Catalog No.
YVV39801
Expression system
E. coli
Species
Japanese encephalitis virus (strain SA-14) (JEV)
Protein length
Pro1685-Arg2123
Predicted molecular weight
51.69 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P27395
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Genome polyprotein, Serine protease NS3, 3.4.21.91, 3.6.1.15, 3.6.4.13, Flavivirin protease NS3 catalytic subunit, Non-structural protein 3
The interaction of Orthoflavivirus nonstructural proteins 3 and 5 with human fatty acid synthase., PMID:40131913
Identification of novel thiazole derivatives as flaviviral protease inhibitors effective against Dengue (DENV2) and Japanese encephalitis viruses., PMID:39992103
Japanese encephalitis virus NS3 captures the protein translation element by interacting with HNRNPH1 to promote viral replication., PMID:39694377
NS2B-D55E and NS2B-E65D Variations Are Responsible for Differences in NS2B-NS3 Protease Activities Between Japanese Encephalitis Virus Genotype I and III in Fluorogenic Peptide Model., PMID:39684391
Cellular NONO protein binds to the flavivirus replication complex and promotes positive-strand RNA synthesis., PMID:39499073
NS2B-D55E and NS2B-E65D Variations are Responsible for Differences in NS2B-NS3 Protease Activities Between Japanese Encephalitis Virus Genotype I and III in Fluorogenic Peptide Model., PMID:38105993
Integrative RNA profiling of TBEV-infected neurons and astrocytes reveals potential pathogenic effectors., PMID:35685361
C19orf66 Inhibits Japanese Encephalitis Virus Replication by Targeting -1 PRF and the NS3 Protein., PMID:34309824
Mechanism through Which Retrocyclin Targets Flavivirus Multiplication., PMID:33980602
Monoclonal Antibody That Inhibits Cleavage Activity of Japanese Encephalitis Virus NS3., PMID:33625288
Identification of Cleavage Sites Proteolytically Processed by NS2B-NS3 Protease in Polyprotein of Japanese Encephalitis Virus., PMID:33494395
Degradation of MicroRNA miR-466d-3p by Japanese Encephalitis Virus NS3 Facilitates Viral Replication and Interleukin-1β Expression., PMID:32461319
A novel anti-NS2BNS3pro antibody-based indirect ELISA test for the diagnosis of dengue virus infections., PMID:32418268
Natural chemical entities from Arisaema genus might be a promising break-through against Japanese encephalitis virus infection: a molecular docking and dynamics approach., PMID:32072856
Hsp40 Protein DNAJB6 Interacts with Viral NS3 and Inhibits the Replication of the Japanese Encephalitis Virus., PMID:31739611
Japanese encephalitis virus: A multi-epitope loaded peptide vaccine formulation using reverse vaccinology approach., PMID:31706079
In Vivo Dynamics of Reporter Flaviviridae Viruses., PMID:31462560
NS2B/NS3 mutations enhance the infectivity of genotype I Japanese encephalitis virus in amplifying hosts., PMID:31381617
Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro., PMID:31212927
Molecular interaction of the antiviral compound CW‑33 and its analogues with the NS2B‑NS3 protease of the Japanese encephalitis virus., PMID:30816489
Pharmacophore anchor models of flaviviral NS3 proteases lead to drug repurposing for DENV infection., PMID:29297305
Erythrosin B is a potent and broad-spectrum orthosteric inhibitor of the flavivirus NS2B-NS3 protease., PMID:29288700
The NS3 and NS4A genes as the targets of RNA interference inhibit replication of Japanese encephalitis virus in vitro and in vivo., PMID:27593564
Transmembrane Domains of NS2B Contribute to both Viral RNA Replication and Particle Formation in Japanese Encephalitis Virus., PMID:27053551
Guiding dengue vaccine development using knowledge gained from the success of the yellow fever vaccine., PMID:26435066
[Method for Japanese encephalitis virus NS3 protease activity analysis and high-throughput screening assay for inhibitors]., PMID:24941741
Japanese encephalitis virus vaccine candidates generated by chimerization with dengue virus type 4., PMID:24699473
Methods for detecting ATP hydrolysis and nucleic acid unwinding of Japanese encephalitis virus NS3 helicase., PMID:23948158
Antiviral potential of 4-hydroxypanduratin A, secondary metabolite of Fingerroot, Boesenbergia pandurata (Schult.), towards Japanese Encephalitis virus NS2B/NS3 protease., PMID:23390345
The NS3 protease and helicase domains of Japanese encephalitis virus trigger cell death via caspase‑dependent and ‑independent pathways., PMID:23291778
Enzymatic analysis of recombinant Japanese encephalitis virus NS2B(H)-NS3pro protease with fluorogenic model peptide substrates., PMID:22615830
Development of a fluorescent-intercalating-dye-based reverse transcription loop-mediated isothermal amplification assay for rapid detection of seasonal Japanese B encephalitis outbreaks in pigs., PMID:22573187
Monoclonal antibodies against NS3 and NS5 proteins of Japanese encephalitis virus., PMID:22509919
Characterization of nonstructural protein 3 of a neurovirulent Japanese encephalitis virus strain isolated from a pig., PMID:21549011
Structure-based virtual screening for novel inhibitors of Japanese encephalitis virus NS3 helicase/nucleoside triphosphatase., PMID:19863664
Japanese encephalitis virus NS2B-NS3 protease induces caspase 3 activation and mitochondria-mediated apoptosis in human medulloblastoma cells., PMID:19463724
Viral NS3 helicase activity is inhibited by peptides reproducing the Arg-rich conserved motif of the enzyme (motif VI)., PMID:18479669
Crystal structure of the catalytic domain of Japanese encephalitis virus NS3 helicase/nucleoside triphosphatase at a resolution of 1.8 A., PMID:18201743
Functional determinants of NS2B for activation of Japanese encephalitis virus NS3 protease., PMID:17467838
Organization of flaviviral replicase proteins in virus-induced membranes: a role for NS1' in Japanese encephalitis virus RNA synthesis., PMID:17319159
Inhibitory effect of RNAi on Japanese encephalitis virus replication in vitro and in vivo., PMID:16365529
Japanese encephalitis virus NS2B-NS3 protease binding to phage-displayed human brain proteins with the domain of trypsin inhibitor and basic region leucine zipper., PMID:16289409
Conserved amino acids 193-324 of non-structural protein 3 are a dominant source of peptide determinants for CD4+ and CD8+ T cells in a healthy Japanese encephalitis virus-endemic cohort., PMID:15105530
Impaired T helper 1 function of nonstructural protein 3-specific T cells in Japanese patients with encephalitis with neurological sequelae., PMID:14976606
Cell-mediated immune responses in healthy children with a history of subclinical infection with Japanese encephalitis virus: analysis of CD4+ and CD8+ T cell target specificities by intracellular delivery of viral proteins using the human immunodeficiency virus Tat protein transduction domain., PMID:14769905
Association of Japanese encephalitis virus NS3 protein with microtubules and tumour susceptibility gene 101 (TSG101) protein., PMID:13679614
Screening for T cell-eliciting proteins of Japanese encephalitis virus in a healthy JE-endemic human cohort using recombinant baculovirus-infected insect cell preparations., PMID:12898332
Characterization of RNA synthesis, replication mechanism, and in vitro RNA-dependent RNA polymerase activity of Japanese encephalitis virus., PMID:12667804
Role of the DExH motif of the Japanese encephalitis virus and hepatitis C virus NS3 proteins in the ATPase and RNA helicase activities., PMID:10915602
Identification and characterization of the RNA helicase activity of Japanese encephalitis virus NS3 protein., PMID:10620709
For research use only. Not for human or drug use.
