Catalog No.
PHD87701
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human CDC27 (Asn17-Ser231).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
ANAPC3, Cell division cycle protein 27 homolog, CDC27, APC3, Anaphase-promoting complex subunit 3, CDC27Hs, D0S1430E, CDC27 homolog, D17S978E, H-NUC
Purification
Purified by antigen affinity column.
Accession
P30260
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Proteomic and serological markers for diagnosing cardia gastric cancer and precursor lesions in a Chinese population., PMID:39455684
The Drosophila Bub3 protein is required for the mitotic checkpoint and for normal accumulation of cyclins during G2 and early stages of mitosis., PMID:15615783
Mitotic regulation of the human anaphase-promoting complex by phosphorylation., PMID:14657031
The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis., PMID:12429948
The APC is dispensable for first meiotic anaphase in Xenopus oocytes., PMID:11146630
Isolation and identification of the third subunit of mammalian DNA polymerase delta by PCNA-affinity chromatography of mouse FM3A cell extracts., PMID:10219083
Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events., PMID:9628895
The essential mitotic peptidyl-prolyl isomerase Pin1 binds and regulates mitosis-specific phosphoproteins., PMID:9499405
TPR proteins required for anaphase progression mediate ubiquitination of mitotic B-type cyclins in yeast., PMID:8744951
A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B., PMID:7736580
CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition., PMID:7736578
Cdc16p, Cdc23p and Cdc27p form a complex essential for mitosis., PMID:7925276
BIMA, a TPR-containing protein required for mitosis, localizes to the spindle pole body in Aspergillus nidulans., PMID:8432735
For research use only. Not for human or drug use.
