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Anti-mCherry Monoclonal Antibody (1A186)

Catalog #:   MXX51302 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG2b
Applications: ELISA,IHC, IF, WB
Accession: X5DSL3
Overview

Catalog No.

MXX51302

Species reactivity

mCherry, DsRed

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Immunogen

mCherry

Tested applications

ELISA: 1:2000-1:6000, IHC: 1:20-1:200, IF: 1:20-1:200, WB: 1:1000-1:5000

Target

MCherry fluorescent protein, mCherry

Concentration

2 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

X5DSL3

Applications

ELISA,IHC, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

1A186

Data Image
  • WESTERN BLOT
    Various Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with mCherry Tag antibody (MXX51302) at 1μg/ml.

    Lane 1: Recombinant mCherry Protein(YXX51301)
    Lane 2: Recombinant DsRed2 Protein (YWK27401)

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

References

Live-cell imaging of a plant virus replicase during infection using a genetically encoded, antibody-based probe., PMID:40479561

Generation of iPSC lines with tagged α-synuclein for visualization of endogenous protein in human cellular models of neurodegenerative disorders., PMID:40456614

Development and characterization of a novel NEIL1 nanobody., PMID:40440929

Inactivation of SIAH-1 E3 ligase attenuates Aβ toxicity by suppressing ubiquitin-dependent DVE-1 degradation in Caenorhabditis elegans models of Alzheimer's disease., PMID:40349774

The commercial PRRSV attenuated vaccine can be a potentially effective live trivalent vaccine vector., PMID:40316839

Colorectal cancer stem cells develop NK cell resistance via homotypic cell-in-cell structures suppressed by Stathmin1., PMID:40225568

Generation and characterization of infectious clones of chikungunya virus from an Indian strain as a resource towards chikungunya vaccine research., PMID:40216162

Expanding the fluorescent toolkit: Blue fluorescent protein-expressing Plasmodium berghei for enhanced multiplex microscopy., PMID:40029851

Xmas-NT: A Novel Two-Color Fluorescence Micro Neutralization Test for the Detection of Neutralizing Antibodies Against Monkeypox Virus., PMID:39977402

Development and application of a high-titer VSV-based HCoV-NL63 pseudovirus system via C-terminal 14 amino acid truncation of spike., PMID:39922054

Enhancing Neuron Activity Promotes Functional Recovery by Inhibiting Microglia-Mediated Synapse Elimination After Stroke., PMID:39772780

Rapid Development of Modified Vaccinia Virus Ankara (MVA)-Based Vaccine Candidates Against Marburg Virus Suitable for Clinical Use in Humans., PMID:39771978

Expressing red fluorescent protein on the surface of Escherichia coli using C-terminal domain of autotransporters., PMID:39744513

Synthetic anti-RNA antibody derivatives for RNA visualization in mammalian cells., PMID:39739875

CKAP4 in hepatocellular carcinoma: competitive RETREG1/FAM134B binding, reticulophagy regulation, and cancer progression., PMID:39689859

Hypoxia favors tumor growth in colorectal cancer in an integrin αDβ1/hemoglobin δ-dependent manner., PMID:39626964

CpG-Adjuvanted Virus-like Particle Vaccine Induces Protective Immunity Against Leishmania donovani Infection., PMID:39447003

[Establishment of a humanized mouse model of HIV-1 infection and quantification of integrated HIV proviral DNA in vivo]., PMID:39044584

A Single-step Generation of AlissAID-based Conditional Knockdown Strains Using Nanobody that Targets GFP or mCherry in Budding Yeast., PMID:38948262

Coxiella burnetii-containing vacuoles interact with host recycling endosomal proteins Rab11a and Rab35 for vacuolar expansion and bacterial growth., PMID:38841112

Visualizing histone H4K20me1 in knock-in mice expressing the mCherry-tagged modification-specific intracellular antibody., PMID:38762823

Affinity targeting of therapeutic proteins to the bone surface-local delivery of sclerostin-neutralizing antibody enhances efficacy., PMID:38526976

Quantitative and Standardized Pseudovirus Neutralization Assay for COVID-19., PMID:38526789

IL7 increases targeted lipid nanoparticle-mediated mRNA expression in T cells in vitro and in vivo by enhancing T cell protein translation., PMID:38513098

Intracellular cholesterol visualization in brain tissue using D4H∗., PMID:38100357

Characterisation of a Live-Attenuated Rabies Virus Expressing a Secreted scFv for the Treatment of Rabies., PMID:37632016

Characterising the structural and cellular role of immunoglobulin C-terminal lysine in secretory pathways., PMID:37495115

Cell-based BSEP trans-inhibition: A novel, non-invasive test for diagnosis of antibody-induced BSEP deficiency., PMID:37425215

Synthetic receptor platform to identify loss-of-function single nucleotide variants and designed mutants in the death receptor Fas/CD95., PMID:37392849

A simplified method for measuring neutralising antibodies against rabies virus., PMID:37391076

Development of AlissAID system targeting GFP or mCherry fusion protein., PMID:37315088

Evaluation of the genotype I Japanese encephalitis virus as a stable viral vector for foreign gene expression., PMID:37301446

Pathogenicity and immunogenicity of gI/gE/TK-gene-deleted Felid herpesvirus 1 variants in cats., PMID:37143065

Structural Insights into the Binding of Red Fluorescent Protein mCherry-Specific Nanobodies., PMID:37108116

Screening antivirals with a mCherry-expressing recombinant bovine respiratory syncytial virus: a proof of concept using cyclopamine., PMID:37069656

A bicellular fluorescent ductal carcinoma in situ (DCIS)-like tumoroid to study the progression of carcinoma: practical approaches and optimization., PMID:36946175

Antibiotic-induced degradation of antitoxin enhances the transcription of acetyltransferase-type toxin-antitoxin operon., PMID:36857516

Fitness of mCherry Reporter Tick-Borne Encephalitis Virus in Tick Experimental Models., PMID:36560677

Endotoxin-free gram-negative bacterium as a system for production and secretion of recombinant proteins., PMID:36445389

Nanobody derived using a peptide epitope from the spike protein receptor-binding motif inhibits entry of SARS-CoV-2 variants., PMID:36423687

Basal Cell-derived WNT7A Promotes Fibrogenesis at the Fibrotic Niche in Idiopathic Pulmonary Fibrosis., PMID:36318668

Antiviral Immunoglobulins of Chicken Egg Yolk for Potential Prevention of SARS-CoV-2 Infection., PMID:36298676

Nanobody-based RFP-dependent Cre recombinase for selective anterograde tracing in RFP-expressing transgenic animals., PMID:36114373

Amoeba-inspired magnetic microgel assembly assisted by engineered dextran-binding protein for vaccination against life-threatening systemic infection., PMID:36090612

PRPF31 interacts with PRPH2 confirmed by co-immunoprecipitation and co-localization., PMID:36088804

Establishment of a human cell line with a surface display system for screening and optimizing Na+-taurocholate cotransporting polypeptide-binding peptides., PMID:36060770

High-efficiency recombinant protein purification using mCherry and YFP nanobody affinity matrices., PMID:36040252

Monitoring SARS-CoV-2 Infection Using a Double Reporter-Expressing Virus., PMID:35980204

Generation, Characterization, and Applications of Influenza A Reporter Viruses., PMID:35821477

Rgs16 promotes antitumor CD8+ T cell exhaustion., PMID:35622904

Datasheet
$ 130
Product specifications
50 μg 130 100 μg 220

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For research use only. Not for human or drug use.

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Anti-mCherry Monoclonal Antibody (1A186) [MXX51302]
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