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Anti-SARS-CoV-2 Spike Protein (BA.5) Human IgA ELISA Kit

Catalog #:   KAV00107 Specific References (0) DATASHEET
Applications: Used for the quantitative determination of Anti-SARS-CoV-2 Spike Protein (BA.5) Human IgA concentration in serum and plasma.
Sample type: Plasma, Serum
Sensitivity: 0.41 ng/mL
Range: 1.56 - 100 ng/mL
Overview

Catalog No.

KAV00107

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant SARS-CoV-2 Spike Protein (BA.5) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-SARS-CoV-2 Spike Protein (BA.5) Human IgA present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgA antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-SARS-CoV-2 Spike Protein (BA.5) Human IgA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Specificity

Anti-SARS-CoV-2 Spike Protein (BA.5) Human IgA Antibody

Applications

Used for the quantitative determination of Anti-SARS-CoV-2 Spike Protein (BA.5) Human IgA concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

1.56 - 100 ng/mL

Sensitivity

0.41 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

 

Intra-Assay Precision

Inter-Assay Precision

Sample

1

2

3

1

2

3

n

16

16

16

24

24

24

Mean (ng/mL)

45.8

12.9

3.0

53.0

12.9

3.0

Standard deviation

3.7

0.5

0.2

2.5

0.4

0.2

CV (%)

8.1

3.7

6.1

4.8

3.2

5.5

 

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

Data Image
References
Datasheet
$ 1128
Product specifications
96 T 1128

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-SARS-CoV-2 Spike Protein (BA.5) Human IgA ELISA Kit [KAV00107]
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