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Anti-Baker's yeast SSB1 Polyclonal Antibody

Catalog #:   PXX54101 Specific References (9) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P11484
Overview

Catalog No.

PXX54101

Species reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Baker's yeast SSB1 (Met1-Arg613).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:1000-1:4000

Target

Ribosome-associated molecular chaperone SSB1, 3.6.4.10, Cold-inducible protein YG101, Heat shock protein SSB1, Hsp70 chaperone Ssb, SSB1, YG101, YDL229W

Concentration

0.84 mg/ml

Purification

Purified by antigen affinity column.

Accession

P11484

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with SSB1 antibody (PXX54101) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 69 kDa
    Observed MW: 69 kDa
References

The organization of ribosomal RNA processing correlates with the distribution of nucleolar snRNAs., PMID:8799814

Inactivation of the yeast Sen1 protein affects the localization of nucleolar proteins., PMID:8544822

Detection and expression of the 70 kDa heat shock protein SSB1P at different temperatures in Saccharomyces cerevisiae., PMID:7646503

Structural alterations of the nucleolus in mutants of Saccharomyces cerevisiae defective in RNA polymerase I., PMID:8455621

Purification of ribonucleoproteins by a novel approach: isolation of the SSB1 ribonucleoprotein from yeast and demonstration that it has no role in mRNA splicing., PMID:1482680

NOP3 is an essential yeast protein which is required for pre-rRNA processing., PMID:1429834

GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast., PMID:1531632

SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs., PMID:2121740

Multiple species of single-stranded nucleic acid-binding proteins in Saccharomyces cerevisiae., PMID:3905814

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Baker's yeast SSB1 Polyclonal Antibody [PXX54101]
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