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Anti-LCN2 Polyclonal Antibody

Catalog #:   PHF70701 Specific References (100) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P80188
Overview

Catalog No.

PHF70701

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human LCN2 (Gln21-Gly198).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Lipocalin-2,Neutrophil gelatinase-associated lipocalin,Oncogene 24p3,NGAL,HNL,Siderocalin,LCN2,25 kDa alpha-2-microglobulin-related subunit of MMP-9,p25

Purification

Purified by antigen affinity column.

Accession

P80188

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with LCN2 antibody (PHF70701) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 23 kDa
    Observed MW: 23 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with LCN2 antibody (PHF70701) at 1 μg/ml.

    Lane 1: HT29
    Lane 2: PC-3

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 22 kDa
    Observed MW: 22 kDa
  • Immunohistochemical
    Immunohistochemical analysis of rat lung stained for LCN2 with PHF70701.
References

Dynamics of antimicrobial proteins' expression and their bactericidal activity in mouse milk., PMID:40345232

Dynamics of antimicrobial proteins' expression and their bactericidal activity in mouse milk., PMID:40345232

Chitinase 3-like protein 1 deficiency ameliorates drug-induced acute liver injury by inhibition of neutrophil recruitment through lipocalin-2., PMID:40196357

Chitinase 3-like protein 1 deficiency ameliorates drug-induced acute liver injury by inhibition of neutrophil recruitment through lipocalin-2., PMID:40196357

Antibody-free LC-HRMS/MS method for simultaneous quantification of NGAL, CRP and SAA in serum from sepsis patients., PMID:39900142

Antibody-free LC-HRMS/MS method for simultaneous quantification of NGAL, CRP and SAA in serum from sepsis patients., PMID:39900142

LCN2 blockade mitigating metabolic dysregulation and redefining appetite control in type 2 diabetes., PMID:39808380

LCN2 blockade mitigating metabolic dysregulation and redefining appetite control in type 2 diabetes., PMID:39808380

Deficiency of neutrophil gelatinase-associated lipocalin elicits a hemophilia-like bleeding and clotting disorder in mice., PMID:39693621

Deficiency of neutrophil gelatinase-associated lipocalin elicits a hemophilia-like bleeding and clotting disorder in mice., PMID:39693621

Serum Neutrophil Biomarkers to Predict Crohn's Disease Progression and Infliximab Treatment Outcomes., PMID:39648597

Serum Neutrophil Biomarkers to Predict Crohn's Disease Progression and Infliximab Treatment Outcomes., PMID:39648597

Lipocalin-2 Serum Levels in Rheumatoid Arthritis Patients Treated with Adalimumab and Its Correlation with Proinflammatory Factors., PMID:39403549

Lipocalin-2 Serum Levels in Rheumatoid Arthritis Patients Treated with Adalimumab and Its Correlation with Proinflammatory Factors., PMID:39403549

Lipocalin-2 drives neuropsychiatric and cutaneous disease in MRL/lpr mice., PMID:39399497

Lipocalin-2 drives neuropsychiatric and cutaneous disease in MRL/lpr mice., PMID:39399497

Microbial chaperonin 60 inhibits osteoclast differentiation by interfering with RANK/RANKL binding and overexpression of lipocalin2., PMID:39303785

Microbial chaperonin 60 inhibits osteoclast differentiation by interfering with RANK/RANKL binding and overexpression of lipocalin2., PMID:39303785

Lipocalin-2 as a fundamental protein in type 2 diabetes and periodontitis in mice., PMID:39189666

Lipocalin-2 as a fundamental protein in type 2 diabetes and periodontitis in mice., PMID:39189666

C/EBPδ Mediates Immunity to Renal Autoinflammatory Disorders in a Stage-specific Manner., PMID:39082925

C/EBPδ Mediates Immunity to Renal Autoinflammatory Disorders in a Stage-specific Manner., PMID:39082925

Associations between Microglia and Astrocytic Proteins and Tau Biomarkers across the Continuum of Alzheimer's Disease., PMID:39062786

Associations between Microglia and Astrocytic Proteins and Tau Biomarkers across the Continuum of Alzheimer's Disease., PMID:39062786

Detection strip of anti-NGAL antibody coupled with fluorescent microspheres-A novel tool for reliable and accurate prediction of renal injury., PMID:39038591

Detection strip of anti-NGAL antibody coupled with fluorescent microspheres-A novel tool for reliable and accurate prediction of renal injury., PMID:39038591

Intestinal mRNA expression profiles associated with mucosal healing in ustekinumab-treated Crohn's disease patients: bioinformatics analysis and prospective cohort validation., PMID:38926732

Intestinal mRNA expression profiles associated with mucosal healing in ustekinumab-treated Crohn's disease patients: bioinformatics analysis and prospective cohort validation., PMID:38926732

Identifying hub genes in response to ustekinumab and the impact of ustekinumab treatment on fibrosis in Crohn's disease., PMID:38840917

Identifying hub genes in response to ustekinumab and the impact of ustekinumab treatment on fibrosis in Crohn's disease., PMID:38840917

Spinal lipocalin 2 as a factor in the development of central post-stroke pain., PMID:38705557

Spinal lipocalin 2 as a factor in the development of central post-stroke pain., PMID:38705557

Characterization of cells expressing lipocalin-2 (LCN2) as a reporter., PMID:38696838

Characterization of cells expressing lipocalin-2 (LCN2) as a reporter., PMID:38696838

Disposable label-free electrochemical immunosensor based on gold nanoparticles-Prussian blue for neutrophil gelatinase-associated lipocalin detection in urine samples., PMID:38555767

Disposable label-free electrochemical immunosensor based on gold nanoparticles-Prussian blue for neutrophil gelatinase-associated lipocalin detection in urine samples., PMID:38555767

Noninvasive Markers of Inflammation and Protein Loss Augment Diagnosis of Pediatric Celiac Disease., PMID:38483287

Noninvasive Markers of Inflammation and Protein Loss Augment Diagnosis of Pediatric Celiac Disease., PMID:38483287

Neutrophil-Associated Proteins as Novel Biomarkers Elevated in Cerebrospinal Fluid of Patients With Neurosyphilis., PMID:38427829

Neutrophil-Associated Proteins as Novel Biomarkers Elevated in Cerebrospinal Fluid of Patients With Neurosyphilis., PMID:38427829

The Role of Serological Markers in the Prediction of Disease Course and Response to Therapy in Inflammatory Bowel Disease., PMID:38073917

The Role of Serological Markers in the Prediction of Disease Course and Response to Therapy in Inflammatory Bowel Disease., PMID:38073917

Identification of Diagnosis and Typological Characteristics Associated with Ferroptosis for Ulcerative Colitis via Bioinformatics and Machine Learning., PMID:37957905

Identification of Diagnosis and Typological Characteristics Associated with Ferroptosis for Ulcerative Colitis via Bioinformatics and Machine Learning., PMID:37957905

Lipocalin-2: a therapeutic target to overcome neurodegenerative diseases by regulating reactive astrogliosis., PMID:37779143

Lipocalin-2: a therapeutic target to overcome neurodegenerative diseases by regulating reactive astrogliosis., PMID:37779143

Transcriptome analysis of renal ischemia/reperfusion (I/R) injury in BAFF and BAFF-R deficient mice., PMID:37751458

Transcriptome analysis of renal ischemia/reperfusion (I/R) injury in BAFF and BAFF-R deficient mice., PMID:37751458

[Lipocalin 2 induces self-limited inhibition of osteoblast differentiation of mesenchymal stem cells]., PMID:37712270

[Lipocalin 2 induces self-limited inhibition of osteoblast differentiation of mesenchymal stem cells]., PMID:37712270

Up-regulation of LCN2 in the anterior cingulate cortex contributes to neural injury-induced chronic pain., PMID:37362002

Up-regulation of LCN2 in the anterior cingulate cortex contributes to neural injury-induced chronic pain., PMID:37362002

Socs3 expression in myeloid cells modulates the pathogenesis of dextran sulfate sodium (DSS)-induced colitis., PMID:37283760

Socs3 expression in myeloid cells modulates the pathogenesis of dextran sulfate sodium (DSS)-induced colitis., PMID:37283760

LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia., PMID:36973755

LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia., PMID:36973755

Protective Role for Smooth Muscle Cell Hepcidin in Abdominal Aortic Aneurysm., PMID:36951059

Protective Role for Smooth Muscle Cell Hepcidin in Abdominal Aortic Aneurysm., PMID:36951059

Lipocalin-2 Is a Key Regulator of Neuroinflammation in Secondary Traumatic and Ischemic Brain Injury., PMID:36508119

Lipocalin-2 Is a Key Regulator of Neuroinflammation in Secondary Traumatic and Ischemic Brain Injury., PMID:36508119

Lcn2 mediates adipocyte-muscle-tumor communication and hypothermia in pancreatic cancer cachexia., PMID:36243318

Lcn2 mediates adipocyte-muscle-tumor communication and hypothermia in pancreatic cancer cachexia., PMID:36243318

Increased LCN2 (lipocalin 2) in the RPE decreases autophagy and activates inflammasome-ferroptosis processes in a mouse model of dry AMD., PMID:35473441

Increased LCN2 (lipocalin 2) in the RPE decreases autophagy and activates inflammasome-ferroptosis processes in a mouse model of dry AMD., PMID:35473441

Glioma-derived exosomes hijack the blood-brain barrier to facilitate nanocapsule delivery via LCN2., PMID:35341902

Glioma-derived exosomes hijack the blood-brain barrier to facilitate nanocapsule delivery via LCN2., PMID:35341902

Principal Component Analysis to Differentiate Patients with Palmoplantar Pustulosis from Those with Palmoplantar Pustular Psoriasis., PMID:35221589

Principal Component Analysis to Differentiate Patients with Palmoplantar Pustulosis from Those with Palmoplantar Pustular Psoriasis., PMID:35221589

Interleukin-17RA blockade by brodalumab decreases inflammatory pathways in hidradenitis suppurativa skin and serum., PMID:35191018

Interleukin-17RA blockade by brodalumab decreases inflammatory pathways in hidradenitis suppurativa skin and serum., PMID:35191018

Astrocytic α2-Na+/K+ ATPase inhibition suppresses astrocyte reactivity and reduces neurodegeneration in a tauopathy mouse model., PMID:35171651

Astrocytic α2-Na+/K+ ATPase inhibition suppresses astrocyte reactivity and reduces neurodegeneration in a tauopathy mouse model., PMID:35171651

Secretory protein beta-lactoglobulin in cattle stable dust may contribute to the allergy-protective farm effect., PMID:35169442

Secretory protein beta-lactoglobulin in cattle stable dust may contribute to the allergy-protective farm effect., PMID:35169442

Lipocalin 2 Influences Bone and Muscle Phenotype in the MDX Mouse Model of Duchenne Muscular Dystrophy., PMID:35055145

Lipocalin 2 Influences Bone and Muscle Phenotype in the MDX Mouse Model of Duchenne Muscular Dystrophy., PMID:35055145

Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury., PMID:35031018

Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury., PMID:35031018

Structural plasticity in the loop region of engineered lipocalins with novel ligand specificities, so-called Anticalins., PMID:34988429

Structural plasticity in the loop region of engineered lipocalins with novel ligand specificities, so-called Anticalins., PMID:34988429

A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis., PMID:34921145

A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis., PMID:34921145

NGAL as a Potential Target in Tumor Microenvironment., PMID:34830212

NGAL as a Potential Target in Tumor Microenvironment., PMID:34830212

Neutrophil Gelatinase-Associated Lipocalin From Macrophages Plays a Critical Role in Renal Fibrosis Via the CCL5 (Chemokine Ligand 5)-Th2 Cells-IL4 (Interleukin 4) Pathway., PMID:34794340

Neutrophil Gelatinase-Associated Lipocalin From Macrophages Plays a Critical Role in Renal Fibrosis Via the CCL5 (Chemokine Ligand 5)-Th2 Cells-IL4 (Interleukin 4) Pathway., PMID:34794340

RTEC-intrinsic IL-17-driven inflammatory circuit amplifies antibody-induced glomerulonephritis and is constrained by Regnase-1., PMID:34236049

RTEC-intrinsic IL-17-driven inflammatory circuit amplifies antibody-induced glomerulonephritis and is constrained by Regnase-1., PMID:34236049

Lipocalin 2 expression promotes tumor progression and therapy resistance by inhibiting ferroptosis in colorectal cancer., PMID:34146401

Lipocalin 2 expression promotes tumor progression and therapy resistance by inhibiting ferroptosis in colorectal cancer., PMID:34146401

Overexpression of Lipocalin-2 Inhibits Proliferation and Invasiveness of Human Glioblastoma Multiforme Cells by Activating ERK Targeting Cathepsin D Expression., PMID:34062746

Overexpression of Lipocalin-2 Inhibits Proliferation and Invasiveness of Human Glioblastoma Multiforme Cells by Activating ERK Targeting Cathepsin D Expression., PMID:34062746

Bovine Holo-Beta-Lactoglobulin Cross-Protects Against Pollen Allergies in an Innate Manner in BALB/c Mice: Potential Model for the Farm Effect., PMID:33746954

Bovine Holo-Beta-Lactoglobulin Cross-Protects Against Pollen Allergies in an Innate Manner in BALB/c Mice: Potential Model for the Farm Effect., PMID:33746954

Lipocalin-2 in Diabetic Complications of the Nervous System: Physiology, Pathology, and Beyond., PMID:33613327

Lipocalin-2 in Diabetic Complications of the Nervous System: Physiology, Pathology, and Beyond., PMID:33613327

Ranolazine alleviates contrast-associated acute kidney injury through modulation of calcium independent oxidative stress and apoptosis., PMID:33352171

Ranolazine alleviates contrast-associated acute kidney injury through modulation of calcium independent oxidative stress and apoptosis., PMID:33352171

An immune-based biomarker signature is associated with mortality in COVID-19 patients., PMID:33232303

An immune-based biomarker signature is associated with mortality in COVID-19 patients., PMID:33232303

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-LCN2 Polyclonal Antibody [PHF70701]
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