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Anti-HSPA5 Polyclonal Antibody (PHC84902)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:P11021
Overview

Catalog No.

PHC84902

Species reactivity

Human, Mouse

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HSPA5 (Leu351-Leu654).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Heat shock protein 70 family protein 5, 78 kDa glucose-regulated protein, HSPA5, Binding-immunoglobulin protein, GRP-78, HSP70 family protein 5, Heat shock protein family A member 5, Endoplasmic reticulum chaperone BiP, GRP78, BiP, Immunoglobulin heavy chain-binding protein

Purification

Purified by antigen affinity column.

Accession

P11021

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with HSPA5 antibody (PHC84902) at 1 µg/ml.

    Lane 1: HepG2 cell lysate
    Lane 2: NIH3T3 cell lysate
    Lane 3: 4T1 cell lysate
    Lane 4: MCF-7 cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: PC-12 cell lysate
    Lane 7: Hela cell lysate
    Lane 8: A549 cell lysate
    Lane 9: Mouse brain lysate
    Lane 10: Mouse liver lysate
    Lane 11: Rat brain lysate
    Lane 12: Rat liver lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 72 kDa
    Observed MW: 70-78 kDa
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-HSPA5 Polyclonal Antibody [PHC84902]
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