Catalog No.
PXX01001
Species reactivity
Clostridium perfringens
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Clostridium perfringens plc/Phospholipase C/Alpha-toxin (Ala28-Lys398).
Tested applications
ELISA: 1:2000-1:8000, IHC: 1:50-1:200, WB: 1:500-1:4000
Target
Phospholipase C, PLC, 3.1.4.3, Alpha-toxin, Hemolysin, Lecithinase, Phosphatidylcholine cholinephosphohydrolase, plc, cpa, CPE0036
Concentration
1.15 mg/ml
Purification
Purified by antigen affinity column.
Accession
P0C216
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Generation of recombinant baculovirus expressing atoxic C-terminal CPA toxin of Clostridium perfringens and production of specific antibodies., PMID:31992276
Immunization of rabbits with recombinant Clostridium perfringens alpha toxins CPA-C and CTB-CPA-C in a bicistronic design expression system confers strong protection against challenge., PMID:31811913
Immunization with a nontoxic naturally occurring Clostridium perfringens alpha toxin induces neutralizing antibodies in rabbits., PMID:29246841
A Fast and Inexpensive Protocol for Empirical Verification of Neutralizing Epitopes in Microbial Toxins and Enzymes., PMID:28660200
A chimeric protein composed of the binding domains of Clostridium perfringens phospholipase C and Trueperella pyogenes pyolysin induces partial immunoprotection in a mouse model., PMID:27473983
Defining the optimal formulation and schedule of a candidate toxoid vaccine against Clostridium difficile infection: A randomized Phase 2 clinical trial., PMID:27013431
Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity., PMID:24631214
A recombinant carboxy-terminal domain of alpha-toxin protects mice against Clostridium perfringens., PMID:23668605
Release of glycoprotein (GP1) from the tegumental surface of Taenia solium by phospholipase C from Clostridium perfringens suggests a novel protein-anchor to membranes., PMID:20130782
Recombinant attenuated Salmonella enterica serovar typhimurium expressing the carboxy-terminal domain of alpha toxin from Clostridium perfringens induces protective responses against necrotic enteritis in chickens., PMID:18337376
Dissecting the contributions of Clostridium perfringens type C toxins to lethality in the mouse intravenous injection model., PMID:16926413
Immunization with an alphatoxin variant 121A/91-R212H protects mice against Clostridium perfringens alphatoxin., PMID:16701610
Signal transduction mechanism involved in Clostridium perfringens alpha-toxin-induced superoxide anion generation in rabbit neutrophils., PMID:16622226
Effects of lactulose on the intestinal microflora of periparturient sows and their piglets., PMID:12420113
A field study of naturally occurring specific antibodies against Clostridium perfringens alpha toxin in Norwegian broiler flocks., PMID:11569751
HC fragment (C-terminal portion of the heavy chain) of tetanus toxin activates protein kinase C isoforms and phosphoproteins involved in signal transduction., PMID:11336640
Identification of residues critical for toxicity in Clostridium perfringens phospholipase C, the key toxin in gas gangrene., PMID:10931204
Involvement of RhoA and its interaction with protein kinase C and Src in CCK-stimulated pancreatic acini., PMID:10198335
Detection of Clostridium perfringens alpha toxin using a capture antibody ELISA., PMID:10080352
UDP-glucose deficiency causes hypersensitivity to the cytotoxic effect of Clostridium perfringens phospholipase C., PMID:9733734
A recombinant, membrane-acting immunotoxin., PMID:1898711
Monoclonal antibodies against alpha toxin of Clostridium perfringens., PMID:2544482
[Isolation and purification of phospholipase C from Clostridium perfringens and antibodies against it using polymeric biospecific adsorbents]., PMID:2902618
On the in vitro neutralization test of Clostridium perfringens type A toxin., PMID:2900852
Improved rubella hemagglutination inhibtion test: inactivation of non-immunoglobulin hemagglutination inhibitors by phospholipase C., PMID:187617
For research use only. Not for human or drug use.
