Overview
Catalog No.
PHJ62601
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human MMP25 (Tyr108-Ile313).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Membrane-type matrix metalloproteinase 6, MMP-25, MT6MMP, MMPL1, MT-MMP 6, MTMMP6, Membrane-type-6 matrix metalloproteinase, MT6-MMP, Matrix metalloproteinase-25, Leukolysin, MMP25, MMP20
Purification
Purified by antigen affinity column.
Accession
Q9NPA2
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Data Image
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Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with MMP25 antibody (PHJ62601) at 1 μg/ml.
Lane 1: Recombinant protein
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 25 kDa
Observed MW: 25 kDa
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Various lysates were subjected to SDS PAGE followed by western blot with MMP25 antibody (PHJ62601) at 1 μg/ml.
Lane 1: Hela
Lane 2: MCF7
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 62 kDa
Observed MW: 62 kDa
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Immunohistochemical analysis of Human lung cancer tissue stained for MMP25 with PHJ62601.
References
MMP2 is a immunotherapy related biomarker and correlated with cancer-associated fibroblasts infiltrate in melanoma., PMID:36788565
Serum biomarkers in elderly asthma., PMID:23952480
Membrane-type 6 matrix metalloproteinase regulates the activation-induced downmodulation of CD16 in human primary NK cells., PMID:23851692
Genome-wide identification of possible methylation markers chemosensitive to targeted regimens in colorectal cancers., PMID:21850381
Matrix metalloproteinase-25 has a functional role in mouse secondary palate development and is a downstream target of TGF-β3., PMID:20809987
Serum metalloproteinase leukolysin (MMP-25/MT-6): a potential metabolic marker for atopy-associated inflammation., PMID:20337648
Gingival crevicular fluid matrix metalloproteinase-25 and -26 levels in periodontal disease., PMID:16584348
Catalytic activities of membrane-type 6 matrix metalloproteinase (MMP25)., PMID:11226436
Datasheet