Anti-Dupilumab ELISA Kit (KAD62601)

Catalog No.

KAD62601

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Dupilumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and anti-Dupilumab will be captured by immobilized Dupilumab. After washing away any unbound substances, a biotin-labeled Dupilumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Dupilumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Applications

Used for the quantitative determination of Anti-Dupilumab concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

3.125 - 100 ng/mL

Sensitivity

1.32ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (ng/mL)	59.4	28.7	5.2	63.5	28.9	7.4
Standard deviation	5.5	1.9	0.5	9.4	2.7	1.0
CV (%)	9.3	6.7	8.9	14.7	9.4	12.9

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.

Alternative Names

CD124,REGN668,SAR231893, CAS: 1190264-60-8

Document Download

Anti-Dupilumab ELISA Kit.pdf