Catalog No.
YHA36501
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Met1-Ser299
Predicted molecular weight
35.33 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
O14974
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
MYPT1, Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, MBS, PPP1R12A, Protein phosphatase myosin-binding subunit, Myosin phosphatase target subunit 1
Caspase-mediated cleavage of a scaffold protein, MPRIP, yields a truncated form that is involved in repetitive bleb formation., PMID:40344468
Effects of Rho inhibitors on membrane depolarization-induced contraction of male rat caudal arterial smooth muscle., PMID:40165590
Alanine mutation of the targeting subunit of the myosin phosphatase, MYPT1 at threonine 696 reduces cGMP responsiveness of mouse femoral arteries., PMID:39551336
Functional analysis of a novel nonsense PPP1R12A variant in a Chinese family with infantile epilepsy., PMID:39334371
Loss-of-Function Variant in PPP1R12A -Related Urogenital and/or Brain Malformation Syndrome: Expanded Phenotype of Sex Reversal., PMID:39257322
Insight in the (Phospho)proteome of Vascular Smooth Muscle Cells Derived From Patients With Abdominal Aortic Aneurysm Reveals Novel Disease Mechanisms., PMID:39206541
Molecular mechanism of bitter taste receptor agonist-mediated relaxation of airway smooth muscle., PMID:39037554
Glucose influences endometrial receptivity to embryo implantation through O-GlcNAcylation-mediated regulation of the cytoskeleton., PMID:39010841
Gallbladder dysfunction caused by MYPT1 ablation triggers cholestasis-induced hepatic fibrosis in mice., PMID:38934703
Myosin phosphatase targeting subunit1 controls localization and motility of Rab7-containing vesicles: Is myosin phosphatase a cytoplasmic dynein regulator?, PMID:38700016
Identification of pivotal genes and regulatory networks associated with atherosclerotic carotid artery stenosis based on comprehensive bioinformatics analysis and machine learning., PMID:38694921
Differential expression of PPP1R12A transcripts, including those harbouring alternatively spliced micro-exons, in placentae from complicated pregnancies., PMID:38615553
The temporal protein signature analyses of developing human deciduous molar tooth germ., PMID:38522031
Myosin Light Chain Phosphatase Plays an Important Role in Cardiac Fibrosis in a Model of Mineralocorticoid Receptor-Associated Hypertension., PMID:38420846
PPP1R12A is a recycling endosomal phosphatase that facilitates YAP activation., PMID:37957190
Identification of PP1c-PPP1R12A Substrates Using Kinase-Catalyzed Biotinylation to Identify Phosphatase Substrates., PMID:37810667
Phosphatases modified by LH signaling in ovarian follicles: testing their role in regulating the NPR2 guanylyl cyclase†., PMID:37774352
Transcriptome Profiling Based at Different Time Points after Hatching Deepened Our Understanding on Larval Growth and Development of Amphioctopus fangsiao., PMID:37623871
Phosphatases modified by LH signaling in ovarian follicles: testing their role in regulating the NPR2 guanylyl cyclase., PMID:37333193
MYPT1SMKO Mice Function as a Novel Spontaneous Age- and Hypertension-Dependent Animal Model of CSVD., PMID:36843141
SPECC1L binds the myosin phosphatase complex MYPT1/PP1β and can regulate its distribution between microtubules and filamentous actin., PMID:36634848
Persistent Müllerian duct syndrome associated with genetic defects in the regulatory subunit of myosin phosphatase., PMID:36331510
MYPT1-PP1β phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesis., PMID:36175407
Dynamic miRNA profile of host T cells during early hepatic stages of Schistosoma japonicum infection., PMID:36119054
MYPT1 inhibits the metastasis of renal clear cell carcinoma via the MAPK8/N-cadherin pathway., PMID:36106411
Complex Transcriptional Profiles of the PPP1R12A Gene in Cells of the Circulatory System as Revealed by In Silico Analysis and Reverse Transcription PCR., PMID:35954160
MYPT1 reduction is a pathogenic factor of erectile dysfunction., PMID:35879418
Myosin light chain phosphatase catalytic subunit dephosphorylates cardiac myosin via mechanisms dependent and independent of the MYPT regulatory subunits., PMID:35872014
MYPT1/PP1-Mediated EZH2 Dephosphorylation at S21 Promotes Epithelial-Mesenchymal Transition in Fibrosis through Control of Multiple Families of Genes., PMID:35293697
Shuxuetong injection and its peptides enhance angiogenesis after hindlimb ischemia by activating the MYPT1/LIMK1/Cofilin pathway., PMID:35248678
Tissue-specific expression of myosin phosphatase subunits and isoforms in smooth muscle of mice and humans., PMID:35107022
Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis., PMID:34880255
Senescent murine femoral arteries undergo vascular remodelling associated with accelerated stress-induced contractility and reactivity to nitric oxide., PMID:34665520
The ROCK inhibitor Y-27632 ameliorates blood-spinal cord barrier disruption by reducing tight junction protein degradation via the MYPT1-MLC2 pathway after spinal cord injury in rats., PMID:34634287
Identification and Validation of a PPP1R12A-Related Five-Gene Signature Associated With Metabolism to Predict the Prognosis of Patients With Prostate Cancer., PMID:34484299
MX2-mediated innate immunity against HIV-1 is regulated by serine phosphorylation., PMID:34282309
Upregulation of microRNA miR-141-3p and its prospective targets in endometrial carcinoma: a comprehensive study., PMID:34180758
12(S)-hydroxyheptadeca-5Z,8E,10E-trienoic acid (12-HHT) induces cell growth and improves barrier function through BLT2 interaction in intestinal epithelial Caco-2 cell cultures., PMID:34161796
MicroRNA-455-5p Contributes to Cholangiocarcinoma Growth and Mediates Galangin's Anti-Tumor Effects., PMID:34149934
The lysosomal Ragulator complex plays an essential role in leukocyte trafficking by activating myosin II., PMID:34099704
O-GlcNAc modification of MYPT1 modulates lysophosphatidic acid-induced cell contraction in fibroblasts., PMID:34019870
MYPT1, regulated by miR-19b-3p inhibits the progression of non-small cell lung cancer via inhibiting the activation of wnt/β-catenin signaling., PMID:33964297
Protein interactome of the Cancerous Inhibitor of protein phosphatase 2A (CIP2A) in Th17 cells., PMID:33817627
Novel circular RNA circSOBP governs amoeboid migration through the regulation of the miR-141-3p/MYPT1/p-MLC2 axis in prostate cancer., PMID:33784000
Integrated Proteomic and Phosphoproteomics Analysis of DKK3 Signaling Reveals Activated Kinase in the Most Aggressive Gallbladder Cancer., PMID:33670899
CircPPP1R12A promotes the progression of colon cancer through regulating CTNNB1 via sponging miR-375., PMID:33595945
Acute glucose influx-induced mitochondrial hyperpolarization inactivates myosin phosphatase as a novel mechanism of vascular smooth muscle contraction., PMID:33579894
Mice carrying a schizophrenia-associated mutation of the Arhgap10 gene are vulnerable to the effects of methamphetamine treatment on cognitive function: association with morphological abnormalities in striatal neurons., PMID:33482876
Haplotype-based, case-control study of myosin phosphatase target subunit 1 (PPP1R12A) gene and hypertensive disorders of pregnancy., PMID:33459569
For research use only. Not for human or drug use.
