Catalog No.
PHA74801
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human BUB1 (Met1-His151).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
BUB1L, hBUB1, BUB1A, Mitotic checkpoint serine/threonine-protein kinase BUB1, BUB1
Purification
Purified by antigen affinity column.
Accession
O43683
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Phosphorylation of Bub1 by Mph1 promotes Bub1 signaling at the kinetochore to ensure accurate chromosome segregation., PMID:38097187
[New concepts in multiple myeloma by returning to cytogenetics]., PMID:37271533
SV40 T-Antigen Amino Acid Changes that Disrupt Cul-7 or Bub-1 Binding Do Not Globally Distort the T-Common Region., PMID:27376672
OTSSP167 Abrogates Mitotic Checkpoint through Inhibiting Multiple Mitotic Kinases., PMID:27082996
The Bub1-Plk1 kinase complex promotes spindle checkpoint signalling through Cdc20 phosphorylation., PMID:26912231
Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation., PMID:26399325
Predictive and prognostic values of BubR1 and synuclein-gamma expression in breast cancer., PMID:26191236
Distinct domains in Bub1 localize RZZ and BubR1 to kinetochores to regulate the checkpoint., PMID:26031201
Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes., PMID:26028219
Deregulation of HMGA1 expression induces chromosome instability through regulation of spindle assembly checkpoint genes., PMID:26009897
Overexpression of the ∆Np73 isoform is associated with centrosome amplification in brain tumor cell lines., PMID:25910708
The kinase activity of the Ser/Thr kinase BUB1 promotes TGF-β signaling., PMID:25564677
Evaluation of 2',4'-dihydroxy-3,4,5-trimethoxychalcone as antimitotic agent that induces mitotic catastrophe in MCF-7 breast cancer cells., PMID:24968064
BuGZ is required for Bub3 stability, Bub1 kinetochore function, and chromosome alignment., PMID:24462187
Mechanism of mitotic arrest induced by dolastatin 15 involves loss of tension across kinetochore pairs., PMID:23744533
Predictive and prognostic values of Tau and BubR1 protein in prostate cancer and their relationship to the Gleason score., PMID:23475578
Elevated BUBR1 expression is associated with poor survival in early breast cancer patients: 15-year follow-up analysis., PMID:23392733
Aging and neoteny in the B lineage., PMID:22936664
Spindle assembly checkpoint signalling is uncoupled from chromosomal position in mouse oocytes., PMID:22513372
Structure of human Mad1 C-terminal domain reveals its involvement in kinetochore targeting., PMID:22493223
BRCA2 fine-tunes the spindle assembly checkpoint through reinforcement of BubR1 acetylation., PMID:22340495
Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction., PMID:22331848
DNA damage response signaling triggers nuclear localization of the chicken anemia virus protein Apoptin., PMID:21937663
Effects of artesunate on cytokinesis and G₂/M cell cycle progression of tumour cells and budding yeast., PMID:21156967
Dicer is a key player in oocyte maturation., PMID:20827505
MCAK is present at centromeres, midspindle and chiasmata and involved in silencing of the spindle assembly checkpoint in mammalian oocytes., PMID:20406800
Antibody-based proteomics for esophageal cancer: Identification of proteins in the nuclear factor-kappaB pathway and mitotic checkpoint., PMID:19558549
Inhibitory effect of wild-type p53 gene on excessive replication of centrosomes in leukemia cell line K562., PMID:19550118
BubR1 acetylation at prometaphase is required for modulating APC/C activity and timing of mitosis., PMID:19407811
hBub1 negatively regulates p53 mediated early cell death upon mitotic checkpoint activation., PMID:19252416
Metaphase I arrest in LT/Sv mouse oocytes involves the spindle assembly checkpoint., PMID:18753610
Mitotic arrest-associated apoptosis induced by sodium arsenite in A375 melanoma cells is BUBR1-dependent., PMID:18501396
HBV X protein targets hBubR1, which induces dysregulation of the mitotic checkpoint., PMID:18193091
Tension-sensitive Plk1 phosphorylation on BubR1 regulates the stability of kinetochore microtubule interactions., PMID:17785528
p53 activation in response to mitotic spindle damage requires signaling via BubR1-mediated phosphorylation., PMID:17671183
Preparation of monoclonal antibodies against the spindle checkpoint kinase Bub1., PMID:17600495
Subcellular localization of the spindle proteins Aurora A, Mad2, and BUBR1 assessed by immunohistochemistry., PMID:17242465
Combretastatin CA-4 and combretastatin derivative induce mitotic catastrophe dependent on spindle checkpoint and caspase-3 activation in non-small cell lung cancer cells., PMID:17143747
Bub1 prevents chromosome misalignment and precocious anaphase during mouse oocyte meiosis., PMID:16969117
Loading of the 3F3/2 antigen onto kinetochores is dependent on the ordered assembly of the spindle checkpoint proteins., PMID:16885416
Human Scc4 is required for cohesin binding to chromatin, sister-chromatid cohesion, and mitotic progression., PMID:16682347
BubR1 is involved in regulation of DNA damage responses., PMID:16449973
Monoallelic BUB1B mutations and defective mitotic-spindle checkpoint in seven families with premature chromatid separation (PCS) syndrome., PMID:16411201
Microtubule capture by CENP-E silences BubR1-dependent mitotic checkpoint signaling., PMID:16144904
Ablation of the spindle assembly checkpoint by a compound targeting Mps1., PMID:16113653
Frequent mutations of human Mad2, but not Bub1, in gastric cancers cause defective mitotic spindle checkpoint., PMID:16112690
Mitotic spindle checkpoint inactivation by trichostatin a defines a mechanism for increasing cancer cell killing by microtubule-disrupting agents., PMID:15753652
Genomic and cytological analysis of the Y chromosome of Drosophila melanogaster: telomere-derived sequences at internal regions., PMID:15616866
The mitotic arrest in response to hypoxia and of polar bodies during early embryogenesis requires Drosophila Mps1., PMID:15556864
BAF53/Arp4 homolog Alp5 in fission yeast is required for histone H4 acetylation, kinetochore-spindle attachment, and gene silencing at centromere., PMID:15483052
For research use only. Not for human or drug use.
