Buy Anti-Human KISS1R Polyclonal Antibody

Catalog No.

PHJ36401

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human KISS1R (His2-Trp43).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

KiSS-1R, G-protein coupled receptor 54, KiSS-1 receptor, GPR54, KISS1R, hOT7T175, G-protein coupled receptor OT7T175, Metastin receptor, Hypogonadotropin-1, AXOR12, Kisspeptins receptor

Purification

Purified by antigen affinity column.

Accession

Q969F8

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Western Blot

Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with KISS1R antibody (PHJ36401) at 1 μg/ml.

Lane 1: Recombinant protein

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

Predict MW: 16 kDa
Observed MW: 16 kDa

Acid-Resistant BODIPY Amino Acids for Peptide-Based Fluorescence Imaging of GPR54 Receptors in Pancreatic Islets., PMID:38516305

Acid-Resistant BODIPY Amino Acids for Peptide-Based Fluorescence Imaging of GPR54 Receptors in Pancreatic Islets., PMID:36917014

Role of the kisspeptin/KISS1 receptor system in the testicular development of mice., PMID:33543882

Role of the Kisspeptin/KISS1 Receptor System in the Testicular Development of Mice., PMID:33044408

Kiss1R Identification and Biodistribution Analysis Employing a Western Ligand Blot and Ligand-Derivative Stain with a FITC-Kisspeptin Derivative., PMID:32706162

Immunoreactivity of Kisspeptin and Kisspeptin Receptor in Eutopic and Ectopic Endometrial Tissue of Women With and Without Endometriosis., PMID:32072605

[Kisspeptins in human ovarian tissue during ontogenesis.]., PMID:31800179

Kisspeptin recombinant oral vaccine: A master gene vaccine inhibiting the reproductive physiology and behavior of ram lambs., PMID:29097006

Characterisation of Kiss1r (Gpr54)-Expressing Neurones in the Arcuate Nucleus of the Female Rat Hypothalamus., PMID:27981646

The KISS1 Receptor as an In Vivo Microenvironment Imaging Biomarker of Multiple Myeloma Bone Disease., PMID:27158817

Neuronal connectivity between habenular glutamate-kisspeptin1 co-expressing neurons and the raphe 5-HT system., PMID:26250886

Kiss-1/GPR54 protein expression in breast cancer., PMID:24596387

Kisspeptin effect on endothelial monocyte activating polypeptide II (EMAP-II)-associated lymphocyte cell death and metastases in colorectal cancer patients., PMID:24395571

Kisspeptin/KISS1R signaling potentiates extravillous trophoblast adhesion to type-I collagen in a PKC- and ERK1/2-dependent manner., PMID:24273038

Development and validation of sensitive sandwich ELISAs for two investigational nonapeptide metastin receptor agonists, TAK-448 and TAK-683., PMID:22748666

Hyperalgesic activity of kisspeptin in mice., PMID:22112588

Mutagenesis and analysis of genetic mutations in the GC-rich KISS1 receptor sequence identified in humans with reproductive disorders., PMID:21912371

Organization of two independent kisspeptin systems derived from evolutionary-ancient kiss genes in the brain of zebrafish., PMID:21325050

KISS1R intracellular trafficking and degradation: effect of the Arg386Pro disease-associated mutation., PMID:21285314

Expression of functional KISS1 and KISS1R system is altered in human pituitary adenomas: evidence for apoptotic action of kisspeptin-10., PMID:21169415

Assessment of constitutive activity and internalization of GPR54 (KISS1-R)., PMID:21036227

The effects of Kisspeptin antibodies on delayed estrus in rats., PMID:20222841

Distribution of kisspeptin neurones in the adult female mouse brain., PMID:19515163

The distribution of kisspeptin and its receptor GPR54 in rat dorsal root ganglion and up-regulation of its expression after CFA injection., PMID:19111911

Hypothalamic control of anterior pituitary function: a history., PMID:18601683

KiSS-1 and GPR54 genes are co-expressed in rat gonadotrophs and differentially regulated in vivo by oestradiol and gonadotrophin-releasing hormone., PMID:18208554

Requirement of KISS1 secretion for multiple organ metastasis suppression and maintenance of tumor dormancy., PMID:17312308

Involvement of anteroventral periventricular metastin/kisspeptin neurons in estrogen positive feedback action on luteinizing hormone release in female rats., PMID:17213691

KiSS-1 expression in human breast cancer., PMID:16320113

AXOR12, a novel human G protein-coupled receptor, activated by the peptide KiSS-1., PMID:11387329

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Anti-KISS1R Polyclonal Antibody