Catalog No.
YXX19401
Expression system
E. coli
Species
Escherichia coli
Protein length
Arg22-Lys471
Predicted molecular weight
49.49 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P00634
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Alkaline phosphatase, APase, 3.1.3.1, phoA, b0383, JW0374
Prevalence, antimicrobial resistance, and genetic profile of Escherichia coli in retail chicken parts in Zagazig City, Egypt., PMID:40253952
Enhanced production of extracellular triacylglycerol lipase for bioplastic degradation by replacing signal peptide., PMID:40221049
Expression and Characterization of Alkaline Phosphatase from Cobetia amphilecti KMM 296 in Transiently Transformed Tobacco Leaves and Transgenic Calli., PMID:39771268
Constructing High-Yielding Serratia marcescens for (-)-α-Bisabolol Production Based on the Exogenous Haloarchaeal MVA Pathway and Endogenous Molecular Chaperones., PMID:39699554
A rapid on-site loop-mediated isothermal amplification technology as an early warning system for the detection of Shiga toxin-producing Escherichia coli in water., PMID:39109421
Signal peptide replacement of Ag43 enables an efficient bacterial cell surface display of receptor-binding domain of coronavirus., PMID:38781660
FlgI Is a Sec-Dependent Effector of Candidatus Liberibacter asiaticus That Can Be Blocked by Small Molecules Identified Using a Yeast Screen., PMID:38276775
Defining Membrane Protein Topology Using pho-lac Reporter Fusions., PMID:37930528
The role of glutathione in periplasmic redox homeostasis and oxidative protein folding in Escherichia coli., PMID:37413765
Study on the drug resistance and pathogenicity of Escherichia coli isolated from calf diarrhea and the distribution of virulence genes and antimicrobial resistance genes., PMID:36620061
LysX2 is a Mycobacterium tuberculosis membrane protein with an extracytoplasmic MprF-like domain., PMID:35365094
Overexpression of a "Candidatus Liberibacter Asiaticus" Effector Gene CaLasSDE115 Contributes to Early Colonization in Citrus sinensis., PMID:35265048
G6P-capturing molecules in the periplasm of Escherichia coli accelerate the shikimate pathway., PMID:35257866
The Phage T4 Antiholin RI Has a Cleavable Signal Peptide, Not a SAR Domain., PMID:34456892
Enzyme promiscuity in natural environments: alkaline phosphatase in the ocean., PMID:34050259
MALDI-TOF MS Based Typing for Rapid Screening of Multiple Antibiotic Resistance E. coli and Virulent Non-O157 Shiga Toxin-Producing E. coli Isolated from the Slaughterhouse Settings and Beef Carcasses., PMID:33920071
Development of multiplexing gene silencing system using conditionally induced polycistronic synthetic antisense RNAs in Escherichia coli., PMID:33845307
Effective Small Molecule Antibacterials from a Novel Anti-Protein Secretion Screen., PMID:33805695
A Reporter System for Fast Quantitative Monitoring of Type 3 Protein Secretion in Enteropathogenic E. coli., PMID:33202599
Genome-wide identification of the Sec-dependent secretory protease genes in Erwinia amylovora and analysis of their expression during infection of immature pear fruit., PMID:32893528
Boosting toxic protein biosynthesis: transient in vivo inactivation of engineered bacterial alkaline phosphatase., PMID:32811518
Cotranslational folding of alkaline phosphatase in the periplasm of Escherichia coli., PMID:32790204
Screening Antibody Libraries with Colony Assay Using scFv-Alkaline Phosphatase Fusion Proteins., PMID:32599779
Assessing Site-Specific Enhancements Imparted by Hyperpolarized Water in Folded and Unfolded Proteins by 2D HMQC NMR., PMID:32338002
A secretion biosensor for monitoring Sec-dependent protein export in Corynebacterium glutamicum., PMID:31964372
Soluble expression and purification of high-bioactivity recombinant human bone morphogenetic protein-2 by codon optimisation in Escherichia coli., PMID:31603219
A PhoA-STII Based Method for Efficient Extracellular Secretion and Purification of Fab from Escherichia coli., PMID:33654866
A general platform for efficient extracellular expression and purification of Fab from Escherichia coli., PMID:30887174
Cellular Biocatalysts Using Synthetic Genetic Circuits for Prolonged and Durable Enzymatic Activity., PMID:30848859
Quantifying dynamic mechanisms of auto-regulation in Escherichia coli with synthetic promoter in response to varying external phosphate levels., PMID:30765722
The YoaW signal peptide directs efficient secretion of different heterologous proteins fused to a StrepII-SUMO tag in Bacillus subtilis., PMID:30732606
The effects of overexpression of cytoplasmic chaperones on secretory production of hirudin-PA in E. coli., PMID:30708036
Investigating the stability of the SecA-SecYEG complex during protein translocation across the bacterial membrane., PMID:30602566
Secretory expression, immunoaffinity purification and metal-binding ability of recombinant metallothionein (ShMT) from freshwater crab Sinopotamon henanense., PMID:30472469
Efficient production of human zona pellucida-3 in a prokaryotic expression system., PMID:30341457
Fitting replacement of signal peptide for highly efficient expression of three penicillin G acylases in E. coli., PMID:29968036
Novel (p)ppGpp Binding and Metabolizing Proteins of Escherichia coli., PMID:29511080
A photo-cross-linking approach to monitor folding and assembly of newly synthesized proteins in a living cell., PMID:29158258
Cytoplasmic Localization of Sulfide:Quinone Oxidoreductase and Persulfide Dioxygenase of Cupriavidus pinatubonensis JMP134., PMID:28939597
Altered Regulation of the Diguanylate Cyclase YaiC Reduces Production of Type 1 Fimbriae in a Pst Mutant of Uropathogenic Escherichia coli CFT073., PMID:28924030
Affinity-Based Purification of Polyisocyanopeptide Bioconjugates., PMID:28846388
Defining Membrane Protein Topology Using pho-lac Reporter Fusions., PMID:28667608
Secretory expression of a novel human spermatozoa antigen in E. coli and its application to a protein chip., PMID:28600648
Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli., PMID:28497288
Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA., PMID:28350826
SEC-Translocon Dependent Extracytoplasmic Proteins of Candidatus Liberibacter asiaticus., PMID:28066334
A Positive Selection for Nucleoside Kinases in E. coli., PMID:27677184
Structural basis for the antifolding activity of a molecular chaperone., PMID:27501151
High purity recombinant human growth hormone (rhGH) expression in Escherichia coli under phoA promoter., PMID:27459425
Expression and Secretion of Endostar Protein by Escherichia Coli: Optimization of Culture Conditions Using the Response Surface Methodology., PMID:27377615
For research use only. Not for human or drug use.
