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Anti-Human ATP5PO Polyclonal Antibody (PHE54601)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:P48047
Overview

Catalog No.

PHE54601

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ATP5PO (Gly36-Val213).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

ATP synthase peripheral stalk subunit OSCP, ATP5O, Oligomycin sensitivity conferral protein, ATP5PO, OSCP, ATPO, ATP synthase subunit O, mitochondrial

Purification

Purified by antigen affinity column.

Accession

P48047

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with ATP5PO antibody (PHE54601) at 1 µg/ml.

    Lane 1: A549 cell lysate
    Lane 2: SKOV3 cell lysate
    Lane 3: MCF-7 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: HepG2 cell lysate
    Lane 6: 293T cell lysate
    Lane 7: Hela cell lysate
    Lane 8: Mouse brain lysate
    Lane 9: Mouse heart lysate
    Lane 10: Mouse liver lysate
    Lane 11: Rat brain lysate
    Lane 12: Rat heart lysate
    Lane 13: Rat liver lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 23 kDa
    Observed MW: 24 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ATP5PO antibody (PHE54601) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 22 kDa
    Observed MW: 22 kDa
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human ATP5PO Polyclonal Antibody [PHE54601]
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