Catalog No.
YXX54001
Expression system
E. coli
Species
Baker's yeast
Protein length
Met1-Phe355
Predicted molecular weight
55.89 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P04806
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Hexokinase-1, 2.7.1.1, Hexokinase PI, Hexokinase-A, HXK1, HKA, YFR053C
The role of hexokinases in epigenetic regulation: altered hexokinase expression and chromatin stability in yeast., PMID:39192292
Changing course: Glucose starvation drives nuclear accumulation of Hexokinase 2 in S. cerevisiae., PMID:37196001
Proteomic consequences of TDA1 deficiency in Saccharomyces cerevisiae: Protein kinase Tda1 is essential for Hxk1 and Hxk2 serine 15 phosphorylation., PMID:36302925
The Emi2 Protein of Saccharomyces cerevisiae is a Hexokinase Expressed under Glucose Limitation., PMID:34354536
Omics analysis reveals mechanism underlying metabolic oscillation during continuous very-high-gravity ethanol fermentation by Saccharomyces cerevisiae., PMID:33934328
Mig1 localization exhibits biphasic behavior which is controlled by both metabolic and regulatory roles of the sugar kinases., PMID:32948893
Glucose repression can be alleviated by reducing glucose phosphorylation rate in Saccharomyces cerevisiae., PMID:29422502
Co-transcriptional degradation by the 5'-3' exonuclease Rat1p mediates quality control of HXK1 mRNP biogenesis in S. cerevisiae., PMID:27124216
STP10 encodes a high-affinity monosaccharide transporter and is induced under low-glucose conditions in pollen tubes of Arabidopsis., PMID:26893494
Physiological and genomic characterisation of Saccharomyces cerevisiae hybrids with improved fermentation performance and mannoprotein release capacity., PMID:25879876
The gene YALI0E20207g from Yarrowia lipolytica encodes an N-acetylglucosamine kinase implicated in the regulated expression of the genes from the N-acetylglucosamine assimilatory pathway., PMID:25816199
N-acetylglucosamine kinase, HXK1 is involved in morphogenetic transition and metabolic gene expression in Candida albicans., PMID:23341961
Live-cell imaging of endogenous Ras-GTP shows predominant Ras activation at the plasma membrane and in the nucleus in Saccharomyces cerevisiae., PMID:23127800
Exploring the genetic control of glycolytic oscillations in Saccharomyces cerevisiae., PMID:22920924
Vectors for glucose-dependent protein expression in Saccharomyces cerevisiae., PMID:20949330
Glucose-free fructose production from Jerusalem artichoke using a recombinant inulinase-secreting Saccharomyces cerevisiae strain., PMID:20878538
Heterologous expression of yeast Hxt2 in Arabidopsis thaliana alters sugar uptake, carbon metabolism and gene expression leading to glucose tolerance of germinating seedlings., PMID:20101436
Gene loops function to maintain transcriptional memory through interaction with the nuclear pore complex., PMID:19933151
Puf1p acts in combination with other yeast Puf proteins to control mRNA stability., PMID:18094119
Glucose controls multiple processes in Saccharomyces cerevisiae through diverse combinations of signaling pathways., PMID:17428308
Different signalling pathways mediate glucose induction of SUC2, HXT1 and pyruvate decarboxylase in yeast., PMID:17311583
Nuclear pore association confers optimal expression levels for an inducible yeast gene., PMID:16760983
Nup-PI: the nucleopore-promoter interaction of genes in yeast., PMID:16455493
Expression of the cry11A gene of Bacillus thuringiensis ssp. israelensis in Saccharomyces cerevisiae., PMID:16091775
Saccharomyces cerevisiae engineered for xylose metabolism exhibits a respiratory response., PMID:15528549
Calorimetric determination of thermodynamic parameters of reaction reveals different enthalpic compensations of the yeast hexokinase isozymes., PMID:12611889
Mediator factor Med8p interacts with the hexokinase 2: implication in the glucose signalling pathway of Saccharomyces cerevisiae., PMID:12054864
The hexokinase 2 protein regulates the expression of the GLK1, HXK1 and HXK2 genes of Saccharomyces cerevisiae., PMID:11311123
The inducible N-acetylglucosamine catabolic pathway gene cluster in Candida albicans: discrete N-acetylglucosamine-inducible factors interact at the promoter of NAG1., PMID:11114181
Hexokinase regulates kinetics of glucose transport and expression of genes encoding hexose transporters in Saccharomyces cerevisiae., PMID:11073928
Metabolic signals trigger glucose-induced inactivation of maltose permease in Saccharomyces., PMID:10633097
Molecular cloning and characterization of the gene HXK1 encoding the hexokinase from Yarrowia lipolytica., PMID:10572255
Structure-function analysis of yeast hexokinase: structural requirements for triggering cAMP signalling and catabolite repression., PMID:10493925
A mutation Ser213/Asn in the hexokinase 1 from Schizosaccharomyces pombe increases its affinity for glucose., PMID:9790975
During the initiation of fermentation overexpression of hexokinase PII in yeast transiently causes a similar deregulation of glycolysis as deletion of Tps1., PMID:9580251
Application of mRNA differential display to investigate gene expression in thermotolerant cells of Saccharomyces cerevisiae., PMID:9559551
Msn2p and Msn4p control a large number of genes induced at the diauxic transition which are repressed by cyclic AMP in Saccharomyces cerevisiae., PMID:9495741
Differential requirement of the yeast sugar kinases for sugar sensing in establishing the catabolite-repressed state., PMID:8917466
Glucose repression may involve processes with different sugar kinase requirements., PMID:8755906
Multiple signalling pathways trigger the exquisite sensitivity of yeast gluconeogenic mRNAs to glucose., PMID:8793872
Analysis of a 36.2 kb DNA sequence including the right telomere of chromosome VI from Saccharomyces cerevisiae., PMID:8686379
The hexokinase gene is required for transcriptional regulation of the glucose transporter gene RAG1 in Kluyveromyces lactis., PMID:8321195
Analysis of transcription and translation of glycolytic enzymes in glucose-limited continuous cultures of Saccharomyces cerevisiae., PMID:1487726
Saccharomyces cerevisiae null mutants in glucose phosphorylation: metabolism and invertase expression., PMID:1874414
Isolation and characterization of mutations in the HXK2 gene of Saccharomyces cerevisiae., PMID:2685571
The hexokinase isoenzyme PII of Saccharomyces cerevisiae ia a protein kinase., PMID:2559946
Genetics of yeast glucokinase., PMID:6357942
A carbon catabolite repression mutant of Saccharomyces cerevisiae with elevated hexokinase activity: evidence for regulatory control of hexokinase PII synthesis., PMID:7035837