Catalog No.
YHK90801
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Met117-Gly325
Predicted molecular weight
26.90 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q5XXA6
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Anoctamin-1, Tumor-amplified and overexpressed sequence 2, Transmembrane protein 16A, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, TMEM16A, ANO1, TAOS2, ORAOV2, DOG1
Cepharanthine, a novel selective ANO1 inhibitor with potential for lung adenocarcinoma therapy. PMID: 34450215
Pendred syndrome. PMID: 28648509
Structure and function of TMEM16 proteins (anoctamins). PMID: 24692353
Role of ANO1 in tumors and tumor immunity. PMID: 35471604
ANO1 protein as a potential biomarker for esophageal cancer prognosis and precancerous lesion development prediction. PMID: 27016410
Suppression of CaMKIIβ Inhibits ANO1-Mediated Glioblastoma Progression. PMID: 32357567
Small molecule-facilitated degradation of ANO1 protein: a new targeting approach for anticancer therapeutics. PMID: 24599954
Opposing roles of E3 ligases TRIM23 and TRIM21 in regulation of ion channel ANO1 protein levels. PMID: 33957127
ANO1 relieves pressure overload-induced myocardial fibrosis in mice by inhibiting TGF-β/Smad3 signaling pathway. PMID: 32894555
Surface expression of the Anoctamin-1 (ANO1) channel is suppressed by protein-protein interactions with β-COP. PMID: 27207835
Involvement of TMEM16A/ANO1 upregulation in the oncogenesis of colorectal cancer. PMID: 35231545
TMEM16A (ANO1) as a therapeutic target in cystic fibrosis. PMID: 35364521
Cell-specific mechanisms of TMEM16A Ca2+-activated chloride channel in cancer. PMID: 28893247
Lysosomal inhibition sensitizes TMEM16A-expressing cancer cells to chemotherapy. PMID: 35286200
TMEM16A/ANO1: Current Strategies and Novel Drug Approaches for Cystic Fibrosis. PMID: 34831090
ANO1 regulates cardiac fibrosis via ATI-mediated MAPK pathway. PMID: 33075549
Molecular mechanisms of activation and regulation of ANO1-Encoded Ca2+-Activated Cl- channels. PMID: 34488544
[Effect of enhanced green fluorescent protein fusion on Ano1 physiological feature]. PMID: 26701639
Anoctamin 1/TMEM16A in pruritoceptors is essential for Mas-related G protein receptor-dependent itch. PMID: 35135993
Activation of Ca(2+) -activated Cl(-) channel ANO1 by localized Ca(2+) signals. PMID: 25398532
Chondroblastoma: An Update. PMID: 28557595
Ano1 as a regulator of proliferation. PMID: 21940901
Prognostic significance of ANO1 expression in cancers. PMID: 33530281
Anticancer effect of verteporfin on non-small cell lung cancer via downregulation of ANO1. PMID: 35785700
The Mechanistic Role of the Calcium-Activated Chloride Channel ANO1 in Tumor Growth and Signaling. PMID: 28293832
Transcriptional activation of ANO1 promotes gastric cancer progression. PMID: 30871776
Detection of ANO1 mRNA in PBMCs is a promising method for GISTs diagnosis. PMID: 31266974
The Ca(2+) -activated Cl(-) channel, ANO1 (TMEM16A), is a double-edged sword in cell proliferation and tumorigenesis. PMID: 24639373
Investigating the regulatory function of the ANO1-AS2 on the ANO1 gene in infertile men with asthenozoospermia and terato-asthenozoospermia. PMID: 32916161
ANO1 (TMEM16A) in pancreatic ductal adenocarcinoma (PDAC). PMID: 25163766
[Expression of ANO1 during cardiac fibroblasts differentiation and its electrophysiological characteristics as calcium-activated chloride channel protein]. PMID: 33167099
Direct repression of anoctamin 1 (ANO1) gene transcription by Gli proteins. PMID: 30802137
TRPV3-ANO1 interaction positively regulates wound healing in keratinocytes. PMID: 36690845
HCO3- Transport through Anoctamin/Transmembrane Protein ANO1/TMEM16A in Pancreatic Acinar Cells Regulates Luminal pH. PMID: 27510033
ANO1 in intramuscular interstitial cells of Cajal plays a key role in the generation of slow waves and tone in the internal anal sphincter. PMID: 28054347
Involvement of TRPV1-ANO1 Interactions in Pain-Enhancing Mechanisms. PMID: 30306512
Discovery of ANO1 Inhibitors based on Machine learning and molecule docking simulation approaches. PMID: 36842513
TMEM16A Protein: Calcium-Binding Site and its Activation Mechanism. PMID: 34749600
ANO1/TMEM16A regulates process maturation in radial glial cells in the developing brain. PMID: 31147466
Overexpression of ANO1/TMEM16A, an arterial Ca2+-activated Cl- channel, contributes to spontaneous hypertension. PMID: 25739000
Diethylstilbestrol, a Novel ANO1 Inhibitor, Exerts an Anticancer Effect on Non-Small Cell Lung Cancer via Inhibition of ANO1. PMID: 34281152
Ano1 mediates pressure-sensitive contraction frequency changes in mouse lymphatic collecting vessels. PMID: 30862712
ANO1 regulates the maintenance of stemness in glioblastoma stem cells by stabilizing EGFRvIII. PMID: 33452454
Novel ANO1 Inhibitor from Mallotus apelta Extract Exerts Anticancer Activity through Downregulation of ANO1. PMID: 32899792
Gating and anion selectivity are reciprocally regulated in TMEM16A (ANO1). PMID: 35687042
TRPV4 heats up ANO1-dependent exocrine gland fluid secretion. PMID: 29187363
Role of anoctamins in cancer and apoptosis. PMID: 24493744
Ca2+-activated chloride channel ANO1: A new regulator of osteoclast function. PMID: 35908317
LRRCA8A and ANO1 contribute to serum-induced VRAC in a Ca2+-dependent manners. PMID: 32386905
Oleic acid blocks the calcium-activated chloride channel TMEM16A/ANO1. PMID: 35202806
Inhibition of ANO1 by luteolin and its cytotoxicity in human prostate cancer PC-3 cells. PMID: 28362855
High level of ANO1 promotes pancreatic cancer growth in concert with oncogenic KRAS. PMID: 36715788
Identification of anoctamin 1 (ANO1) as a key driver of esophageal epithelial proliferation in eosinophilic esophagitis. PMID: 31647967
The Ca2+-activated Cl- channel Ano1 controls microvilli length and membrane surface area in the oocyte. PMID: 27173493
TMEM16A protein: a new identity for Ca(2+)-dependent Cl⁻ channels. PMID: 21186280
Conditional genetic deletion of Ano1 in interstitial cells of Cajal impairs Ca2+ transients and slow waves in adult mouse small intestine. PMID: 27979828
ANO1/TMEM16A interacts with EGFR and correlates with sensitivity to EGFR-targeting therapy in head and neck cancer. PMID: 25823819
Inhibition of ANO1/TMEM16A induces apoptosis in human prostate carcinoma cells by activating TNF-α signaling. PMID: 29899325
ANO1 inhibits cardiac fibrosis after myocardial infraction via TGF-β/smad3 pathway. PMID: 28539652
Emerging Perspectives on Pain Management by Modulation of TRP Channels and ANO1. PMID: 31336748
Anti-glioma effects of 2-aminothiophene-3-carboxamide derivatives, ANO1 channel blockers. PMID: 32906067
Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2. PMID: 27219012
Dynamic modulation of ANO1/TMEM16A HCO3(-) permeability by Ca2+/calmodulin. PMID: 23248295
Translational potential of targeting Anoctamin-1-Encoded Calcium-Activated chloride channels in hypertension. PMID: 36279919
Wasted TMEM16A channels are rescued by phosphatidylinositol 4,5-bisphosphate. PMID: 31683182
Enhanced expression of ANO1 in head and neck squamous cell carcinoma causes cell migration and correlates with poor prognosis. PMID: 22912841
A paradoxical increase of force development in saphenous and tail arteries from heterozygous ANO1 knockout mice. PMID: 33245843
EAVK segment "c" sequence confers Ca2+-dependent changes to the kinetics of full-length human Ano1. PMID: 28336549
Inhibition of Ca2+ -activated chloride channel ANO1 suppresses ovarian cancer through inactivating PI3K/Akt signaling. PMID: 30243029
Down-regulation of miR-9 promotes epithelial mesenchymal transition via regulating anoctamin-1 (ANO1) in CRC cells. PMID: 30803553
Use of anoctamin 1 (ANO1) to evaluate interstitial cells of Cajal in Hirschsprung's disease. PMID: 26510736
Synthesis and biological evaluation of novel Ani9 derivatives as potent and selective ANO1 inhibitors. PMID: 30347323
TMEM16A/ANO1 Inhibits Apoptosis Via Downregulation of Bim Expression. PMID: 28899969
Under pressure: Ano1 mediates pressure sensing in the lymphatic system. PMID: 30886053
[Effects of membrane protein ANO1 stable overexpression on laryngocarcinoma Hep-2 cells]. PMID: 24581123
Time for TMEM? PMID: 23204100
Ano1 is a better marker than c-Kit for transcript analysis of single interstitial cells of Cajal in culture. PMID: 25338768
Cinobufagin Exerts Anticancer Activity in Oral Squamous Cell Carcinoma Cells through Downregulation of ANO1. PMID: 34769467
Activation of the Ano1 (TMEM16A) chloride channel by calcium is not mediated by calmodulin. PMID: 24420770
Local coupling of TRPC6 to ANO1/TMEM16A channels in smooth muscle cells amplifies vasoconstriction in cerebral arteries. PMID: 27147559
Ca2+-activated Cl- channel TMEM16A/ANO1 identified in zebrafish skeletal muscle is crucial for action potential acceleration. PMID: 30631052
ANO1 plays a critical role in prostatic hyperplasia. PMID: 26216998
Inhibition of Ca(2+)-activated Cl(-) channel ANO1/TMEM16A expression suppresses tumor growth and invasiveness in human prostate carcinoma. PMID: 22820160
A novel exon in the human Ca2+-activated Cl- channel Ano1 imparts greater sensitivity to intracellular Ca2. PMID: 26359375
DNA methylation regulates TMEM16A/ANO1 expression through multiple CpG islands in head and neck squamous cell carcinoma. PMID: 29123240
ANO1 as a marker of oral squamous cell carcinoma and silencing ANO1 suppresses migration of human SCC-25 cells. PMID: 24316695
Ano1/TMEM16A Overexpression Is Associated with Good Prognosis in PR-Positive or HER2-Negative Breast Cancer Patients following Tamoxifen Treatment. PMID: 25961581
ANO1 in urethral SMCs contributes to sex differences in urethral spontaneous tone. PMID: 32686521
miR-106a Targets Anoctamin 1 (ANO1) to Regulate Lipopolysaccharide (LPS)-Induced Inflammatory Response in Macrophages. PMID: 33037174
Inhibition of ANO1/TMEM16A Chloride Channel by Idebenone and Its Cytotoxicity to Cancer Cell Lines. PMID: 26196390
A negative Smad2/miR-9/ANO1 regulatory loop is responsible for LPS-induced sepsis. PMID: 31174089
Inhibition of ANO1 by Cis- and Trans-Resveratrol and Their Anticancer Activity in Human Prostate Cancer PC-3 Cells. PMID: 36674697
Effects of the Calcium-Activated Chloride Channel Inhibitors T16Ainh-A01 and CaCCinh-A01 on Cardiac Fibroblast Function. PMID: 30165368
Ano1, a Ca2+-activated Cl- channel, coordinates contractility in mouse intestine by Ca2+ transient coordination between interstitial cells of Cajal. PMID: 25063822
Local Ca2+ signals couple activation of TRPV1 and ANO1 sensory ion channels. PMID: 32345727
Targeting ion channels in cystic fibrosis. PMID: 26115565
For research use only. Not for human or drug use.
