Catalog No.
YHF54701
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Ala2-Arg46
Predicted molecular weight
33.05 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P62877
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
RING finger protein 75, RNF75, E3 ubiquitin-protein transferase RBX1, N-terminally processed, RBX1, E3 ubiquitin-protein ligase RBX1, Rbx1, ROC1, E3 ubiquitin-protein transferase RBX1, Protein ZYP, RING-box protein 1, Regulator of cullins 1
SQSTM1/p62 activates NFE2L2/NRF2 via ULK1-mediated autophagic KEAP1 degradation and protects mouse liver from lipotoxicity. PMID: 31913745
Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex. PMID: 11961546
AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions. PMID: 32391793
Regulation of Nrf2-an update. PMID: 23434765
The E3 ubiquitin-protein ligase Rbx1 regulates cardiac wall morphogenesis in zebrafish. PMID: 34363825
Identification of a primary target of thalidomide teratogenicity. PMID: 20223979
Structure of the DDB1-CRBN E3 ubiquitin ligase in complex with thalidomide. PMID: 25043012
Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation. PMID: 32804079
Exploring Shared Pathogenesis of Alzheimer's Disease and Type 2 Diabetes Mellitus via Co-expression Networks Analysis. PMID: 32781959
Mitochondrial Stasis Reveals p62-Mediated Ubiquitination in Parkin-Independent Mitophagy and Mitigates Nonalcoholic Fatty Liver Disease. PMID: 30017357
Suppression of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2 polyubiquitination and proteasomal degradation. PMID: 30898011
Prognostic impact of RING box protein-1 (RBX1) expression in gastric cancer. PMID: 24292229
Development of a BCL-xL and BCL-2 dual degrader with improved anti-leukemic activity. PMID: 34824248
Introduction. PMID: 31898218
Overexpression of RING box protein-1 (RBX1) associated with poor prognosis of non-muscle-invasive bladder transitional cell carcinoma. PMID: 23609182
RBX1 (RING box protein 1) E3 ubiquitin ligase is required for genomic integrity by modulating DNA replication licensing proteins. PMID: 21115485
Endoplasmic reticulum stress induces a caspase-dependent N-terminal cleavage of RBX1 protein in B cells. PMID: 22822056
CUL5-ARIH2 E3-E3 ubiquitin ligase structure reveals cullin-specific NEDD8 activation. PMID: 34518685
RING box protein-1 gene involved in flagellar disassembly of Dunaliella salina. PMID: 27655397
RBX1/ROC1-SCF E3 ubiquitin ligase is required for mouse embryogenesis and cancer cell survival. PMID: 19660140
Ring box protein-1 is associated with a poor prognosis and tumor progression in esophageal cancer. PMID: 32782608
ROC1 promotes the malignant progression of bladder cancer by regulating p-IκBα/NF-κB signaling. PMID: 33962660
Role of the Arabidopsis RING-H2 protein RBX1 in RUB modification and SCF function. PMID: 12215511
The characteristics and roles of β-TrCP1/2 in carcinogenesis. PMID: 33021036
RBX1 expression is an unfavorable prognostic factor in patients with non-small cell lung cancer. PMID: 27566015
Non-coding RNAs and related molecules associated with form-deprivation myopia in mice. PMID: 34841657
Cullin 3 and Its Role in Tumorigenesis. PMID: 31898229
An autoregulatory loop between Nrf2 and Cul3-Rbx1 controls their cellular abundance. PMID: 20452971
Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase. PMID: 11384984
Structure of the human Cereblon-DDB1-lenalidomide complex reveals basis for responsiveness to thalidomide analogs. PMID: 25108355
Structural basis of lenalidomide-induced CK1α degradation by the CRL4(CRBN) ubiquitin ligase. PMID: 26909574
The functions and effects of CUL3-E3 ligases mediated non-degradative ubiquitination. PMID: 35580799
The glomuvenous malformation protein Glomulin binds Rbx1 and regulates cullin RING ligase-mediated turnover of Fbw7. PMID: 22405651
E3 ubiquitin ligase RBX1 drives the metastasis of triple negative breast cancer through a FBXO45-TWIST1-dependent degradation mechanism. PMID: 35802537
Identification and functional characterization of an Rbx1 in an invertebrate Haliotis diversicolor supertexta. PMID: 20801156
F-box proteins: the key to protein degradation. PMID: 16463014
miR-194 targets RBX1 gene to modulate proliferation and migration of gastric cancer cells. PMID: 25412959
The Ube2m-Rbx1 neddylation-Cullin-RING-Ligase proteins are essential for the maintenance of Regulatory T cell fitness. PMID: 35641500
RBX1-mediated ubiquitination of SESN2 promotes cell death upon prolonged mitochondrial damage in SH-SY5Y neuroblastoma cells. PMID: 29294217
Arabidopsis jasmonate signaling pathway. PMID: 20159850
The Roles of Cullin-2 E3 Ubiquitin Ligase Complex in Cancer. PMID: 31898228
Zeroing in on cereblon. PMID: 25777766
Both Rbx1 and Rbx2 exhibit a functional role in the HIV-1 Vif-Cullin5 E3 ligase complex in vitro. PMID: 25912140
RBX1 prompts degradation of EXO1 to limit the homologous recombination pathway of DNA double-strand break repair in G1 phase. PMID: 31562368
F-box proteins and protein degradation: an emerging theme in cellular regulation. PMID: 11117256
Reduced RBX1 expression induces chromosome instability and promotes cellular transformation in high-grade serous ovarian cancer precursor cells. PMID: 33290867
Crystal Structure of the Cul2-Rbx1-EloBC-VHL Ubiquitin Ligase Complex. PMID: 28591624
Genetically engineered mouse models for functional studies of SKP1-CUL1-F-box-protein (SCF) E3 ubiquitin ligases. PMID: 23528706
The Rbx1 subunit of SCF and VHL E3 ubiquitin ligase activates Rub1 modification of cullins Cdc53 and Cul2. PMID: 10579999
Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. PMID: 12149480
Structure of a glomulin-RBX1-CUL1 complex: inhibition of a RING E3 ligase through masking of its E2-binding surface. PMID: 22748924
Caenorhabditis elegans RBX1 is essential for meiosis, mitotic chromosomal condensation and segregation, and cytokinesis. PMID: 14622138
Role of SKP1-CUL1-F-box-protein (SCF) E3 ubiquitin ligases in skin cancer. PMID: 23522382
SCFs in the new millennium. PMID: 23624913
Structure of CRL7FBXW8 reveals coupling with CUL1-RBX1/ROC1 for multi-cullin-RING E3-catalyzed ubiquitin ligation. PMID: 35982156
RBX1/ROC1 disruption results in early embryonic lethality due to proliferation failure, partially rescued by simultaneous loss of p27. PMID: 19325126
Multiple roles of Rbx1 in the VBC-Cul2 ubiquitin ligase complex. PMID: 15966899
ELF1-mediated LUCAT1 promotes choroidal melanoma by modulating RBX1 expression. PMID: 31968402
ASB2 is an Elongin BC-interacting protein that can assemble with Cullin 5 and Rbx1 to reconstitute an E3 ubiquitin ligase complex. PMID: 15590664
Protective autophagy induced by RBX1/ROC1 knockdown or CRL inactivation via modulating the DEPTOR-MTOR axis. PMID: 22965024
MiR-135b-5p inhibits the progression of malignant melanoma cells by targeting RBX1. PMID: 32096167
Modeling the CRL4A ligase complex to predict target protein ubiquitination induced by cereblon-recruiting PROTACs. PMID: 35101445
Cullin 2-RBX1 E3 ligase and USP2 regulate antithrombin ubiquitination and stability. PMID: 34324733
Arsenite Targets the RING Finger Domain of Rbx1 E3 Ubiquitin Ligase to Inhibit Proteasome-Mediated Degradation of Nrf2. PMID: 29658272
High-Affinity Peptidomimetic Inhibitors of the DCN1-UBC12 Protein-Protein Interaction. PMID: 29438612
The role of RING box protein 1 in mouse oocyte meiotic maturation. PMID: 23874827
Cullin-RING E3 Ubiquitin Ligase 7 in Growth Control and Cancer. PMID: 31898234
Cancer-testis antigen MAGE-C2 binds Rbx1 and inhibits ubiquitin ligase-mediated turnover of cyclin E. PMID: 26540345
The Cullin-3-Rbx1-KCTD10 complex controls endothelial barrier function via K63 ubiquitination of RhoB. PMID: 29358211
Destruction of DDIT3/CHOP protein by wild-type SPOP but not prostate cancer-associated mutants. PMID: 24990631
A homolog of the E3 ubiquitin ligase Rbx1 is induced during hyperosmotic stress of salmon. PMID: 12010746
Knockout Mouse Models Provide Insight into the Biological Functions of CRL1 Components. PMID: 31898227
Monitoring Keap1-Nrf2 interactions in single live cells. PMID: 24681086
Identification of the self-incompatibility locus F-box protein-containing complex in Petunia inflata. PMID: 24381071
Role of Nrf2 in bisphenol effects: a review study. PMID: 35680748
The Arabidopsis ALF4 protein is a regulator of SCF E3 ligases. PMID: 29233834
Characterization of SCF-Complex during Bovine Preimplantation Development. PMID: 26824694
A20 and RBX1 Regulate Brentuximab Vedotin Sensitivity in Hodgkin Lymphoma Models. PMID: 32299816
Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase. PMID: 10213691
Post-translational regulation of the cellular levels of DAPK. PMID: 24185832
Gossypol inhibits cullin neddylation by targeting SAG-CUL5 and RBX1-CUL1 complexes. PMID: 32145688
Genomic organization and expression of the ubiquitin-proteasome complex-associated protein Rbx1/ROC1/Hrt1. PMID: 10643962
Adenovirus ubiquitin-protein ligase stimulates viral late mRNA nuclear export. PMID: 17079297
The F-box protein FBXO25 promotes the proteasome-dependent degradation of ELK-1 protein. PMID: 23940030
Rbx1 flexible linker facilitates cullin-RING ligase function before neddylation and after deneddylation. PMID: 20682250
CUL4A-DDB1-Rbx1 E3 ligase controls the quality of the PTS2 receptor Pex7p. PMID: 24989250
A hitchhiker's guide to the cullin ubiquitin ligases: SCF and its kin. PMID: 15571813
ROC1/RBX1 E3 ubiquitin ligase silencing suppresses tumor cell growth via sequential induction of G2-M arrest, apoptosis, and senescence. PMID: 19509229
WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase. PMID: 18381890
The proteome reveals the involvement of serine/threonine kinase in the recognition of self- incompatibility in almond. PMID: 35123051
ΔNp73 enhances HIF-1α protein stability through repression of the ECV complex. PMID: 29628507
Involvement of F-BOX proteins in progression and development of human malignancies. PMID: 26410033
Comparative Proteomics Reveals Strain-Specific β-TrCP Degradation via Rotavirus NSP1 Hijacking a Host Cullin-3-Rbx1 Complex. PMID: 27706223
SPOP targets oncogenic protein ZBTB3 for destruction to suppress endometrial cancer. PMID: 31911863
Small RING Finger Proteins RBX1 and RBX2 of SCF E3 Ubiquitin Ligases: The Role in Cancer and as Cancer Targets. PMID: 21103004
Emergence of unique SARS-CoV-2 ORF10 variants and their impact on protein structure and function. PMID: 34863825
Structural Insights into KCTD Protein Assembly and Cullin3 Recognition. PMID: 26334369
Nrf2 signaling and cell survival. PMID: 19538984
Inhibitor of Nrf2 (INrf2 or Keap1) protein degrades Bcl-xL via phosphoglycerate mutase 5 and controls cellular apoptosis. PMID: 22072718
The emerging family of CULLIN3-RING ubiquitin ligases (CRL3s): cellular functions and disease implications. PMID: 23912815
[TGF-beta signaling]. PMID: 16922387
Nedd8 on cullin: building an expressway to protein destruction. PMID: 15021886
Prothymosin-alpha mediates nuclear import of the INrf2/Cul3 Rbx1 complex to degrade nuclear Nrf2. PMID: 19279002
Cullin-RING Ligase 5: Functional characterization and its role in human cancers. PMID: 32334051
Fbxw5 suppresses nuclear c-Myb activity via DDB1-Cul4-Rbx1 ligase-mediated sumoylation. PMID: 22910413
For research use only. Not for human or drug use.
