Anti-Ankyrin-G Antibody (N106/20) (RHG35101)

specific references (0) DATASHEET

Host species：Mouse

Isotype：IgG2a, kappa

Applications：IB, ICC, IF

Overview

Catalog No.

RHG35101

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Target

Ankyrin-3, ANK3, ANK-3, Ankyrin-G

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Applications

IB, ICC, IF

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

N106/20

Data Image

Immunocytochemistry/Immunofluorescence

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin G Monoclonal Antibody, Clone N106/20 (RHG35101). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin G Monoclonal Antibody (RHG35101) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin G Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin G Monoclonal Antibody, Clone N106/20 (RHG35101). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin G Monoclonal Antibody (RHG35101) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ankyrin G Antibody. (D) Composite.

References

Datasheet

Document Download

Anti-Ankyrin-G Antibody (N106/20).pdf

$ 328

Product specifications

100 μg 328 1 mg 1640

Contact Information

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Mail: support@antibodysystem.com

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For research use only. Not for human or drug use.

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