosus., PMID:39000360
Dihydroartemisinin inhibits follicular helper T and B cells: implications for systemic lupus erythematosus treatment., PMID:38977652
Is systemic lupus erythematosus linked to Immunoglobulin G4 Autoantibodies?, PMID:38954949
[Humoral Immunity Abnormalities in Advanced Maternal-Age Women With Recurrent Spontaneous Abortion: A Single Center Study]., PMID:38948271
Anti-dsDNA IgE: a potential non-invasive test for prediction of lupus nephritis relapse., PMID:38942591
Inhibition of receptor interacting protein kinase-1 (RIPK1) in the treatment of murine lupus., PMID:38906550
Effects of belimumab on the lipid profile in systemic lupus erythematosus patients: an observational study., PMID:38877375
Successful Desensitization with Imlifidase and Daratumumab in a Highly Immunized, Crossmatch Positive, Blood Group-Incompatible Living-Donor Re-Transplant Recipient with Systemic Lupus Erythematosus and Antiphospholipid Syndrome., PMID:38867806
IgG4-associated disease with systemic lupus erythematosus: A case report and review., PMID:38836366
Blockade of OX40/OX40L signaling using anti-OX40L alleviates murine lupus nephritis., PMID:38798163
Efficacy and safety of telitacicept therapy in systemic lupus erythematosus with hematological involvement., PMID:38767710
Clinical Relevance of Anti-C3 and Anti-C4 Autoantibodies in Lupus Nephritis., PMID:38707805
Rare complication of rheumatoid arthritis: Charcot Neuro-osteoarthropathy., PMID:38685038
Prothrombotic autoantibodies targeting platelet factor 4/polyanion are associated with pediatric cerebral malaria., PMID:38652559
Zhen-Wu-Tang ameliorates lupus nephritis by diminishing renal tissue-resident memory CD8+ T cells via suppressing IL-15/STAT3 pathway., PMID:38643544
Efficacy and safety of telitacicept in patients with lupus nephritis: a single-center, real-world retrospective study., PMID:38613741
MiR-574-5p activates human TLR8 to promote autoimmune signaling and lupus., PMID:38589923
For research use only. Not for human or drug use.
