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Anti-POMP Polyclonal Antibody

Catalog #:   PHJ84601 Specific References (26) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9Y244
Overview

Catalog No.

PHJ84601

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human POMP (Met1-Leu141).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

UMP1,hUMP1,Voltage-gated K channel beta subunit 4.1,Protein UMP1 homolog,Proteassemblin,Proteasome maturation protein,C13orf12,POMP

Purification

Purified by antigen affinity column.

Accession

Q9Y244

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with POMP antibody (PHJ84601) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 18 kDa
    Observed MW: 18 kDa
  • Immunocytochemistry/ Immunofluorescence
    POMP in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with POMP (PHJ84601) at 10 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-594) preabsorbed at 1/100 dilution (Shown in red) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Mini-hyper-CVD plus inotuzumab ozogamicin, with or without blinatumomab, in the subgroup of older patients with newly diagnosed Philadelphia chromosome-negative B-cell acute lymphocytic leukaemia: long-term results of an open-label phase 2 trial., PMID:37187201

Latency of Symptom Progression in Mild Daboia palaestinae Envenomation., PMID:35466015

SWOG 1318: A Phase II Trial of Blinatumomab Followed by POMP Maintenance in Older Patients With Newly Diagnosed Philadelphia Chromosome-Negative B-Cell Acute Lymphoblastic Leukemia., PMID:35157496

Hyper-CVAD regimen in combination with ofatumumab as frontline therapy for adults with Philadelphia chromosome-negative B-cell acute lymphoblastic leukaemia: a single-arm, phase 2 trial., PMID:32589978

Directed Differentiation of Human Corneal Endothelial Cells From Human Embryonic Stem Cells by Using Cell-Conditioned Culture Media., PMID:30025120

Inotuzumab ozogamicin in combination with low-intensity chemotherapy for older patients with Philadelphia chromosome-negative acute lymphoblastic leukaemia: a single-arm, phase 2 study., PMID:29352703

Single agent blinatumumab as frontline therapy for an 85-year-old patient with B cell precursor acute lymphoblastic leukemia., PMID:27468851

Host genetics and diet, but not immunoglobulin A expression, converge to shape compositional features of the gut microbiome in an advanced intercross population of mice., PMID:25516416

Phagocytosis: history's lessons., PMID:23427369

Identification of immunologically relevant proteins of Chlamydophila abortus using sera from experimentally infected pregnant ewes., PMID:20554807

[Use of argatroban during cardiopulmonary bypass for atrial septal defect with heparin induced thrombocytopenia after introduction of dialysis]., PMID:19202931

High prevalence of antibodies against Chlamydiaceae and Chlamydophila abortus in wild ungulates using two "in house" blocking-ELISA tests., PMID:19010612

Prognostic significance of CD20 expression in adults with de novo precursor B-lineage acute lymphoblastic leukemia., PMID:18703706

The proteasome maturation protein POMP facilitates major steps of 20S proteasome formation at the endoplasmic reticulum., PMID:17948026

Possible tetramerisation of the proteasome maturation factor POMP/proteassemblin/hUmp1 and its subcellular localisation., PMID:16624403

Polymorphic outer-membrane proteins of Chlamydophila abortus are glycosylated., PMID:11739762

The hyper-CVAD regimen in adult acute lymphocytic leukemia., PMID:11147229

P53 and radiotherapy for oesophageal carcinoma: A comparison between 4 different antibodies., PMID:10948341

Immunoelectron microscopic localisation of the OMP90 family on the outer membrane surface of Chlamydia psittaci., PMID:9675857

Molecular cloning and characterization of the genes coding for the highly immunogenic cluster of 90-kilodalton envelope proteins from the Chlamydia psittaci subtype that causes abortion in sheep., PMID:9529048

Potato root diffusate-induced secretion of soluble, basic proteins originating from the subventral esophageal glands of potato cyst nematodes., PMID:18945052

Secretory granule proteins from the subventral esophageal glands of the potato cyst nematode identified by monoclonal antibodies to a protein fraction from second-stage juveniles., PMID:8589421

Increased translocation of Escherichia coli and development of arthritis in vitamin A-deficient rats., PMID:7622231

A new phenotypic classification of bone marrow plasmacytosis., PMID:8547606

In vitro and in vivo activities of monoclonal antibody-alkaline phosphatase conjugates in combination with phenol mustard phosphate., PMID:1790175

Monoclonal antibodies against rat glutathione S-transferase isoenzymes 2-2 and 3-3., PMID:2777277

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-POMP Polyclonal Antibody [PHJ84601]
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