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Anti-Human CD32/FcRII/Ly-17 Polyclonal Antibody

Catalog #:   PHK22801 Specific References (100) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P08101
Overview

Catalog No.

PHK22801

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD32/FcRII/Ly-17 (Lys35-Gly202).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Lymphocyte antigen 17, Ly-17, CD32, Fcgr2, Fcgr2b, Ly-17

Purification

Purified by antigen affinity column.

Accession

P08101

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CD32/FcRII/Ly-17 antibody (PHK22801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 21 kDa
    Observed MW: 21 kDa
References

A proteogenomic tool uncovers protein markers for human microglial states., PMID:40236015

A proteogenomic tool uncovers protein markers for human microglial states., PMID:40236015

FcγRIIB (CD32B) antibodies enhance immune responses through activating FcγRs., PMID:40089806

FcγRIIB (CD32B) antibodies enhance immune responses through activating FcγRs., PMID:40089806

The CD64/CD28/CD3ζ chimeric receptor reprograms T-cell metabolism and promotes T-cell persistence and immune functions while triggering antibody-independent and antibody-dependent cytotoxicity., PMID:39962623

The CD64/CD28/CD3ζ chimeric receptor reprograms T-cell metabolism and promotes T-cell persistence and immune functions while triggering antibody-independent and antibody-dependent cytotoxicity., PMID:39962623

Antibody-Dependent Cellular Cytotoxicity of iPS Cell-Derived Natural Killer T Cells by Anti-GD2 mAb for Neuroblastoma., PMID:39916425

Antibody-Dependent Cellular Cytotoxicity of iPS Cell-Derived Natural Killer T Cells by Anti-GD2 mAb for Neuroblastoma., PMID:39916425

C-reactive protein promotes diabetic kidney disease via Smad3-mediated NLRP3 inflammasome activation., PMID:39539016

C-reactive protein promotes diabetic kidney disease via Smad3-mediated NLRP3 inflammasome activation., PMID:39539016

Characterising how a single bout of exercise in people with myeloma affects clonal plasma cell and immune effector cell frequency in blood, and daratumumab efficacy in vitro., PMID:39411424

Characterising how a single bout of exercise in people with myeloma affects clonal plasma cell and immune effector cell frequency in blood, and daratumumab efficacy in vitro., PMID:39411424

Autoreactive B cells remain active despite clinical disease control in rheumatoid arthritis., PMID:39342827

Autoreactive B cells remain active despite clinical disease control in rheumatoid arthritis., PMID:39342827

Systematic analysis of Fc mutations designed to enhance binding to Fc-gamma receptors., PMID:39306747

Systematic analysis of Fc mutations designed to enhance binding to Fc-gamma receptors., PMID:39306747

Systematic analysis of Fc mutations designed to reduce binding to Fc-gamma receptors., PMID:39279104

Systematic analysis of Fc mutations designed to reduce binding to Fc-gamma receptors., PMID:39279104

IL-8 Instructs Macrophage Identity in Lateral Ventricle Contacting Glioblastoma., PMID:38585888

IL-8 Instructs Macrophage Identity in Lateral Ventricle Contacting Glioblastoma., PMID:38585888

Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells., PMID:38579727

Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells., PMID:38579727

Control of complement-induced inflammatory responses to SARS-CoV-2 infection by anti-SARS-CoV-2 antibodies., PMID:38418557

Control of complement-induced inflammatory responses to SARS-CoV-2 infection by anti-SARS-CoV-2 antibodies., PMID:38418557

Trypanosoma brucei Invariant Surface Glycoprotein 75 Is an Immunoglobulin Fc Receptor Inhibiting Complement Activation and Antibody-Mediated Cellular Phagocytosis., PMID:38391367

Trypanosoma brucei Invariant Surface Glycoprotein 75 Is an Immunoglobulin Fc Receptor Inhibiting Complement Activation and Antibody-Mediated Cellular Phagocytosis., PMID:38391367

A Bispecific, Tetravalent Antibody Targeting Inflammatory and Pruritogenic Pathways in Atopic Dermatitis., PMID:38375189

A Bispecific, Tetravalent Antibody Targeting Inflammatory and Pruritogenic Pathways in Atopic Dermatitis., PMID:38375189

Type 2-polarized memory B cells hold allergen-specific IgE memory., PMID:38324637

Type 2-polarized memory B cells hold allergen-specific IgE memory., PMID:38324637

Anti-Vα24Jα18 TCR Antibody Tunes iNKT Cell Responses to Target and Kill CD1d-negative Tumors in an FcγRII (CD32)-dependent Manner., PMID:38319156

Anti-Vα24Jα18 TCR Antibody Tunes iNKT Cell Responses to Target and Kill CD1d-negative Tumors in an FcγRII (CD32)-dependent Manner., PMID:38319156

Fc-gamma receptor expression and cytokine responses to intravenous human immunoglobulin in whole blood from non-pregnant and pregnant women and newborns., PMID:38180331

Fc-gamma receptor expression and cytokine responses to intravenous human immunoglobulin in whole blood from non-pregnant and pregnant women and newborns., PMID:38180331

Nasal polyp antibody-secreting cells display proliferation signature in aspirin-exacerbated respiratory disease., PMID:37898408

Nasal polyp antibody-secreting cells display proliferation signature in aspirin-exacerbated respiratory disease., PMID:37898408

High levels of PD-L1 on platelets of NSCLC patients contributes to the pharmacological activity of Atezolizumab., PMID:37857253

High levels of PD-L1 on platelets of NSCLC patients contributes to the pharmacological activity of Atezolizumab., PMID:37857253

Developmental adaptations of γδ T cells and B cells in blood and intestinal mucosa from birth until weaning in Holstein bull calves., PMID:37806632

Developmental adaptations of γδ T cells and B cells in blood and intestinal mucosa from birth until weaning in Holstein bull calves., PMID:37806632

Macrophage N-glycan processing inhibits antibody-dependent cellular phagocytosis., PMID:37792857

Macrophage N-glycan processing inhibits antibody-dependent cellular phagocytosis., PMID:37792857

Function-structure approach reveals novel insights on the interplay of Immunoglobulin G 1 proteoforms and Fc gamma receptor IIa allotypes., PMID:37790943

Function-structure approach reveals novel insights on the interplay of Immunoglobulin G 1 proteoforms and Fc gamma receptor IIa allotypes., PMID:37790943

DENV-specific IgA contributes protective and non-pathologic function during antibody-dependent enhancement of DENV infection., PMID:37639455

DENV-specific IgA contributes protective and non-pathologic function during antibody-dependent enhancement of DENV infection., PMID:37639455

Role of C-Reactive Protein in Kidney Diseases., PMID:37065607

Role of C-Reactive Protein in Kidney Diseases., PMID:37065607

A 16-color full spectrum flow cytometric analysis for comprehensive evaluation of T-cell reconstitution in SIV-infected rhesus macaques., PMID:36496008

A 16-color full spectrum flow cytometric analysis for comprehensive evaluation of T-cell reconstitution in SIV-infected rhesus macaques., PMID:36496008

Direct Binding of Bovine IgG-Containing Immune Complexes to Human Monocytes and Their Putative Role in Innate Immune Training., PMID:36364714

Direct Binding of Bovine IgG-Containing Immune Complexes to Human Monocytes and Their Putative Role in Innate Immune Training., PMID:36364714

Immunomodulatory effects of β-defensin 2 on macrophages induced immuno-upregulation and their antitumor function in breast cancer., PMID:36324077

Immunomodulatory effects of β-defensin 2 on macrophages induced immuno-upregulation and their antitumor function in breast cancer., PMID:36324077

Relationship between neutralizing and opsonizing monoclonal antibodies against foot-and-mouth disease virus., PMID:36311653

Relationship between neutralizing and opsonizing monoclonal antibodies against foot-and-mouth disease virus., PMID:36311653

Direct CD32 T-cell cytotoxicity: implications for breast cancer prognosis and treatment., PMID:36241426

Direct CD32 T-cell cytotoxicity: implications for breast cancer prognosis and treatment., PMID:36241426

Innate cell markers that predict anti-HIV neutralizing antibody titers in vaccinated macaques., PMID:36167072

Innate cell markers that predict anti-HIV neutralizing antibody titers in vaccinated macaques., PMID:36167072

A cytotoxic effect of human lactoferrin fusion with Fc domain of IgG., PMID:36136256

A cytotoxic effect of human lactoferrin fusion with Fc domain of IgG., PMID:36136256

Down-regulated FcγRII expression on plasma cells is associated with the disease activity of ANCA-associated vasculitis., PMID:36111855

Down-regulated FcγRII expression on plasma cells is associated with the disease activity of ANCA-associated vasculitis., PMID:36111855

Anticalin N- or C-Terminal on a Monoclonal Antibody Affects Both Production and In Vitro Functionality., PMID:35997348

Anticalin N- or C-Terminal on a Monoclonal Antibody Affects Both Production and In Vitro Functionality., PMID:35997348

Zika Virus Replication in a Mast Cell Model is Augmented by Dengue Virus Antibody-Dependent Enhancement and Features a Selective Immune Mediator Secretory Profile., PMID:35862953

Zika Virus Replication in a Mast Cell Model is Augmented by Dengue Virus Antibody-Dependent Enhancement and Features a Selective Immune Mediator Secretory Profile., PMID:35862953

Identification of HIV-reservoir cells with reduced susceptibility to antibody-dependent immune response., PMID:35616530

Identification of HIV-reservoir cells with reduced susceptibility to antibody-dependent immune response., PMID:35616530

Spontaneous Platelet Aggregation in Blood Is Mediated by FcγRIIA Stimulation of Bruton's Tyrosine Kinase., PMID:35008508

Spontaneous Platelet Aggregation in Blood Is Mediated by FcγRIIA Stimulation of Bruton's Tyrosine Kinase., PMID:35008508

An Afucosylated Anti-CD32b Monoclonal Antibody Induced Platelet-Mediated Adverse Events in a Human Fcγ Receptor Transgenic Mouse Model and Its Potential Human Translatability., PMID:34687301

An Afucosylated Anti-CD32b Monoclonal Antibody Induced Platelet-Mediated Adverse Events in a Human Fcγ Receptor Transgenic Mouse Model and Its Potential Human Translatability., PMID:34687301

Immunoglobulin G Immune Complexes May Contribute to Neutrophil Activation in the Course of Severe Coronavirus Disease 2019., PMID:34398243

Immunoglobulin G Immune Complexes May Contribute to Neutrophil Activation in the Course of Severe Coronavirus Disease 2019., PMID:34398243

CD32+CD4+ T Cells Sharing B Cell Properties Increase With Simian Immunodeficiency Virus Replication in Lymphoid Tissues., PMID:34220857

CD32+CD4+ T Cells Sharing B Cell Properties Increase With Simian Immunodeficiency Virus Replication in Lymphoid Tissues., PMID:34220857

Systemic and mucosal mobilization of granulocyte subsets during lentiviral infection., PMID:34037988

Systemic and mucosal mobilization of granulocyte subsets during lentiviral infection., PMID:34037988

Are Fc Gamma Receptor Polymorphisms Important in HIV-1 Infection Outcomes and Latent Reservoir Size?, PMID:34017334

Are Fc Gamma Receptor Polymorphisms Important in HIV-1 Infection Outcomes and Latent Reservoir Size?, PMID:34017334

Comparison of diagnostic performances of ten different immunoassays detecting anti-CCHFV IgM and IgG antibodies from acute to subsided phases of Crimean-Congo hemorrhagic fever., PMID:33720942

Comparison of diagnostic performances of ten different immunoassays detecting anti-CCHFV IgM and IgG antibodies from acute to subsided phases of Crimean-Congo hemorrhagic fever., PMID:33720942

Platelet-activating immune complexes identified in critically ill COVID-19 patients suspected of heparin-induced thrombocytopenia., PMID:33639037

Platelet-activating immune complexes identified in critically ill COVID-19 patients suspected of heparin-induced thrombocytopenia., PMID:33639037

Genetic variation in the immune system and malaria susceptibility in infants: a nested case-control study in Nanoro, Burkina Faso., PMID:33593344

Genetic variation in the immune system and malaria susceptibility in infants: a nested case-control study in Nanoro, Burkina Faso., PMID:33593344

Cyclophosphamide alters the tumor cell secretome to potentiate the anti-myeloma activity of daratumumab through augmentation of macrophage-mediated antibody dependent cellular phagocytosis., PMID:33552684

Cyclophosphamide alters the tumor cell secretome to potentiate the anti-myeloma activity of daratumumab through augmentation of macrophage-mediated antibody dependent cellular phagocytosis., PMID:33552684

Human peripheral basophils extended phenotype shows a high expression of CD244 immuno-regulatory receptor., PMID:33493550

Human peripheral basophils extended phenotype shows a high expression of CD244 immuno-regulatory receptor., PMID:33493550

Scleroderma-specific autoantibodies embedded in immune complexes mediate endothelial damage: an early event in the pathogenesis of systemic sclerosis., PMID:33168071

Scleroderma-specific autoantibodies embedded in immune complexes mediate endothelial damage: an early event in the pathogenesis of systemic sclerosis., PMID:33168071

Basal Levels of CD18 Antigen Presenting Cells in Cow Milk Associate with Copy Number Variation of Fc Gamma Receptors., PMID:32824852

Basal Levels of CD18 Antigen Presenting Cells in Cow Milk Associate with Copy Number Variation of Fc Gamma Receptors., PMID:32824852

Quantitative immunohistochemical analysis of myeloid cell marker expression in human cortex captures microglia heterogeneity with anatomical context., PMID:32678124

Quantitative immunohistochemical analysis of myeloid cell marker expression in human cortex captures microglia heterogeneity with anatomical context., PMID:32678124

FcγRII (CD32) modulates antibody clearance in NOD SCID mice leading to impaired antibody-mediated tumor cell deletion., PMID:32554613

FcγRII (CD32) modulates antibody clearance in NOD SCID mice leading to impaired antibody-mediated tumor cell deletion., PMID:32554613

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human CD32/FcRII/Ly-17 Polyclonal Antibody [PHK22801]
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