Catalog No.
PHA09001
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human CDC7 (Met1-Thr232).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
HsCdc7, CDC7L1, Cell division cycle 7-related protein kinase, CDC7-related kinase, huCdc7, CDC7
Purification
Purified by antigen affinity column.
Accession
O00311
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Profiling of Signaling Proteins in Penumbra After Focal Photothrombotic Infarct in the Rat Brain Cortex., PMID:27771897
CHO cell culture longevity and recombinant protein yield are enhanced by depletion of miR-7 activity via sponge decoy vectors., PMID:24166820
Preparation and identification of a novel antibody against human CDC7 kinase., PMID:24111867
Effects of CDC7 gene silencing and Rituximab on apoptosis in diffuse large B cell lymphoma cells., PMID:22806309
Molecular architecture of the DNA replication origin activation checkpoint., PMID:20729811
American Association for Cancer Research--99th Annual Meeting. Clinical and preclinical data on promising therapeutics., PMID:18509781
Inhibition of Cdc7/Dbf4 kinase activity affects specific phosphorylation sites on MCM2 in cancer cells., PMID:18286467
Phosphorylation of MCM4 by Cdc7 kinase facilitates its interaction with Cdc45 on the chromatin., PMID:17046832
Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells., PMID:16899510
Importin-beta mediates Cdc7 nuclear import by binding to the kinase insert II domain, which can be antagonized by importin-alpha., PMID:16492669
Identification of Mcm2 phosphorylation sites by S-phase-regulating kinases., PMID:16446360
ATR and ATM regulate the timing of DNA replication origin firing., PMID:15220931
A generic time-resolved fluorescence assay for serine/threonine kinase activity: application to Cdc7/Dbf4., PMID:12895303
A 63-base pair DNA segment containing an Sp1 site but not a canonical E2F site can confer growth-dependent and E2F-mediated transcriptional stimulation of the human ASK gene encoding the regulatory subunit for human Cdc7-related kinase., PMID:12015319
Byr4 localizes to spindle-pole bodies in a cell cycle-regulated manner to control Cdc7 localization and septation in fission yeast., PMID:10799520
The role of the sid1p kinase and cdc14p in regulating the onset of cytokinesis in fission yeast., PMID:10775265
Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication., PMID:10523313
Asymmetry of the spindle pole bodies and spg1p GAP segregation during mitosis in fission yeast., PMID:10381387
A novel growth- and cell cycle-regulated protein, ASK, activates human Cdc7-related kinase and is essential for G1/S transition in mammalian cells., PMID:10373557
DNA replication in vertebrates requires a homolog of the Cdc7 protein kinase., PMID:10077591
Oligomers of the Cdc7/Dbf4 protein kinase exist in the yeast cell., PMID:9790600
Asymmetric segregation on spindle poles of the Schizosaccharomyces pombe septum-inducing protein kinase Cdc7p., PMID:9420333
Reference sera for antinuclear antibodies. II. Further definition of antibody specificities in international antinuclear antibody reference sera by immunofluorescence and western blotting., PMID:9082926
Preparation of active Cdc7/Dbf4 kinase from yeast cells., PMID:9251036
A 48-kDa, S-antigen-like phosphoprotein in yeast DNA-replicative complex preparations., PMID:1860866
The CDC7 protein of Saccharomyces cerevisiae is a phosphoprotein that contains protein kinase activity., PMID:2023904
DNA metabolism gene CDC7 from yeast encodes a serine (threonine) protein kinase., PMID:2166954
For research use only. Not for human or drug use.
