Catalog No.
YXX16101
Expression system
E. coli
Species
Lachnospiraceae bacterium ND2006
Protein length
Met19-Gly1246
Predicted molecular weight
162.16KDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
WP_051666128.1
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
type V CRISPR-associated protein Cas12a/Cpf1, cas12a
Kinetic analysis and engineering of thermostable Cas12a for nucleic acid detection., PMID:40512544
Purification of CRISPR Cas12a from E. coli cell lysates using peptide affinity ligands., PMID:40403646
Gene Editing and Protein Tagging in the Oomycete Phytophthora infestans Using CRISPR-Cas12a., PMID:39729268
Deciphering the Substrate Specificity Reveals that CRISPR-Cas12a Is a Bifunctional Enzyme with Both Endo- and Exonuclease Activities., PMID:38575054
Improved genome editing by an engineered CRISPR-Cas12a., PMID:36537251
New PAM Improves the Single-Base Specificity of crRNA-Guided LbCas12a Nuclease., PMID:36431062
Efficient target cleavage by Type V Cas12a effectors programmed with split CRISPR RNA., PMID:34951459
Multiplexed activation in mammalian cells using a split-intein CRISPR/Cas12a based synthetic transcription factor., PMID:34908140
A robust and practical CRISPR/crRNA screening system for soybean cultivar editing using LbCpf1 ribonucleoproteins., PMID:32945949
Multiplexed conditional genome editing with Cas12a in Drosophila., PMID:32843348
Structural insight into multistage inhibition of CRISPR-Cas12a by AcrVA4., PMID:31467167
A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants., PMID:28211909
For research use only. Not for human or drug use.
