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Anti-AP1S2 Polyclonal Antibody (PHF16701)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:P56377
Overview

Catalog No.

PHF16701

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human AP1S2 (Met1-Thr157).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Sigma1B-adaptin, Sigma 1B subunit of AP-1 clathrin, AP1S2, Adaptor-related protein complex 1 subunit sigma-1B, Sigma-adaptin 1B, AP-1 complex subunit sigma-2, Clathrin assembly protein complex 1 sigma-1B small chain, Adaptor protein complex AP-1 subunit sigma-1B, Golgi adaptor HA1/AP1 adaptin sigma-1B subunit

Concentration

1.34 mg/ml

Purification

Purified by antigen affinity column.

Accession

P56377

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Various lysates were subjected to SDS PAGE followed by western blot with AP1S2 antibody (PHF16701) at 1μg/mL .

    Lane 1: Raji cell lysate
    Lane 2: Mouse brain lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 19 kDa
    Observed MW: 20 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with AP1S2 antibody (PHF16701) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 21 kDa
    Observed MW: 21 kDa
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-AP1S2 Polyclonal Antibody [PHF16701]
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