Overview
Catalog No.
PHJ90401
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human PSAT1 (Met1-Gly312).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Phosphohydroxythreonine aminotransferase,PSAT,PSAT1,PSA,Phosphoserine aminotransferase
Purification
Purified by antigen affinity column.
Accession
Q9Y617
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Data Image
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Various lysates were subjected to SDS PAGE followed by western blot with PSAT1 antibody (PHJ90401) at 0.62μg/ml.
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 40 kDa
Observed MW: 40 kDa
-
Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PSAT1 antibody (PHJ90401) at 1 µg/ml.
Lane 1: Recombinant Protein
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 36 kDa
Observed MW: 36 kDa
References
PSAT1 impairs ferroptosis and reduces immunotherapy efficacy via GPX4 hydroxylation., PMID:40281343
In search of autophagy biomarkers in breast cancer: Receptor status and drug agnostic transcriptional changes during autophagy flux in cell lines., PMID:34990474
Interleukin-6 mediates PSAT1 expression and serine metabolism in TSC2-deficient cells., PMID:34544857
Overexpression of PSAT1 regulated by G9A sustains cell proliferation in colorectal cancer., PMID:32300099
Identification of differential proteins in colorectal cancer cells treated with caffeic acid phenethyl ester., PMID:25206290
Expression of serine and glycine-related enzymes in phyllodes tumor., PMID:25030440
Datasheet
For research use only. Not for human or drug use.
