Anti-Human CD31/PECAM1 Antibody (SAA2178) (RHD16402)

specific references (0) DATASHEET

Host species：Mouse

Isotype：VHH-mFc (IgG2a)

Applications：ELISA, WB

Accession：P16284

Overview

Catalog No.

RHD16402

Species reactivity

Human

Host species

Mouse

Isotype

VHH-mFc (IgG2a)

Clonality

Monoclonal

Target

Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P16284

Applications

ELISA, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA2178

Data Image

WESTERN BLOT

Various lysates were subjected to SDS PAGE followed by western blot with CD31 antibody (RHD16402) at 1μg/mL .

Lane 1: THP-1 cell lysate
Lane 2: Jurkat cell lysate

Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

Predict MW: 83 kDa
Observed MW: 130 kDa
Flow-cytometry

Flow-cytometry using anti-human CD31 antibody. Jurkat cells were stained with an irrelevant antibody (Green Histogram) or an anti-human CD31 antibody monoclonal antibody (Catalog # RHD16402 , Blue Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (AntibodySystem: PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD31 antibody. THP-1 cells were stained with an irrelevant antibody (Green Histogram) or an anti-human CD31 antibody monoclonal antibody (Catalog # RHD16402 , Blue Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (AntibodySystem: PMB96441) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Document Download

Anti-Human CD31/PECAM1 Antibody (SAA2178).pdf

$ 328

Product specifications

100 μg 328 1 mg 1640

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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