Catalog No.
PHF59301
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human SUMO1 (Met1-Val101).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
SUMO-1,SMT3C,SMT3H3,Sentrin,GMP1,Ubiquitin-like protein UBL1,UBL1,Small ubiquitin-related modifier 1,Ubiquitin-homology domain protein PIC1,SUMO1,Ubiquitin-like protein SMT3C,SMT3 homolog 3,Smt3C,GAP-modifying protein 1
Purification
Purified by antigen affinity column.
Accession
P63165
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
TRIM28 functions as SUMO ligase to SUMOylate TRAF6 and regulate NF-κB activation in HBV-replicating cells., PMID:39920527
Dysregulated gene expression of SUMO machinery components induces the resistance to anti-PD-1 immunotherapy in lung cancer by upregulating the death of peripheral blood lymphocytes., PMID:39211047
NrCAM activates the NF-κB signalling pathway by competitively binding to SUMO-1 and promotes Th17 cell differentiation in Graves' disease., PMID:39155774
UBE2I regulates the nuclear translocation of hnRNPA2B1 by contributing to SUMO modification in osteoarthritis., PMID:38955308
Site-specific identification and quantitation of endogenous SUMOylation based on SUMO-specific protease and strong anion exchange chromatography., PMID:38865749
Juvenile dermatomyositis with Anti-SAE antibodies in a Moroccan child associated with pseudo-angioedema: a case report., PMID:38773611
SUMOylation of annexin A6 retards cell migration and tumor growth by suppressing RHOU/AKT1-involved EMT in hepatocellular carcinoma., PMID:38566133
Development and characterization of monoclonal antibodies against p37 protein of African swine fever virus., PMID:38458287
A highly sensitive nanochannel device for the detection of SUMO1 peptides., PMID:37564410
SUMOylation of AnxA6 facilitates EGFR-PKCα complex formation to suppress epithelial cancer growth., PMID:37528485
Monoclonal antibody-based systematic identification of SUMO1-modification sites reveals TFII-I SUMOylation is involved in tumor growth., PMID:37450667
SUMO monoclonal antibodies vary in sensitivity, specificity, and ability to detect types of SUMO conjugate., PMID:36494414
Antibody-free enrichment method for proteome-wide analysis of endogenous SUMOylation sites., PMID:33736815
CGG expansion in NOTCH2NLC is associated with oculopharyngodistal myopathy with neurological manifestations., PMID:33239111
SAE1 promotes human glioma progression through activating AKT SUMOylation-mediated signaling pathways., PMID:31345225
Inhibition of Ubc9-Induced CRMP2 SUMOylation Disrupts Glioblastoma Cell Proliferation., PMID:31267313
SUMOylation of PCNA by PIAS1 and PIAS4 promotes template switch in the chicken and human B cell lines., PMID:30487218
Response: Commentary: Analysis of SUMO1-conjugation at synapses., PMID:29766991
Generation of specific inhibitors of SUMO-1- and SUMO-2/3-mediated protein-protein interactions using Affimer (Adhiron) technology., PMID:29138295
SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells., PMID:29038616
Proteome-wide Mapping of Endogenous SUMOylation Sites in Mouse Testis., PMID:28289178
Neuron-specific SUMO knockdown suppresses global gene expression response and worsens functional outcome after transient forebrain ischemia in mice., PMID:27919694
Systematic Localization and Identification of SUMOylation Substrates in Knock-In Mice Expressing Affinity-Tagged SUMO1., PMID:27631813
Detection of Protein SUMOylation In Situ by Proximity Ligation Assays., PMID:27631803
Real-Time Surface Plasmon Resonance (SPR) for the Analysis of Interactions Between SUMO Traps and Mono- or PolySUMO Moieties., PMID:27631800
PPARγ Regulates Mouse Meibocyte Differentiation and Lipid Synthesis., PMID:27531629
ON 01910.Na inhibits growth of diffuse large B-cell lymphoma by cytoplasmic sequestration of sumoylated C-MYB/TRAF6 complex., PMID:27150054
Transcription Factor hDREF Is a Novel SUMO E3 Ligase of Mi2α., PMID:27068747
Screening and identification of SUMP-proteins in sub-acute treatment with diazinon., PMID:26877855
A PTEN translational isoform has PTEN-like activity., PMID:26543340
A Comprehensive Overview on Myositis-Specific Antibodies: New and Old Biomarkers in Idiopathic Inflammatory Myopathy., PMID:26424665
HIF-1α SUMOylation affects the stability and transcriptional activity of HIF-1α in human lens epithelial cells., PMID:25877955
ATL response to arsenic/interferon therapy is triggered by SUMO/PML/RNF4-dependent Tax degradation., PMID:25395419
Study of MDM2 and SUMO-1 expression in actinic cheilitis and lip cancer., PMID:25241153
SUMO proteomics to decipher the SUMO-modified proteome regulated by various diseases., PMID:25236368
SUMO-1 plays crucial roles for spindle organization, chromosome congression, and chromosome segregation during mouse oocyte meiotic maturation., PMID:25123474
Neuron-specific Sumo1-3 knockdown in mice impairs episodic and fear memories., PMID:24690371
Identification and analysis of endogenous SUMO1 and SUMO2/3 targets in mammalian cells and tissues using monoclonal antibodies., PMID:24651501
SUMO-conjugating enzyme UBC9 promotes proliferation and migration of fibroblast-like synoviocytes in rheumatoid arthritis., PMID:24531852
A monoclonal antibody against the human SUMO-1 protein obtained by immunization with recombinant protein and CpG-DNA-liposome complex., PMID:24111868
Low-molecular-mass secretome profiling identifies C-C motif chemokine 5 as a potential plasma biomarker and therapeutic target for nasopharyngeal carcinoma., PMID:24080422
Evidence for multiple forms and modifications of human POT1., PMID:24054699
In vivo characterization of the properties of SUMO1-specific monobodies., PMID:24040933
Akt SUMOylation regulates cell proliferation and tumorigenesis., PMID:23884910
[Potential involvement of abnormal increased SUMO-1 in modulation of the formation of Alzheimer's disease senile plaques and neuritic dystrophy in APP/PS1 transgenic mice]., PMID:23788181
SUMO1 negatively regulates the transcriptional activity of EVI1 and significantly increases its co-localization with EVI1 after treatment with arsenic trioxide., PMID:23770046
Detecting endogenous SUMO targets in mammalian cells and tissues., PMID:23503365
Evaluation of the expression of p53, MDM2, and SUMO-1 in oral lichen planus., PMID:23350884
SUMO-1 is associated with a subset of lysosomes in glial protein aggregate diseases., PMID:23229893
SUMO1 modification of PTEN regulates tumorigenesis by controlling its association with the plasma membrane., PMID:22713753
For research use only. Not for human or drug use.
