Anti-NPC1 Polyclonal Antibody

Catalog No.

PHA41901

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NPC1 (Gln23-Asp266).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

NPC intracellular cholesterol transporter 1, Niemann-Pick C1 protein, NPC1

Purification

Purified by antigen affinity column.

Accession

O15118

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Development of a neutralization assay and bioluminescent imaging mouse model for Dehong virus (DEHV) using a pseudovirus system., PMID:40172216

Prevalence of Neutralizing Antibodies to AAV2 and AAV9 in Individuals with Niemann-Pick Disease, Type C1., PMID:40014475

Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection., PMID:39173055

A novel MARV glycoprotein-specific antibody with potentials of broad-spectrum neutralization to filovirus., PMID:38526940

Cdc42-driven endosomal cholesterol transport promotes collateral resistance in HER2-positive gastric cancer., PMID:38336288

A Pan-Ebolavirus Monoclonal Antibody Cocktail Provides Protection Against Ebola and Sudan Viruses., PMID:37288609

Traits, phylogeny and host cell receptors predict Ebolavirus host status among African mammals., PMID:36542657

Ligand-based design of peptide entry inhibitors targeting the endosomal receptor binding site of filoviruses., PMID:36007601

Case Report: Be Aware of "New" Features of Niemann-Pick Disease: Insights From Two Pediatric Cases., PMID:35360843

Comparative analyses of small molecule and antibody inhibition on glycoprotein-mediated entry of Měnglà virus with other filoviruses., PMID:35332563

Pharmacologically induced endolysosomal cholesterol imbalance through clinically licensed drugs itraconazole and fluoxetine impairs Ebola virus infection in vitro., PMID:34919035

The CD22-IGF2R interaction is a therapeutic target for microglial lysosome dysfunction in Niemann-Pick type C., PMID:34851695

Two Distinct Lysosomal Targeting Strategies Afford Trojan Horse Antibodies With Pan-Filovirus Activity., PMID:34721393

Understanding the Clinical Impact of MUC5AC Expression on Pancreatic Ductal Adenocarcinoma., PMID:34205412

Potential pharmacological strategies targeting the Niemann-Pick C1 receptor and Ebola virus glycoprotein interaction., PMID:34175537

Direct Intracellular Visualization of Ebola Virus-Receptor Interaction by In Situ Proximity Ligation., PMID:33436438

Plasmid DNA gene therapy of the Niemann-Pick C1 mouse with transferrin receptor-targeted Trojan horse liposomes., PMID:32770132

Niemann-Pick type C1 regulates cholesterol transport and metamorphosis in silkworm, Bombyx mori (Dazao)., PMID:32112844

Real-Time Analysis of Individual Ebola Virus Glycoproteins Reveals Pre-Fusion, Entry-Relevant Conformational Dynamics., PMID:31952255

Baculovirus Utilizes Cholesterol Transporter NIEMANN-Pick C1 for Host Cell Entry., PMID:31866985

Niemann-Pick disease type C presenting as very early onset inflammatory bowel disease., PMID:31352388

Pneumococcal Immunization Reduces Neurological and Hepatic Symptoms in a Mouse Model for Niemann-Pick Type C1 Disease., PMID:30666257

Astemizole Inhibits mTOR Signaling and Angiogenesis by Blocking Cholesterol Trafficking., PMID:30123067

The Marburgvirus-Neutralizing Human Monoclonal Antibody MR191 Targets a Conserved Site to Block Virus Receptor Binding., PMID:29324225

Intra-endosomal trafficking mediated by lysobisphosphatidic acid contributes to intracellular release of phosphorothioate-modified antisense oligonucleotides., PMID:28379543

Investigational therapies for hypercholesterolemia., PMID:28368771

Primary cilium alterations and expression changes of Patched1 proteins in niemann-pick type C disease., PMID:28332184

Telocytes: a potential defender in the spleen of Npc1 mutant mice., PMID:27860245

Prevention of oxLDL uptake leads to decreased atherosclerosis in hematopoietic NPC1-deficient Ldlr-/- mice., PMID:27816810

The Tetherin Antagonism of the Ebola Virus Glycoprotein Requires an Intact Receptor-Binding Domain and Can Be Blocked by GP1-Specific Antibodies., PMID:27707924

Cathepsin S contributes to microglia-mediated olfactory dysfunction through the regulation of Cx3cl1-Cx3cr1 axis in a Niemann-Pick disease type C1 model., PMID:27687148

A "Trojan horse" bispecific-antibody strategy for broad protection against ebolaviruses., PMID:27608667

Positive Selection Drives Evolution at the Host-Filovirus Interaction Surface., PMID:27512112

A phase 1 dose-escalation study of NEO-102 in patients with refractory colon and pancreatic cancer., PMID:27449137

Mapping of Ebolavirus Neutralization by Monoclonal Antibodies in the ZMapp Cocktail Using Cryo-Electron Tomography and Studies of Cellular Entry., PMID:27279622

Potent neutralizing monoclonal antibodies against Ebola virus infection., PMID:27181584

Chasing Ebola through the Endosomal Labyrinth., PMID:27006455

Host-Primed Ebola Virus GP Exposes a Hydrophobic NPC1 Receptor-Binding Pocket, Revealing a Target for Broadly Neutralizing Antibodies., PMID:26908579

Direct Visualization of Ebola Virus Fusion Triggering in the Endocytic Pathway., PMID:26861015

Niemann-Pick disease type C1(NPC1) is involved in resistance against imatinib in the imatinib-resistant Ph+ acute lymphoblastic leukemia cell line SUP-B15/RI., PMID:26818574

Circadian profiling in two mouse models of lysosomal storage disorders; Niemann Pick type-C and Sandhoff disease., PMID:26467605

BK Channels Alleviate Lysosomal Storage Diseases by Providing Positive Feedback Regulation of Lysosomal Ca2+ Release., PMID:25982675

Interaction between TIM-1 and NPC1 Is Important for Cellular Entry of Ebola Virus., PMID:25855742

Structural basis for Marburg virus neutralization by a cross-reactive human antibody., PMID:25723165

Intracisternal cyclodextrin prevents cerebellar dysfunction and Purkinje cell death in feline Niemann-Pick type C1 disease., PMID:25717099

Cell entry by a novel European filovirus requires host endosomal cysteine proteases and Niemann-Pick C1., PMID:25310500

Visualization of cholesterol deposits in lysosomes of Niemann-Pick type C fibroblasts using recombinant perfringolysin O., PMID:24775609

Analysis of Lujo virus cell entry using pseudotype vesicular stomatitis virus., PMID:24741091

Comprehensive functional analysis of N-linked glycans on Ebola virus GP1., PMID:24473128

Olfactory deficits in Niemann-Pick type C1 (NPC1) disease., PMID:24391715

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