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Anti-AXIN1 Polyclonal Antibody

Catalog #:   PHA43901 Specific References (27) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: O15169
Overview

Catalog No.

PHA43901

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human AXIN1 (Ser653-Asp862).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

AXIN, Axin-1, hAxin, Axis inhibition protein 1, AXIN1

Purification

Purified by antigen affinity column.

Accession

O15169

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with AXIN1 antibody (PHA43901) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 26 kDa
    Observed MW: 26 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with AXIN1 antibody (PHA43901) at 1 μg/ml.

    Lane 1: SW480

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 95 kDa
    Observed MW: 95 kDa
  • Immunohistochemical
    Immunohistochemical analysis of Human Breast stained for AXIN1 with PHA43901.
References

Precision targeting of β-catenin induces tumor reprogramming and immunity in hepatocellular cancers., PMID:40442146

A novel drug prejudice scaffold-imidazopyridine-conjugate can promote cell death in a colorectal cancer model by binding to β-catenin and suppressing the Wnt signaling pathway., PMID:39067696

Inflammatory related plasma proteins involved in acute preschool wheeze., PMID:38006384

Nogo-B deficiency suppresses white adipogenesis by regulating β-catenin signaling., PMID:36931495

TSH receptor antibodies (TRAb) - A potential new biomarker for endometriosis., PMID:36152376

Mechanisms of miR‑128‑3p in inhibiting osteoblast differentiation from bone marrow‑derived mesenchymal stromal cells., PMID:33174052

Genomic Analysis of Germline Variation Associated with Survival of Patients with Colorectal Cancer Treated with Chemotherapy Plus Biologics in CALGB/SWOG 80405 (Alliance)., PMID:32958699

The Immunome of Colon Cancer: Functional In Silico Analysis of Antigenic Proteins Deduced from IgG Microarray Profiling., PMID:29505855

Long noncoding RNA papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) inhibits proliferation and invasion of glioma cells by suppressing the Wnt/β-catenin signaling pathway., PMID:28187755

Aptamer-Targeted Attenuation of IL-2 Signaling in CD8+ T Cells Enhances Antitumor Immunity., PMID:28129128

The destruction complex of beta-catenin in colorectal carcinoma and colonic adenoma., PMID:27462886

Protein Profile and Morphological Alterations in Penumbra after Focal Photothrombotic Infarction in the Rat Cerebral Cortex., PMID:27324898

The prostate cancer immunome: In silico functional analysis of antigenic proteins from microarray profiling with IgG., PMID:27089054

Tissue inhibitor of metalloproteinase 2 inhibits activation of the β-catenin signaling in melanoma cells., PMID:25839957

The increase in maternal expression of axin1 and axin2 contribute to the zebrafish mutant ichabod ventralized phenotype., PMID:25335865

Human Sarcoma growth is sensitive to small-molecule mediated AXIN stabilization., PMID:24842792

A quantitative trait locus for a primary antibody response to keyhole limpet hemocyanin on chicken chromosome 14--confirmation and candidate gene approach., PMID:20709969

The Wnt/beta-catenin pathway regulates growth and maintenance of colonospheres., PMID:20691072

Epigenetic inactivation of SFRP genes in oral squamous cell carcinoma., PMID:18497987

Concomitant activation of Wnt pathway and loss of mismatch repair function in human melanoma., PMID:18384130

Wnt signal amplification via activity, cooperativity, and regulation of multiple intracellular PPPSP motifs in the Wnt co-receptor LRP6., PMID:18362152

WNT signaling pathway and stem cell signaling network., PMID:17634527

Identification of SP5 as a downstream gene of the beta-catenin/Tcf pathway and its enhanced expression in human colon cancer., PMID:16273202

Mutations of the APC, beta-catenin, and axin 1 genes and cytoplasmic accumulation of beta-catenin in oral squamous cell carcinoma., PMID:16163548

Infrequent mutation of APC, AXIN1, and GSK3B in human pituitary adenomas with abnormal accumulation of CTNNB1., PMID:15981102

Domains of axin and disheveled required for interaction and function in wnt signaling., PMID:11027605

Membrane-anchored plakoglobins have multiple mechanisms of action in Wnt signaling., PMID:10512857

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-AXIN1 Polyclonal Antibody [PHA43901]
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