Catalog No.
YHK90401
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Gly34-Thr89
Predicted molecular weight
34.21 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9HC07
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
TPARL, TMEM165, Transmembrane protein PT27, Transmembrane protein TPARL, Transmembrane protein 165
The human Golgi protein TMEM165 transports calcium and manganese in yeast and bacterial cells. PMID: 32047108
Novel role for the Golgi membrane protein TMEM165 in control of migration and invasion for breast carcinoma. PMID: 32733646
TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity. PMID: 30015898
Manganese-induced turnover of TMEM165. PMID: 28270545
TMEM165 deficiencies in Congenital Disorders of Glycosylation type II (CDG-II): Clues and evidences for roles of the protein in Golgi functions and ion homeostasis. PMID: 27401145
Congenital Disorders of N-Linked Glycosylation and Multiple Pathway Overview. PMID: 20301507
Dissection of TMEM165 function in Golgi glycosylation and its Mn2+ sensitivity. PMID: 31351090
Milk biosynthesis requires the Golgi cation exchanger TMEM165. PMID: 30622138
SPCA1 governs the stability of TMEM165 in Hailey-Hailey disease. PMID: 32335229
Investigating the functional link between TMEM165 and SPCA1. PMID: 31652305
Impact of disease-causing mutations on TMEM165 subcellular localization, a recently identified protein involved in CDG-II. PMID: 23575229
Glycosylation abnormalities in Gdt1p/TMEM165 deficient cells result from a defect in Golgi manganese homeostasis. PMID: 27008884
Evidence for splice transcript variants of TMEM165, a gene involved in CDG. PMID: 28088503
Insights into the regulation of cellular Mn2+ homeostasis via TMEM165. PMID: 37062452
Antisense-mediated therapeutic pseudoexon skipping in TMEM165-CDG. PMID: 24720419
TMEM165 deficiency causes a congenital disorder of glycosylation. PMID: 22683087
Fetal bovine serum impacts the observed N-glycosylation defects in TMEM165 KO HEK cells. PMID: 31415112
Towards understanding the extensive diversity of protein N-glycan structures in eukaryotes. PMID: 34873817
Involvement of thapsigargin- and cyclopiazonic acid-sensitive pumps in the rescue of TMEM165-associated glycosylation defects by Mn2. PMID: 30307768
TMEM165 Deficiency: Postnatal Changes in Glycosylation. PMID: 26238249
Abnormal cartilage development and altered N-glycosylation in Tmem165-deficient zebrafish mirrors the phenotypes associated with TMEM165-CDG. PMID: 25609749
TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy. PMID: 34930890
Differential Effects of D-Galactose Supplementation on Golgi Glycosylation Defects in TMEM165 Deficiency. PMID: 35693943
In Situ Fluorescent and Photoacoustic Imaging of Golgi pH to Elucidate the Function of Transmembrane Protein 165. PMID: 32003966
Galactose Supplementation in Patients With TMEM165-CDG Rescues the Glycosylation Defects. PMID: 28323990
The Ca(2+)/H(+) antiporter TMEM165 expression, localization in the developing, lactating and involuting mammary gland parallels the secretory pathway Ca(2+) ATPase (SPCA1). PMID: 24530912
COVID-19 Salivary Protein Profile: Unravelling Molecular Aspects of SARS-CoV-2 Infection. PMID: 36233441
From the Uncharacterized Protein Family 0016 to the GDT1 family: Molecular insights into a newly-characterized family of cation secondary transporters. PMID: 32743000
The Relationship between rs534654 Polymorphism in TMEM165 Gene and Increased Risk of Bipolar Disorder Type 1. PMID: 34703799
Newly characterized Golgi-localized family of proteins is involved in calcium and pH homeostasis in yeast and human cells. PMID: 23569283
CDG Therapies: From Bench to Bedside. PMID: 29702557
Bone Dysplasia as a Key Feature in Three Patients with a Novel Congenital Disorder of Glycosylation (CDG) Type II Due to a Deep Intronic Splice Mutation in TMEM165. PMID: 23430531
The yeast protein Gdt1p transports Mn2+ ions and thereby regulates manganese homeostasis in the Golgi. PMID: 29632074
Yeast Gdt1 is a Golgi-localized calcium transporter required for stress-induced calcium signaling and protein glycosylation. PMID: 27075443
Yeast as a Tool for Deeper Understanding of Human Manganese-Related Diseases. PMID: 31319631
Biometals and glycosylation in humans: Congenital disorders of glycosylation shed lights into the crucial role of Golgi manganese homeostasis. PMID: 32599014
Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation. PMID: 30481169
H+ and Pi Byproducts of Glycosylation Affect Ca2+ Homeostasis and Are Retrieved from the Golgi Complex by Homologs of TMEM165 and XPR1. PMID: 29042410
Skeletal and Bone Mineral Density Features, Genetic Profile in Congenital Disorders of Glycosylation: Review. PMID: 34441372
Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy. PMID: 33871553
ZIP14 is degraded in response to manganese exposure. PMID: 31541377
Plants contain small families of UPF0016 proteins including the PHOTOSYNTHESIS AFFECTED MUTANT71 transporter. PMID: 28075225
Transcriptomic Profiling of Ca2+ Transport Systems During the Formation of the Cerebral Cortex in Mice. PMID: 32751129
Serum bikunin isoforms in congenital disorders of glycosylation and linkeropathies. PMID: 32700771
Identification of candidate neoantigens produced by fusion transcripts in human osteosarcomas. PMID: 30674975
Variants in clock genes could be associated with lower risk of type 2 diabetes in an elderly Greek population. PMID: 34674804
Acidic and uncharged polar residues in the consensus motifs of the yeast Ca2+ transporter Gdt1p are required for calcium transport. PMID: 28114750
CaGdt1 plays a compensatory role for the calcium pump CaPmr1 in the regulation of calcium signaling and cell wall integrity signaling in Candida albicans. PMID: 29954393
Whole-Transcriptome Analysis of Serum L1CAM-Captured Extracellular Vesicles Reveals Neural and Glycosylation Changes in Autism Spectrum Disorder. PMID: 35412111
Novel Metal Cation Resistance Systems from Mutant Fitness Analysis of Denitrifying Pseudomonas stutzeri. PMID: 27474723
Molecular evolution of a novel family of putative calcium transporters. PMID: 24955841
Gene Replacement in Arabidopsis Reveals Manganese Transport as an Ancient Feature of Human, Plant and Cyanobacterial UPF0016 Proteins. PMID: 34194462
Serum N-glycan and O-glycan analysis by mass spectrometry for diagnosis of congenital disorders of glycosylation. PMID: 23928051
For research use only. Not for human or drug use.
