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Catalog No.
KAD76501
Description
This assay employs the quantitative sandwich enzyme immunoassay technique. Romiplostim has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and anti-Romiplostim will be captured by immobilized Romiplostim. After washing away any unbound substances, a biotin-labeled Romiplostim is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Romiplostim bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Anti-Romiplostim concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
31.25 - 2,000 ng/mL
Sensitivity
17.08 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
848.7 |
206.8 |
46.8 |
888.7 |
225.1 |
53.9 |
|
Standard deviation |
24.6 |
9.6 |
4.3 |
34.7 |
13.0 |
2.8 |
|
CV (%) |
2.9 |
4.7 |
9.1 |
3.9 |
5.8 |
5.2 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
For research use only. Not for human or drug use.
