Anti-SPHK1 Polyclonal Antibody

Catalog No.

PHJ68401

Species reactivity

Human, Mouse

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SPHK1 (Ser148-Leu384).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

SPHK,SK 1,SPK,Acetyltransferase SPHK1,Sphingosine kinase 1,SPHK1,SPK 1,SK1

Purification

Purified by antigen affinity column.

Accession

Q9NYA1

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

EGFR-mediated local invasiveness and response to Cetuximab in head and neck cancer., PMID:40121428

SPHK1 promotes HNSCC immune evasion by regulating the MMP1-PD-L1 axis., PMID:39629135

Sphingosine-1-phosphate alleviates Sjögren's syndrome-like symptoms via inducing autophagy and regulating status of Treg cells in NOD mice., PMID:39510034

Assessment of the Effects of Sphingosine Kinase 1/Sphingosine-1-Phosphate on Microangiogenesis at Rat Myofascial Trigger Points Using Contrast-Enhanced Ultrasonography., PMID:38371480

The Oncogenic Lipid Sphingosine-1-Phosphate Impedes the Phagocytosis of Tumor Cells by M1 Macrophages in Diffuse Large B Cell Lymphoma., PMID:38339325

Sphingosine kinase 1 promotes tumor immune evasion by regulating the MTA3-PD-L1 axis., PMID:36050478

RNA Sequencing Reveals Beneficial Effects of Atorvastatin on Endothelial Cells in Acute Kawasaki Disease., PMID:35861833

HCV inhibits M2a, M2b and M2c macrophage polarization via HCV core protein engagement with Toll-like receptor 2., PMID:35837038

Tumor Derived Extracellular Vesicles Drive T Cell Exhaustion in Tumor Microenvironment through Sphingosine Mediated Signaling and Impacting Immunotherapy Outcomes in Ovarian Cancer., PMID:35289120

Inhibition of sphingosine 1-phosphate protects mice against chondrocyte catabolism and osteoarthritis., PMID:34144150

Sphingosine kinase 1 regulates lysyl oxidase through STAT3 in hyperoxia-mediated neonatal lung injury., PMID:33883249

Proteome profiling reveals the efficacy and targets of sophocarpine against asthma., PMID:33857804

Leucine-rich Repeats and Immunoglobulin 1 (LRIG1) Ameliorates Liver Fibrosis and Hepatic Stellate Cell Activation via Inhibiting Sphingosine Kinase 1 (SphK1)/Sphingosine-1-Phosphate (S1P) Pathway., PMID:33463106

Sphingosine Kinase 1/S1P Signaling Contributes to Pulmonary Fibrosis by Activating Hippo/YAP Pathway and Mitochondrial Reactive Oxygen Species in Lung Fibroblasts., PMID:32192225

Resistance of melanoma to immune checkpoint inhibitors is overcome by targeting the sphingosine kinase-1., PMID:31974367

Sphingosine kinase-1 predicts overall survival outcomes in non-small cell lung cancer patients treated with carboplatin and navelbine., PMID:31423186

MicroRNA-28-5p regulates glioma cell proliferation, invasion and migration by targeting SphK1., PMID:31378904

Sphingosine-1-phosphate signalling drives an angiogenic transcriptional programme in diffuse large B cell lymphoma., PMID:31097785

Thrombin Contributes to Anti-myeloperoxidase Antibody Positive IgG-Mediated Glomerular Endothelial Cells Activation Through SphK1-S1P-S1PR3 Signaling., PMID:30891029

Sphingosine kinase 1 overexpression contributes to sunitinib resistance in clear cell renal cell carcinoma., PMID:30524898

Activation of the SphK1/ERK/p-ERK pathway promotes autophagy in colon cancer cells., PMID:29928348

Sphingosine-1-phosphate mediates the therapeutic effects of bone marrow mesenchymal stem cell-derived microvesicles on articular cartilage defect., PMID:29324234

Resveratrol and inflammatory bowel disease: the evidence so far., PMID:29191255

Crosstalk between TLR2 and Sphk1 in microglia in the cerebral ischemia/reperfusion-induced inflammatory response., PMID:29039449

Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines., PMID:28560513

Interaction between transcription factors PAX6/PAX6-5a and specific members of miR-183-96-182 cluster, may contribute to glioma progression in glioblastoma cell lines., PMID:28184912

Sphingosine Kinase 2 Deficiency Attenuates Kidney Fibrosis via IFN-γ., PMID:27799486

Essential role for SphK1/S1P signaling to regulate hypoxia-inducible factor 2α expression and activity in cancer., PMID:26974204

Sphingosine kinase 1 is required for TGF-β mediated fibroblastto- myofibroblast differentiation in ovarian cancer., PMID:26716409

Neutralizing S1P inhibits intratumoral hypoxia, induces vascular remodelling and sensitizes to chemotherapy in prostate cancer., PMID:25915662

Anti-S1P Antibody as a Novel Therapeutic Strategy for VEGFR TKI-Resistant Renal Cancer., PMID:25589614

Inhibition of SphK1 reduces radiation-induced migration and enhances sensitivity to cetuximab treatment by affecting the EGFR / SphK1 crosstalk., PMID:25245676

Oxidized LDL-induced angiogenesis involves sphingosine 1-phosphate: prevention by anti-S1P antibody., PMID:25176316

Role of sphingosine kinase 1 and sphingosine-1-phosphate in CD40 signaling and IgE class switching., PMID:25002116

Coupling between endocytosis and sphingosine kinase 1 recruitment., PMID:24929359

Osteoblast-derived sphingosine 1-phosphate to induce proliferation and confer resistance to therapeutics to bone metastasis-derived prostate cancer cells., PMID:24768038

E-Cadherin and EpCAM expression by NSCLC tumour cells associate with normal fibroblast activation through a pathway initiated by integrin αvβ6 and maintained through TGFβ signalling., PMID:24488011

Expression of macrophage markers in cryoglobulinemic glomerulonephritis - a possible role of CXCL9., PMID:24084359

Targeting the sphingosine kinase/sphingosine 1-phosphate pathway to treat chronic inflammatory kidney diseases., PMID:23789924

Sphingosine kinase 1 overexpression contributes to cetuximab resistance in human colorectal cancer models., PMID:23166225

Sphingosine kinase 1 is required for mesothelioma cell proliferation: role of histone acetylation., PMID:23028939

A specific sphingosine kinase 1 inhibitor attenuates airway hyperresponsiveness and inflammation in a mast cell-dependent murine model of allergic asthma., PMID:22939756

Inducible IL-33 expression by mast cells is regulated by a calcium-dependent pathway., PMID:22922818

The fate of renal allografts hinges on responses of the microvascular endothelium., PMID:22710034

Immunohistochemical detection of sphingosine-1-phosphate and sphingosine kinase-1 in human tissue samples., PMID:22528439

Sphingosine-kinase 1 and 2 contribute to oral sensitization and effector phase in a mouse model of food allergy., PMID:22020265

Sphingosine-1-phosphate acting via the S1P₁ receptor is a downstream signaling pathway in ceramide-induced hyperalgesia., PMID:21605625

Photolysis of caged sphingosine-1-phosphate induces barrier enhancement and intracellular activation of lung endothelial cell signaling pathways., PMID:21478254

Intracellular S1P generation is essential for S1P-induced motility of human lung endothelial cells: role of sphingosine kinase 1 and S1P lyase., PMID:21304987

S1P3-mediated cardiac fibrosis in sphingosine kinase 1 transgenic mice involves reactive oxygen species., PMID:19755413

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