Catalog No.
YXX08404
Expression system
E. coli
Species
Streptococcus pyogenes
Protein length
Met1-Asp1368
Predicted molecular weight
159.92 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q99ZW2
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
CRISPR-associated endonuclease Cas9/Csn1, 3.1.-.-, SpCas9, SpyCas9, cas9, csn1, SPy_1046
Crystal structure and inhibition mechanism of AcrIIA11., PMID:40414010
Functional insights from recombinant production of bacterial proteases in Saccharomyces cerevisiae., PMID:40405239
Structural and dynamic impacts of single-atom disruptions to guide RNA interactions within the recognition lobe of Geobacillus stearothermophilus Cas9., PMID:40387084
Evaluation of Immunostimulatory Effects of Bacterial Lysate Proteins on THP-1 Macrophages: Pro-inflammatory Cytokine Response and Proteomic Profiling., PMID:40322557
Structure-Based Classification of CRISPR/Cas9 Proteins: A Machine Learning Approach to Elucidating Cas9 Allostery., PMID:40291704
Enhanced production of functional CRISPR-AsCas12a protein in Escherichia coli., PMID:40288547
Mechanism of Cas9 inhibition by AcrIIA11., PMID:40277083
Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing., PMID:40273916
Spy-ing on nucleic acids: Atomic resolution of the S. pyogenes CRISPR-Cas9 surveillance state., PMID:40185080
Heritable virus-induced germline editing in tomato., PMID:40163287
Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner., PMID:40156040
Energetics of Expanded PAM Readability by Engineered Cas9-NG., PMID:40146191
Protein Carrier Adeno-Associated Virus., PMID:40117458
Implementation of RAGATH RNA-associated DNA Endonucleases as Genome Editing Tool in Escherichia coli., PMID:40111000
Genome editing in cells of apple cultivar 'Fuji' using geminivirus-derived replicons for transient expression of CRISPR/Cas9 components., PMID:40083569
Insight into crRNA Processing in Streptococcus mutans P42S and Application of SmutCas9 in Genome Editing., PMID:40076628
Characterization of diverse Cas9 orthologs for genome and epigenome editing., PMID:40073054
Improving adenine base editing precision by enlarging the recognition domain of CRISPR-Cas9., PMID:40021632
Minimally Humanized Ezh2 Exon-18 Mouse Cell Lines Validate Preclinical CRISPR/Cas9 Approach to Treat Weaver Syndrome., PMID:39964768
Construction of a CRISPR-Cas9-Based Genetic Editing Tool for Serratia marcescens Using a Stationary Phase Promoter and Its Application in Putrescine Production., PMID:39910976
Omics-based identification of the broader effects of 2-hydroxyisoflavanone synthase gene editing on a gene regulatory network beyond isoflavonoid loss in soybean hairy roots., PMID:39786412
Enhanced Genome Editing Activity with Novel Chimeric ScCas9 Variants in Rice., PMID:39755933
Efficient genome editing in medaka (Oryzias latipes) using a codon-optimized SaCas9 system., PMID:39743295
Conformational transitions of Streptococcus pyogenes Cas9 induced by salt and single-guide RNA binding., PMID:39716488
Orthogonal transcriptional modulation and gene editing using multiple CRISPR-Cas systems., PMID:39563029
Engineering CjCas9 for Efficient Base Editing and Prime Editing., PMID:39556313
Quantifying Protein-Nucleic Acid Interactions for Engineering Useful CRISPR-Cas9 Genome-Editing Variants., PMID:39543038
Precise Base Substitution Using CRISPR/Cas-Mediated Base Editor in Rice., PMID:39499471
A Nanobody Toolbox for Recognizing Distinct Epitopes on Cas9., PMID:39481635
Protein Carrier AAV., PMID:39185209
Expanding the Genome-Editing Toolbox with Abyssicoccus albus Cas9 Using a Unique Protospacer Adjacent Motif Sequence., PMID:39111827
Developing small Cas9 hybrids using molecular modeling., PMID:39060399
A dynamic subpopulation of CRISPR-Cas overexpressers allows Streptococcus pyogenes to rapidly respond to phage., PMID:38997519
[The Development of SpCas9 Variants with High Specificity and Efficiency Based on the HH Theory]., PMID:38943587
PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics., PMID:38942756
Recent Updates of the CRISPR/Cas9 Genome Editing System: Novel Approaches to Regulate Its Spatiotemporal Control by Genetic and Physicochemical Strategies., PMID:38859956
Identification of Family-Specific Features in Cas9 and Cas12 Proteins: A Machine Learning Approach Using Complete Protein Feature Spectrum., PMID:38838358
Structural basis for pegRNA-guided reverse transcription by a prime editor., PMID:38811740
Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9., PMID:38688943
Application of Cas12j for Streptomyces Editing., PMID:38672502
How to use CRISPR/Cas9 in plants: from target site selection to DNA repair., PMID:38648173
Why Does the E1219V Mutation Expand T-Rich PAM Recognition in Cas9 from Streptococcus pyogenes?, PMID:38600752
Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania., PMID:38558208
Expanding the flexibility of base editing for high-throughput genetic screens in bacteria., PMID:38499498
RNA Switches Using Cas Proteins., PMID:38441765
Functional analysis of single enzymes combining programmable molecular circuits with droplet-based microfluidics., PMID:38409552
Engineering Cas9: next generation of genomic editors., PMID:38353732
An Efficient Expression and Purification Protocol for SpCas9 Nuclease and Evaluation of Different Delivery Methods of Ribonucleoprotein., PMID:38338898
Use of Cas9 Targeting and Red Recombination for Designer Phage Engineering., PMID:38300409
Targeted mutagenesis in mice via an engineered AsCas12f1 system., PMID:38280977
For research use only. Not for human or drug use.
