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Anti-Human POU3F3 Polyclonal Antibody

Catalog #:   PHD38301 Specific References (5) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P20264
Overview

Catalog No.

PHD38301

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human POU3F3 (Thr316-Gln403).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

POU3F3, Oct-8, OTF-8, BRN1, POU domain, class 3, transcription factor 3, Brain-1, Octamer-binding transcription factor 8, OTF8, Brn-1, Brain-specific homeobox/POU domain protein 1, Octamer-binding protein 8

Purification

Purified by antigen affinity column.

Accession

P20264

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with POU3F3 antibody (PHD38301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 22 kDa
    Observed MW: 22 kDa
References

Knockdown of linc-POU3F3 suppresses the proliferation, apoptosis, and migration resistance of colorectal cancer., PMID:26510906

Characterization of enhancers active in the mouse embryonic cerebral cortex suggests Sox/Pou cis-regulatory logics and heterogeneity of cortical progenitors., PMID:23720416

Expression of Pou3f3/Brn-1 and its genomic methylation in developing auditory epithelium., PMID:19743445

Distinctive gene expression signatures in rheumatoid arthritis synovial tissue fibroblast cells: correlates with disease activity., PMID:17568789

Hox/Pbx and Brn binding sites mediate Pax3 expression in vitro and in vivo., PMID:15465489

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human POU3F3 Polyclonal Antibody [PHD38301]
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