Catalog No.
MVV11001
Species reactivity
African swine fever virus (ASFV)
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Recombinant ASFV NS4A protein.
Target
nonstructural protein NS4A, nonstructural protein NS4A
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
NP_777503.1
Applications
ELISA, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
1A017
Differential requirements of NS4A for internal NS3 cleavage and polyprotein processing of hepatitis C virus. PMID: 17522200
Dengue Virus Non-Structural Protein 5. PMID: 28441781
Hepatitis C virus NS4A inhibits cap-dependent and the viral IRES-mediated translation through interacting with eukaryotic elongation factor 1A. PMID: 16927014
A central region in the hepatitis C virus NS4A protein allows formation of an active NS3-NS4A serine proteinase complex in vivo and in vitro. PMID: 7769699
Modulation of Kinase Activities In Vitro by Hepatitis C Virus Protease NS3/NS4A Mediated-Cleavage of Key Immune Modulator Kinases. PMID: 36766748
Baseline anti-NS4a antibodies in combination with on-treatment quantitative HCV-RNA reliably identifies nonresponders to pegylated interferon-ribavirin combination therapy after 4 weeks of treatment. PMID: 21389795
A Putative Lipid-Associating Motif in the West Nile Virus NS4A Protein Is Required for Efficient Virus Replication. PMID: 34055786
Grazoprevir/elbasvir fixed-dose combination for hepatitis C. PMID: 27540596
Nonstructural protein precursor NS4A/B from hepatitis C virus alters function and ultrastructure of host secretory apparatus. PMID: 12829824
Use of the fused NS4A peptide-NS3 protease domain to study the importance of the helicase domain for protease inhibitor binding to hepatitis C virus NS3-NS4A. PMID: 19119853
Roles of Non-Structural Protein 4A in Flavivirus Infection. PMID: 34696510
The acidic domain of hepatitis C virus NS4A contributes to RNA replication and virus particle assembly. PMID: 21047963
Conserved amino acids within the N-terminus of the West Nile virus NS4A protein contribute to virus replication, protein stability and membrane proliferation. PMID: 25771497
Zika Virus Proteins NS2A and NS4A Are Major Antagonists that Reduce IFN-β Promoter Activity Induced by the MDA5/RIG-I Signaling Pathway. PMID: 31581385
Expression of NS3/NS4A Proteins of Hepatitis C Virus in Huh7 Cells Following Engineering Its Eukaryotic Expression Vector. PMID: 26862385
Regulated cleavages at the West Nile virus NS4A-2K-NS4B junctions play a major role in rearranging cytoplasmic membranes and Golgi trafficking of the NS4A protein. PMID: 16611922
Codon usage variation of Zika virus: The potential roles of NS2B and NS4A in its global pandemic. PMID: 29428601
Inhibition of hepatitis C virus (HCV) core protein- induced cell growth by non-structural protein 4A (NS4A) is mediated by mitochondrial dysregulation. PMID: 18318665
Neuroimmune Evasion of Zika Virus to Facilitate Viral Pathogenesis. PMID: 34765564
Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. PMID: 24284321
Expression and purification of an active, full-length hepatitis C viral NS4A. PMID: 11049744
Interferon antagonist function of Japanese encephalitis virus NS4A and its interaction with DEAD-box RNA helicase DDX42. PMID: 18588927
Hepatitis C virus RNA replication and virus particle assembly require specific dimerization of the NS4A protein transmembrane domain. PMID: 24173222
Restoration of replication-defective dengue type 1 virus bearing mutations in the N-terminal cytoplasmic portion of NS4A by additional mutations in NS4B. PMID: 20882304
Zika Virus NS4A and NS4B Proteins Deregulate Akt-mTOR Signaling in Human Fetal Neural Stem Cells to Inhibit Neurogenesis and Induce Autophagy. PMID: 27524440
A novel interaction between dengue virus nonstructural protein 1 and the NS4A-2K-4B precursor is required for viral RNA replication but not for formation of the membranous replication organelle. PMID: 31071189
Both NS3 and NS4A are required for proteolytic processing of hepatitis C virus nonstructural proteins. PMID: 8189513
Enhancement of hepatitis C virus NS3 proteinase activity by association with NS4A-specific synthetic peptides: identification of sequence and critical residues of NS4A for the cofactor activity. PMID: 8918919
A novel recombinant single-chain hepatitis C virus NS3-NS4A protein with improved helicase activity. PMID: 10386883
Identification of the sequence on NS4A required for enhanced cleavage of the NS5A/5B site by hepatitis C virus NS3 protease. PMID: 8523516
Faldaprevir for the treatment of hepatitis C. PMID: 26097902
Discovery of Dengue Virus Inhibitors. PMID: 30514185
Nonstructural proteins NS2-3 and NS4A of classical swine fever virus: essential features for infectious particle formation. PMID: 17482232
Cross-genotypic interaction between hepatitis C virus NS3 protease domains and NS4A cofactors. PMID: 10735621
Rational design and functional expression of a constitutively active single-chain NS4A-NS3 proteinase. PMID: 9889156
Interferon-free strategies without a nucleoside/nucleotide analogue. PMID: 24782258
Modulation of hepatitis C virus NS3 protease and helicase activities through the interaction with NS4A. PMID: 10220351
Characterization of clinical predictors of naturally occurring NS3/NS4A protease polymorphism in genotype 1 hepatitis C virus mono and HIV co-infected patients. PMID: 28700085
Complex formation between the NS3 serine-type proteinase of the hepatitis C virus and NS4A and its importance for polyprotein maturation. PMID: 7494258
Classical Swine Fever Virus Infection and Its NS4A Protein Expression Induce IL-8 Production through MAVS Signaling Pathway in Swine Umbilical Vein Endothelial Cells. PMID: 29375538
In vitro studies on the activation of the hepatitis C virus NS3 proteinase by the NS4A cofactor. PMID: 8661414
Dengue Virus NS Proteins Inhibit RIG-I/MAVS Signaling by Blocking TBK1/IRF3 Phosphorylation: Dengue Virus Serotype 1 NS4A Is a Unique Interferon-Regulating Virulence Determinant. PMID: 25968648
Identification of three H-2K(d) restricted CTL epitopes of NS4A and NS4B protein from Yellow fever 17D vaccine. PMID: 23123122
Selection of functional variants of the NS3-NS4A protease of hepatitis C virus by using chimeric sindbis viruses. PMID: 9847361
Molecular characterization and fluorescence analysis of HCV non-structural proteins NS3, NS3-4A and NS4A of genotype 3a. PMID: 26435152
HCV NS3-4A Serine Protease. PMID: 21250386
Dengue virus NS2 and NS4: Minor proteins, mammoth roles. PMID: 29674002
The acidic sequence of the NS4A cofactor regulates ATP hydrolysis by the HCV NS3 helicase. PMID: 20978807
An amino acid substitution (V3I) in the Japanese encephalitis virus NS4A protein increases its virulence in mice, but not its growth rate in vitro. PMID: 21411676
Hepatitis C virus NS4A and NS4B proteins suppress translation in vivo. PMID: 11782927
Sterile 20-like kinase 3 promotes tick-borne encephalitis virus assembly by interacting with NS2A and prM and enhancing the NS2A-NS4A association. PMID: 36840407
Hepatitis C virus NS3/NS4A DNA vaccine induces multiepitope T cell responses in rhesus macaques mimicking human immune responses [corrected]. PMID: 21952169
Mechanistic and kinetic characterization of hepatitis C virus NS3 protein interactions with NS4A and protease inhibitors. PMID: 21194118
Phylogenetic and 2D/3D Analysis of HCV 1a NS4A Gene/Protein in Pakistani Isolates. PMID: 26288631
Innate immunity evasion by Dengue virus. PMID: 22590678
Solution structure and dynamics of the single-chain hepatitis C virus NS3 protease NS4A cofactor complex. PMID: 11162117
Complex of NS3 protease and NS4A peptide of BK strain hepatitis C virus: a 2.2 A resolution structure in a hexagonal crystal form. PMID: 9568891
Molecular mechanism by which a potent hepatitis C virus NS3-NS4A protease inhibitor overcomes emergence of resistance. PMID: 23271737
Recovery of a chemically synthesized Japanese encephalitis virus reveals two critical adaptive mutations in NS2B and NS4A. PMID: 24362961
Expression of a hepatitis C virus NS3 protease-NS4A fusion protein in Escherichia coli. PMID: 9571178
Drug design for flavivirus proteases: what are we missing? PMID: 24001226
A comparative QM/MM study of the reaction mechanism of the Hepatitis C virus NS3/NS4A protease with the three main natural substrates NS5A/5B, NS4B/5A and NS4A/4B. PMID: 20520921
Positive identification of NS4A, the last of the hypothetical nonstructural proteins of flaviviruses. PMID: 2541547
Virulence determinants of West Nile virus: how can these be used for vaccine design? PMID: 28919920
Research progress on the direct antiviral drugs for hepatitis C virus. PMID: 28100890
For research use only. Not for human or drug use.
