Catalog No.
YHN50001
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Leu284-Asp379
Predicted molecular weight
39.02 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9H488
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
GDP-fucose protein O-fucosyltransferase 1, 2.4.1.221, Peptide-O-fucosyltransferase 1, O-FucT-1, POFUT1, FUT12, KIAA0180
Protein O-Fucosyltransferases: Biological Functions and Molecular Mechanisms in Mammals., PMID:40286076
Protein-O-fucosylation of coreceptors may be required for Nodal signaling in Xenopus., PMID:40043779
Potential drug targets for ovarian cancer identified through Mendelian randomization and colocalization analysis., PMID:39972314
FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains., PMID:39775168
Analysis of endogenous NOTCH1 from POFUT1 S162L patient fibroblasts reveals the importance of the O-fucose modification on EGF12 in human development., PMID:38976017
Protein O-fucosyltransferase 1 promotes PD-L1 stability to drive immune evasion and directs liver cancer to immunotherapy., PMID:38908854
Analysis of endogenous NOTCH1 from POFUT1 S162L patient fibroblasts reveals the importance of the O -fucose modification on EGF12 in human development., PMID:38645096
POFUT1 and PLAGL2 are characteristic markers of mucinous colorectal cancer associated with MUC2 expression., PMID:38500386
Endothelial POFUT1 controls injury-induced liver fibrosis by repressing fibrinogen synthesis., PMID:38460791
CircPOFUT1 fosters colorectal cancer metastasis and chemoresistance via decoying miR-653-5p/E2F7/WDR66 axis and stabilizing BMI1., PMID:38230259
POFUT1-mediated O-fucosylation of glycoproteins expressed in the baculovirus Sf9 insect cell expression system., PMID:38070779
EOGT enables residual Notch signaling in mouse intestinal cells lacking POFUT1., PMID:37838775
Synergistic regulation of Notch signaling by different O-glycans promotes hematopoiesis., PMID:37795096
A loss-of-function NCSTN mutation associated with familial Dowling Degos disease and hidradenitis suppurativa., PMID:37665193
Overexpression of POFUT1 promotes malignant phenotype and mediates perineural invasion in head and neck squamous cell carcinoma., PMID:37641160
POFUT1 promotes gastric cancer progression through Notch/Wnt dual signaling pathways dependent on the parafibromin-NICD1-β-catenin complex., PMID:37501238
Preliminary study on the mechanism of POFUT1 in colorectal cancer., PMID:37432515
O-Fucosylation of BMP1 promotes endometrial decidualization by activating BMP/Smad signaling pathway., PMID:37338142
In silico modelling of the function of disease-related CAZymes., PMID:36912236
Fucosyltransferases Regulated by Fusobacterium Nucleatum and Act as Novel Biomarkers in Colon Adenocarcinoma., PMID:36852302
KRT5 mutation regulate melanin metabolism through notch signalling pathway between keratinocytes and melanocytes., PMID:36809573
Role of poFUT1 and O-fucosylation in placental angiogenesis†., PMID:36723873
1H, 15N, 13C backbone and sidechain resonance assignments and secondary structure of mouse NOTCH1 EGF27., PMID:36565355
Cancer-associated Notch receptor variants lead to O-fucosylation defects that deregulate Notch signaling., PMID:36265581
Generation of Properly Folded Epidermal Growth Factor-Like (EGF) Repeats and Glycosyltransferases Enables In Vitro O-Glycosylation., PMID:35674889
Fringe GlcNAc-transferases differentially extend O-fucose on endogenous NOTCH1 in mouse activated T cells., PMID:35623385
Identification, function, and biological relevance of POGLUT2 and POGLUT3., PMID:35411374
A case report of Dowling-Degos disease caused by POFUT1 exon deletion with possible coexistent CARD14 mutation-related psoriasis., PMID:35340079
Comprehensive bioinformation analysis of differentially expressed genes in recurrent pregnancy loss., PMID:35306956
Asparagine Tautomerization in Glycosyltransferase Catalysis. The Molecular Mechanism of Protein O-Fucosyltransferase 1., PMID:34868727
Cell Models for Chromosome 20q11.21 Amplification and Drug Sensitivities in Colorectal Cancer., PMID:34577783
Congenital disorders of glycosylation with defective fucosylation., PMID:34389986
POFUT1 acts as a tumor promoter in glioblastoma by enhancing the activation of Notch signaling., PMID:34251584
Protein O-Fucosyltransferase 1 Undergoes Interdomain Flexibility in Solution., PMID:33916911
Hyperdense Pancreatic Ductal Adenocarcinoma: Clinical Characteristics and Proteomic Landscape., PMID:33718237
In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions., PMID:33636132
Dowling-Degos disease: a review., PMID:33368260
Diseases related to Notch glycosylation., PMID:33341260
Mouse WIF1 Is Only Modified with O-Fucose in Its EGF-like Domain III Despite Two Evolutionarily Conserved Consensus Sites., PMID:32872229
Association Analysis between SPP1, POFUT1 and PRLR Gene Variation and Milk Yield, Composition and Coagulation Traits in Sarda Sheep., PMID:32708940
The single EGF-like domain of mouse PAMR1 is modified by O-Glucose, O-Fucose and O-GlcNAc., PMID:32518939
POFUT1 is dispensable for structure, function and survival of mouse podocytes., PMID:32509213
Expression, purification, and glycosylation of epidermal growth factor-like repeat 27 from mouse NOTCH1., PMID:32505675
Functional Characterization of POFUT1 Variants Associated with Colorectal Cancer., PMID:32486426
Upregulation of Fucosyltransferase 3, 8 and protein O-Fucosyltransferase 1, 2 genes in esophageal cancer stem-like cells (CSLCs)., PMID:32157457
Epiregulin promotes trophoblast epithelial-mesenchymal transition through poFUT1 and O-fucosylation by poFUT1 on uPA., PMID:31889361
Glycosylation of Specific Notch EGF Repeats by O-Fut1 and Fringe Regulates Notch Signaling in Drosophila., PMID:31722217
Weighted gene coexpression analysis indicates that PLAGL2 and POFUT1 are related to the differential features of proximal and distal colorectal cancer., PMID:31638246
poFUT1 promotes uterine angiogenesis and vascular remodeling via enhancing the O-fucosylation on uPA., PMID:31601791
A modifier in the 129S2/SvPasCrl genome is responsible for the viability of Notch1[12f/12f] mice., PMID:31590629
For research use only. Not for human or drug use.
