Catalog No.
EVV00344
Expression system
Mammalian Cells
Species
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Protein length
Arg316-Phe538
Predicted molecular weight
26.61 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
UFO69279.1
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
surface glycoprotein, S, RBD, Omicron, B.1.1.529, BA.1, Receptor-Binding Domain
Recombinant receptor-binding motif of spike COVID-19 vaccine candidate induces SARS-CoV-2 neutralizing antibody response., PMID:40256231
A rapid point-of-care population-scale dipstick assay to identify and differentiate SARS-CoV-2 variants in COVID-19-positive patients., PMID:39498137
Highly selective split intein method for efficient separation and purification of recombinant therapeutic proteins from mammalian cell culture fluid., PMID:39405639
A SARS-CoV-2 peptide antigen purified from bacteria and displayed in a high-density repetitive manner on a virus-like particle could generate anti-SARS-CoV-2 neutralizing antibodies unlike free peptide., PMID:39205338
Amyloidogenesis of SARS-CoV-2 delta plus and omicron variants receptor-binding domain (RBD): impact of SUMO fusion tag., PMID:39182215
A cell based assay using virus-like particles to screen AM type mimics for SARS-CoV-2 neutralisation., PMID:38735141
Flow Cytometry-Based Measurement of Antibodies Specific for Cell Surface-Expressed Folded SARS-CoV-2 Receptor-Binding Domains., PMID:38675759
Effective expression and characterization of the receptor binding domains in SARS-CoV-2 Spike proteins from original strain and variants of concern using Bombyx mori nucleopolyhedrovirus in silkworm., PMID:38395208
A Biosensor Assay Based on Coiled-Coil-Mediated Human ACE2 Receptor Capture for the Analysis of Its Interactions with the SARS-CoV-2 Receptor Binding Domain., PMID:38315361
Developing of SARS-CoV-2 fusion protein expressed in E. coli Shuffle T7 for enhanced ELISA detection sensitivity - an integrated experimental and bioinformatic approach., PMID:38234051
Impact of mutations on the plant-based production of recombinant SARS-CoV-2 RBDs., PMID:37868317
Recombinant rotavirus expressing the glycosylated S1 protein of SARS-CoV-2., PMID:37830788
Heterologous DNA-prime/protein-boost immunization with a monomeric SARS-CoV-2 spike antigen redundantizes the trimeric receptor-binding domain structure to induce neutralizing antibodies in old mice., PMID:37753087
Optimization of an alum-anchored clinical HIV vaccine candidate., PMID:37573422
Soluble SARS-CoV-2 RBD and human ACE2 peptidase domain produced in Drosophila S2 cells show functions evoking virus-cell interface., PMID:37405395
Development of an Effective Neutralizing Antibody Assay for SARS-CoV-2 Diagnosis., PMID:37333734
COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples., PMID:37284350
Efficient Purification of Polyhistidine-Tagged Recombinant Proteins Using Functionalized Corundum Particles., PMID:37218748
Broad immunity to SARS-CoV-2 variants of concern mediated by a SARS-CoV-2 receptor-binding domain protein vaccine., PMID:37148585
Efficient virus detection utilizing chitin-immobilized nanobodies synthesized in Ustilago maydis., PMID:36948402
SIM imaging resolves endocytosis of SARS-CoV-2 spike RBD in living cells., PMID:36889309
A novel heterologous receptor-binding domain dodecamer universal mRNA vaccine against SARS-CoV-2 variants., PMID:36647424
Thermophilic Filamentous Fungus C1-Cell-Cloned SARS-CoV-2-Spike-RBD-Subunit-Vaccine Adjuvanted with Aldydrogel®85 Protects K18-hACE2 Mice against Lethal Virus Challenge., PMID:36560529
A highly sensitive bead-based flow cytometric competitive binding assay to detect SARS-CoV-2 neutralizing antibody activity., PMID:36532082
Tag-Free SARS-CoV-2 Receptor Binding Domain (RBD), but Not C-Terminal Tagged SARS-CoV-2 RBD, Induces a Rapid and Potent Neutralizing Antibody Response., PMID:36366348
Utilization of Receptor-Binding Domain of SARS-CoV-2 Spike Protein Expressed in Escherichia coli for the Development of Neutralizing Antibody Assay., PMID:36103078
Optical Biosensor for the Detection of Infectious Diseases Using the Copolymer F8T2 with Application to COVID-19., PMID:35957230
Identification of three conserved linear B cell epitopes on the SARS-CoV-2 spike protein., PMID:35916768
Utilization of SUMO Tag and Freeze-thawing Method for a High-level Expression and Solubilization of Recombinant Human Angiotensinconverting Enzyme 2 (rhACE2) Protein in E. coli., PMID:35838231
Using Species a Rotavirus Reverse Genetics to Engineer Chimeric Viruses Expressing SARS-CoV-2 Spike Epitopes., PMID:35758692
Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron., PMID:35609088
Phosphate-regulated expression of the SARS-CoV-2 receptor-binding domain in the diatom Phaeodactylum tricornutum for pandemic diagnostics., PMID:35487958
Insect cell expression and purification of recombinant SARS-COV-2 spike proteins that demonstrate ACE2 binding., PMID:35481636
Addition of arginine hydrochloride and proline to the culture medium enhances recombinant protein expression in Brevibacillus choshinensis: The case of RBD of SARS-CoV-2 spike protein and its antibody., PMID:35231586
A Multi-Disulfide Receptor-Binding Domain (RBD) of the SARS-CoV-2 Spike Protein Expressed in E. coli Using a SEP-Tag Produces Antisera Interacting with the Mammalian Cell Expressed Spike (S1) Protein., PMID:35163624
SARS-CoV-2 receptor binding domain radio-probe: a non-invasive approach for angiotensin-converting enzyme 2 mapping in mice., PMID:34815544
Receptor-binding domain of SARS-CoV-2 spike protein efficiently inhibits SARS-CoV-2 infection and attachment to mouse lung., PMID:34671199
Affinity Tag Coating Enables Reliable Detection of Antigen-Specific B Cells in Immunospot Assays., PMID:34440612
The development of Nanosota-1 as anti-SARS-CoV-2 nanobody drug candidates., PMID:34338634
BNT162b2 mRNA SARS-CoV-2 Vaccine Elicits High Avidity and Neutralizing Antibodies in Healthcare Workers., PMID:34207300
Production of the Receptor-binding Domain of the Viral Spike Proteins from 2003 and 2019 SARS CoVs and the Four Common Human Coronaviruses for Serologic Assays and Inhibitor Screening., PMID:34150933
Single-component, self-assembling, protein nanoparticles presenting the receptor binding domain and stabilized spike as SARS-CoV-2 vaccine candidates., PMID:33741598
Serological Test to Determine Exposure to SARS-CoV-2: ELISA Based on the Receptor-Binding Domain of the Spike Protein (S-RBDN318-V510) Expressed in Escherichia coli., PMID:33578665
Isoelectric point determination by imaged CIEF of commercially available SARS-CoV-2 proteins and the hACE2 receptor., PMID:33533060
Novel Regeneration Approach for Creating Reusable FO-SPR Probes with NTA Surface Chemistry., PMID:33451032
Capsid-like particles decorated with the SARS-CoV-2 receptor-binding domain elicit strong virus neutralization activity., PMID:33436573
Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays., PMID:33248226
Development of a nano-luciferase based assay to measure the binding of SARS-CoV-2 spike receptor binding domain to ACE-2., PMID:33239166
Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays., PMID:33236017
The Development of a Novel Nanobody Therapeutic for SARS-CoV-2., PMID:33236012
For research use only. Not for human or drug use.
