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Anti-BVDV E2 glycoprotein Polyclonal Antibody

Catalog #:   PVV14702 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, IF, WB
Accession: AGC12054.1
Overview

Catalog No.

PVV14702

Species reactivity

Bovine viral diarrhea virus (BVDV) (Mucosal disease virus)

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant BVDV (Mucosal disease virus) E2 glycoprotein (Cys1-Asp218).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:200, IF: 1:50-1:200, WB: 1:500-1:4000

Target

Genome polyprotein, N-terminal protease, N-pro, 3.4.22.-, Autoprotease p20, Capsid protein C, E(rns) glycoprotein, gp44/48, Envelope glycoprotein E1, gp33, Envelope glycoprotein E2, gp55, Viroporin p7, Non-structural protein 2-3, Cysteine protease NS2, 3.4.22.-, Non-structural protein 2, Serine protease NS3, 3.4.21.113, 3.6.1.15, 3.6.4.13, Non-structural protein 3, Non-structural protein 4A, NS4A, Non-structural protein 4B, NS4B, Non-structural protein 5A, NS5A, RNA-directed RNA polymerase, 2.7.7.48, NS5B

Concentration

0.72 mg/ml

Purification

Purified by antigen affinity column.

Accession

AGC12054.1

Applications

ELISA, IHC, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with E2 glycoprotein antibody (PVV14702) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 27 kDa
    Observed MW: 27 kDa
References

Efficacy of a DNA vaccine encoding the E2 glycoprotein of bovine viral diarrhea virus 1 fused to mouse lysosome-associated membrane protein 1., PMID:39488135

Evaluation of BVDV E2 proteins based on recombinant baculovirus expression system production as diagnostic antigens and immunogens., PMID:39317297

Characterization of Membrane Topology and Retention Signal of Pestiviral Glycoprotein E1., PMID:34011544

Sequence analysis of the DA domain of glycoprotein E2 of pestiviruses isolated from beef cattle in Southern Brazil., PMID:33554289

Immunogenicity evaluation of recombinant Lactobacillus casei W56 expressing bovine viral diarrhea virus E2 protein in conjunction with cholera toxin B subunit as an adjuvant., PMID:33004035

Dendritic Cell Targeting of Bovine Viral Diarrhea Virus E2 Protein Expressed by Lactobacillus casei Effectively Induces Antigen-Specific Immune Responses via Oral Vaccination., PMID:31242608

Enhanced immune responses to E2 protein and DNA formulated with ISA 61 VG administered as a DNA prime-protein boost regimen against bovine viral diarrhea virus., PMID:30077483

The construction of recombinant Lactobacillus casei expressing BVDV E2 protein and its immune response in mice., PMID:29408654

Retention and topology of the bovine viral diarrhea virus glycoprotein E2., PMID:28874234

Selection and characterization of specific nanobody against bovine virus diarrhea virus (BVDV) E2 protein., PMID:28582444

Local innate responses and protective immunity after intradermal immunization with bovine viral diarrhea virus E2 protein formulated with a combination adjuvant in cattle., PMID:28527686

Bovine Viral Diarrhea Virus Infects Monocyte-Derived Bovine Dendritic Cells by an E2-Glycoprotein-Mediated Mechanism and Transiently Impairs Antigen Presentation., PMID:27529119

Preparation of chicken IgY against recombinant E2 protein of bovine viral diarrhea virus (BVDV) and development of ELISA and ICA for BVDV detection., PMID:27484991

Enhanced neutralising antibody response to bovine viral diarrhoea virus (BVDV) induced by DNA vaccination in calves., PMID:26079613

Expression of Bovine Viral Diarrhea Virus Envelope Glycoprotein E2 in Yeast Pichia pastoris and its Application to an ELISA for Detection of BVDV Neutralizing Antibodies in Cattle., PMID:25837831

The bovine viral diarrhea virus E2 protein formulated with a novel adjuvant induces strong, balanced immune responses and provides protection from viral challenge in cattle., PMID:25454860

In vivo delivery of bovine viral diahorrea virus, E2 protein using hollow mesoporous silica nanoparticles., PMID:24811899

A multiepitope fusion antigen elicits neutralizing antibodies against enterotoxigenic Escherichia coli and homologous bovine viral diarrhea virus in vitro., PMID:23697572

Development and evaluation of a replicon particle vaccine expressing the E2 glycoprotein of bovine viral diarrhea virus (BVDV) in cattle., PMID:23356714

Expression, purification and crystallization of the ectodomain of the envelope glycoprotein E2 from Bovine viral diarrhoea virus., PMID:23295482

Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants., PMID:23291101

Development and evaluation of a Luminex multiplex serology assay to detect antibodies to bovine herpes virus 1, parainfluenza 3 virus, bovine viral diarrhoea virus, and bovine respiratory syncytial virus, with comparison to existing ELISA detection methods., PMID:21277304

Characterization of a new chimeric marker vaccine candidate with a mutated antigenic E2-epitope., PMID:19892497

Novel BVDV-2 mutants as new candidates for modified-live vaccines., PMID:19875253

Development and validation of an ELISA for quantitation of bovine viral diarrhea virus antigen in the critical stages of vaccine production., PMID:19665482

Identification of a bovine viral diarrhea virus 2 isolated from cattle in China., PMID:19537915

DNA prime protein boost strategies protect cattle from bovine viral diarrhea virus type 2 challenge., PMID:18198376

Induction of virus-neutralizing antibodies by immunization with Rachiplusia nu per os infected with a recombinant baculovirus expressing the E2 glycoprotein of bovine viral diarrhea virus., PMID:17905447

Selection of mimotopes of Bovine Viral Diarrhoea Virus using a solid-phase peptide library., PMID:17825961

Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle., PMID:17727807

Compatibility of plasmids encoding bovine viral diarrhea virus type 1 and type 2 E2 in a single DNA vaccine formulation., PMID:17597267

Evidence for positive selection on the E2 gene of bovine viral diarrhoea virus type 1., PMID:17566858

Priming with DNA encoding E2 and boosting with E2 protein formulated with CpG oligodeoxynucleotides induces strong immune responses and protection from Bovine viral diarrhea virus in cattle., PMID:16963756

Immunogenicity of a bovine viral diarrhea virus E2-C3d fusion protein containing a bovine homolog of C3d., PMID:15978284

The surface glycoprotein E2 of bovine viral diarrhoea virus contains an intracellular localization signal., PMID:15105527

Fusion to C3d enhances the immunogenicity of the E2 glycoprotein of type 2 bovine viral diarrhea virus., PMID:14747527

Genetic and antigenic variability in bovine viral diarrhea virus (BVDV) isolates from Belgium., PMID:11955635

Cytokine adjuvancy of BVDV DNA vaccine enhances both humoral and cellular immune responses in mice., PMID:11457549

Mucosal disease induced in cattle persistently infected with bovine viral diarrhea virus by antigenically different cytopathic virus., PMID:11448036

[Expression and characterization of 2 bovine viral diarrhea virus structural proteins (]BVDV)]., PMID:11407016

Recombinant bovine adenovirus type 3 expressing bovine viral diarrhea virus glycoprotein E2 induces an immune response in cotton rats., PMID:11112498

Chimeric classical swine fever viruses containing envelope protein E(RNS) or E2 of bovine viral diarrhoea virus protect pigs against challenge with CSFV and induce a distinguishable antibody response., PMID:11027808

Presence of bovine viral diarrhea virus (BVDV) E2 glycoprotein in VSV recombinant particles and induction of neutralizing BVDV antibodies in mice., PMID:10989181

Co-administration of IL-2 enhances antigen-specific immune responses following vaccination with DNA encoding the glycoprotein E2 of bovine viral diarrhoea virus., PMID:10946143

Vaccination of cattle with a DNA plasmid encoding the bovine viral diarrhoea virus major glycoprotein E2., PMID:10567644

Recombinant adenoviruses expressing the E2 protein of bovine viral diarrhea virus induce humoral and cellular immune responses., PMID:10436933

Localization of pestiviral envelope proteins E(rns) and E2 at the cell surface and on isolated particles., PMID:10355762

Bovine herpes virus expressing envelope protein (E2) of bovine viral diarrhea virus as a vaccine candidate., PMID:10342291

An experimental multivalent bovine virus diarrhea virus E2 subunit vaccine and two experimental conventionally inactivated vaccines induce partial fetal protection in sheep., PMID:10217598

Induction of humoral and cellular immune responses in mice by a recombinant fowlpox virus expressing the E2 protein of bovine viral diarrhea virus., PMID:10077834

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-BVDV E2 glycoprotein Polyclonal Antibody [PVV14702]
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