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Anti-IRF9 Polyclonal Antibody

Catalog #:   PHF84401 Specific References (100) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q00978
Overview

Catalog No.

PHF84401

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IRF9 (Met1-Val393).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Interferon regulatory factor 9,ISGF3G,IRF-9,ISGF-3 gamma,ISGF3 p48 subunit,Transcriptional regulator ISGF3 subunit gamma,IRF9,Interferon-stimulated gene factor 3 gamma,IFN-alpha-responsive transcription factor subunit

Purification

Purified by antigen affinity column.

Accession

Q00978

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with IRF9 antibody (PHF84401) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 46 kDa
    Observed MW: 46 kDa
References

A sensitive assay for measuring whole-blood responses to type I IFNs., PMID:39312669

A sensitive assay for measuring whole-blood responses to type I IFNs., PMID:39312669

Knockdown of miR-155 alleviates skin damage in rats with chronic spontaneous urticaria by modulating the JAK/STAT signaling pathway., PMID:38951930

Knockdown of miR-155 alleviates skin damage in rats with chronic spontaneous urticaria by modulating the JAK/STAT signaling pathway., PMID:38951930

Elevated unphosphorylated STAT1 and IRF9 in T and B cells of primary sjögren's syndrome: Novel biomarkers for disease activity and subsets., PMID:38788537

Elevated unphosphorylated STAT1 and IRF9 in T and B cells of primary sjögren's syndrome: Novel biomarkers for disease activity and subsets., PMID:38788537

Impact of the host immune response on the development of equine herpesvirus myeloencephalopathy in horses., PMID:38767608

Impact of the host immune response on the development of equine herpesvirus myeloencephalopathy in horses., PMID:38767608

Genetics and measles, mumps and rubella vaccine response in childhood and adolescence-A systematic review., PMID:38157324

Genetics and measles, mumps and rubella vaccine response in childhood and adolescence-A systematic review., PMID:38157324

ISGF3 and STAT2/IRF9 Control Basal and IFN-Induced Transcription through Genome-Wide Binding of Phosphorylated and Unphosphorylated Complexes to Common ISRE-Containing ISGs., PMID:38139463

ISGF3 and STAT2/IRF9 Control Basal and IFN-Induced Transcription through Genome-Wide Binding of Phosphorylated and Unphosphorylated Complexes to Common ISRE-Containing ISGs., PMID:38139463

Differential expression of Type I interferon and inflammatory genes in SARS-CoV-2-infected patients treated with monoclonal antibodies., PMID:37904704

Differential expression of Type I interferon and inflammatory genes in SARS-CoV-2-infected patients treated with monoclonal antibodies., PMID:37904704

Human inborn errors of immunity associated with IRF4., PMID:37809068

Human inborn errors of immunity associated with IRF4., PMID:37809068

Data supporting the roles of BAP1, STING, and IFN-β in ISGF3 activation in ccRCC., PMID:36426057

Data supporting the roles of BAP1, STING, and IFN-β in ISGF3 activation in ccRCC., PMID:36426057

Species-specific transcriptomic changes upon respiratory syncytial virus infection in cotton rats., PMID:36195733

Species-specific transcriptomic changes upon respiratory syncytial virus infection in cotton rats., PMID:36195733

The TRRAP transcription cofactor represses interferon-stimulated genes in colorectal cancer cells., PMID:35244540

The TRRAP transcription cofactor represses interferon-stimulated genes in colorectal cancer cells., PMID:35244540

Monoclonal antibody-mediated neutralization of SARS-CoV-2 in an IRF9-deficient child., PMID:34702736

Monoclonal antibody-mediated neutralization of SARS-CoV-2 in an IRF9-deficient child., PMID:34702736

Activation of plasmacytoid dendritic cells promotes AML-cell fratricide., PMID:33953842

Activation of plasmacytoid dendritic cells promotes AML-cell fratricide., PMID:33953842

Activation of an Effective Immune Response after Yellow Fever Vaccination Is Associated with the Genetic Background and Early Response of IFN-γ and CLEC5A., PMID:33445752

Activation of an Effective Immune Response after Yellow Fever Vaccination Is Associated with the Genetic Background and Early Response of IFN-γ and CLEC5A., PMID:33445752

Oxidative DNA Damage Accelerates Skin Inflammation in Pristane-Induced Lupus Model., PMID:33072095

Oxidative DNA Damage Accelerates Skin Inflammation in Pristane-Induced Lupus Model., PMID:33072095

The Measles Virus V Protein Binding Site to STAT2 Overlaps That of IRF9., PMID:32581091

The Measles Virus V Protein Binding Site to STAT2 Overlaps That of IRF9., PMID:32581091

Clinical Management and Therapeutic Strategies for the Thyroid-Associated Ophthalmopathy: Current and Future Perspectives., PMID:32567373

Clinical Management and Therapeutic Strategies for the Thyroid-Associated Ophthalmopathy: Current and Future Perspectives., PMID:32567373

Proteome Analysis Reveals Distinct Mitochondrial Functions Linked to Interferon Response Patterns in Activated CD4+ and CD8+ T Cells., PMID:31354474

Proteome Analysis Reveals Distinct Mitochondrial Functions Linked to Interferon Response Patterns in Activated CD4+ and CD8+ T Cells., PMID:31354474

Platelet factor 4 promotes rapid replication and propagation of Dengue and Japanese encephalitis viruses., PMID:30527622

Platelet factor 4 promotes rapid replication and propagation of Dengue and Japanese encephalitis viruses., PMID:30527622

Gene signatures related to HAI response following influenza A/H1N1 vaccine in older individuals., PMID:27441275

Gene signatures related to HAI response following influenza A/H1N1 vaccine in older individuals., PMID:27441275

Development of Th17-Associated Interstitial Kidney Inflammation in Lupus-Prone Mice Lacking the Gene Encoding STAT-1., PMID:26636548

Development of Th17-Associated Interstitial Kidney Inflammation in Lupus-Prone Mice Lacking the Gene Encoding STAT-1., PMID:26636548

Fucose-based PAMPs prime dendritic cells for follicular T helper cell polarization via DC-SIGN-dependent IL-27 production., PMID:25278262

Fucose-based PAMPs prime dendritic cells for follicular T helper cell polarization via DC-SIGN-dependent IL-27 production., PMID:25278262

Interferon regulatory factor 9 is an essential mediator of heart dysfunction and cell death following myocardial ischemia/reperfusion injury., PMID:25150882

Interferon regulatory factor 9 is an essential mediator of heart dysfunction and cell death following myocardial ischemia/reperfusion injury., PMID:25150882

IL6 sensitizes prostate cancer to the antiproliferative effect of IFNα2 through IRF9., PMID:23913484

IL6 sensitizes prostate cancer to the antiproliferative effect of IFNα2 through IRF9., PMID:23913484

Suppressor of cytokine signalling protein SOCS1 and UBP43 regulate the expression of type I interferon-stimulated genes in human microvascular endothelial cells infected with Rickettsia conorii., PMID:23558133

Suppressor of cytokine signalling protein SOCS1 and UBP43 regulate the expression of type I interferon-stimulated genes in human microvascular endothelial cells infected with Rickettsia conorii., PMID:23558133

Beta interferon-mediated activation of signal transducer and activator of transcription protein 1 interferes with Rickettsia conorii replication in human endothelial cells., PMID:21690236

Beta interferon-mediated activation of signal transducer and activator of transcription protein 1 interferes with Rickettsia conorii replication in human endothelial cells., PMID:21690236

IL28B inhibits hepatitis C virus replication through the JAK-STAT pathway., PMID:21147189

IL28B inhibits hepatitis C virus replication through the JAK-STAT pathway., PMID:21147189

Safety, Tolerability, and Immunogenicity of Interferons., PMID:27713294

Safety, Tolerability, and Immunogenicity of Interferons., PMID:27713294

Neutralizing type I IFN antibodies trigger an IFN-like response in endothelial cells., PMID:18390705

Neutralizing type I IFN antibodies trigger an IFN-like response in endothelial cells., PMID:18390705

IRF9 and STAT1 are required for IgG autoantibody production and B cell expression of TLR7 in mice., PMID:18340381

IRF9 and STAT1 are required for IgG autoantibody production and B cell expression of TLR7 in mice., PMID:18340381

Development of monoclonal antibodies against GBF1 and their use in studying its functions., PMID:16318580

Development of monoclonal antibodies against GBF1 and their use in studying its functions., PMID:16318580

Interferon-stimulated transcription and innate antiviral immunity require deacetylase activity and histone deacetylase 1., PMID:14645718

Interferon-stimulated transcription and innate antiviral immunity require deacetylase activity and histone deacetylase 1., PMID:14645718

Cells previously desensitized to type 1 interferons display different mechanisms of activation of stat-dependent gene expression from naïve cells., PMID:14600148

Cells previously desensitized to type 1 interferons display different mechanisms of activation of stat-dependent gene expression from naïve cells., PMID:14600148

Measles virus suppresses interferon-alpha signaling pathway: suppression of Jak1 phosphorylation and association of viral accessory proteins, C and V, with interferon-alpha receptor complex., PMID:12620806

Measles virus suppresses interferon-alpha signaling pathway: suppression of Jak1 phosphorylation and association of viral accessory proteins, C and V, with interferon-alpha receptor complex., PMID:12620806

CpG-DNA-induced IFN-alpha production involves p38 MAPK-dependent STAT1 phosphorylation in human plasmacytoid dendritic cell precursors., PMID:12429724

CpG-DNA-induced IFN-alpha production involves p38 MAPK-dependent STAT1 phosphorylation in human plasmacytoid dendritic cell precursors., PMID:12429724

Role of interferon-stimulated gene factor 3gamma and beta interferon in HLA class I enhancement in synovial fibroblasts upon infection with Chlamydia trachomatis., PMID:12379691

Role of interferon-stimulated gene factor 3gamma and beta interferon in HLA class I enhancement in synovial fibroblasts upon infection with Chlamydia trachomatis., PMID:12379691

Measles virus activates NF-kappa B and STAT transcription factors and production of IFN-alpha/beta and IL-6 in the human lung epithelial cell line A549., PMID:11882993

Measles virus activates NF-kappa B and STAT transcription factors and production of IFN-alpha/beta and IL-6 in the human lung epithelial cell line A549., PMID:11882993

Interferon regulatory factor-two restricts expression of interferon-stimulated genes to the endometrial stroma and glandular epithelium of the ovine uterus., PMID:11566724

Interferon regulatory factor-two restricts expression of interferon-stimulated genes to the endometrial stroma and glandular epithelium of the ovine uterus., PMID:11566724

The neutralization of type I IFN biologic actions by anti-IFNAR-2 monoclonal antibodies is not entirely due to inhibition of Jak-Stat tyrosine phosphorylation., PMID:11096454

The neutralization of type I IFN biologic actions by anti-IFNAR-2 monoclonal antibodies is not entirely due to inhibition of Jak-Stat tyrosine phosphorylation., PMID:11096454

Lactobacilli and Streptococci activate NF-kappa B and STAT signaling pathways in human macrophages., PMID:10725732

Lactobacilli and Streptococci activate NF-kappa B and STAT signaling pathways in human macrophages., PMID:10725732

Dynamic redistribution of STAT1 protein in IFN signaling visualized by GFP fusion proteins., PMID:10091593

Dynamic redistribution of STAT1 protein in IFN signaling visualized by GFP fusion proteins., PMID:10091593

Phospholipase C gamma 1 overexpression and activation induced by interferon beta in human T lymphocytes: an ISGF3-independent response., PMID:9325014

Phospholipase C gamma 1 overexpression and activation induced by interferon beta in human T lymphocytes: an ISGF3-independent response., PMID:9325014

Regulation of interferon-alpha responsiveness by the duration of Janus kinase activity., PMID:9268319

Regulation of interferon-alpha responsiveness by the duration of Janus kinase activity., PMID:9268319

Susceptibility to virus infection is determined by a Stat-mediated response to the autocrine effect of virus-induced type I interferon., PMID:9199872

Susceptibility to virus infection is determined by a Stat-mediated response to the autocrine effect of virus-induced type I interferon., PMID:9199872

A dominant negative mutant of an IFN regulatory factor family protein inhibits both type I and type II IFN-stimulated gene expression and antiproliferative activity of IFNs., PMID:8943426

A dominant negative mutant of an IFN regulatory factor family protein inhibits both type I and type II IFN-stimulated gene expression and antiproliferative activity of IFNs., PMID:8943426

Role of STAT2 in the alpha interferon signaling pathway., PMID:7532278

Role of STAT2 in the alpha interferon signaling pathway., PMID:7532278

Differential tyrosine phosphorylation of the IFNAR chain of the type I interferon receptor and of an associated surface protein in response to IFN-alpha and IFN-beta., PMID:7813427

Differential tyrosine phosphorylation of the IFNAR chain of the type I interferon receptor and of an associated surface protein in response to IFN-alpha and IFN-beta., PMID:7813427

Transcriptional induction of genes by IFN-beta in mouse cells is regulated by a transcription factor similar to human ISGF-3., PMID:7847880

Transcriptional induction of genes by IFN-beta in mouse cells is regulated by a transcription factor similar to human ISGF-3., PMID:7847880

Mutant cell lines unresponsive to alpha/beta and gamma interferon are defective in tyrosine phosphorylation of ISGF-3 alpha components., PMID:8114747

Mutant cell lines unresponsive to alpha/beta and gamma interferon are defective in tyrosine phosphorylation of ISGF-3 alpha components., PMID:8114747

Interferon gamma-induced transcription of the high-affinity Fc receptor for IgG requires assembly of a complex that includes the 91-kDa subunit of transcription factor ISGF3., PMID:8483949

Interferon gamma-induced transcription of the high-affinity Fc receptor for IgG requires assembly of a complex that includes the 91-kDa subunit of transcription factor ISGF3., PMID:8483949

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-IRF9 Polyclonal Antibody [PHF84401]
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