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Anti-AIF1/IBA1 Polyclonal Antibody (PHF07401)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:P55008
Overview

Catalog No.

PHF07401

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human AIF1/IBA1 (Met1-Pro147).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Ionized calcium-binding adapter molecule 1,AIF1,AIF-1,Allograft inflammatory factor 1,IBA1,G1,Protein G1

Purification

Purified by antigen affinity column.

Accession

P55008

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with AIF1/IBA1 antibody (PHF07401) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 19 kDa
    Observed MW: 19 kDa
  • Immunocytochemistry/ Immunofluorescence
    AIF1 / IBA1 in U251 Cell Line.
    The U251 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with AIF1 / IBA1 (PHF07401) at 5.8 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-AIF1/IBA1 Polyclonal Antibody [PHF07401]
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