Catalog No.
PHA56101
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human CYP27B1 (Asp256-Arg508).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
25-hydroxyvitamin D(3) 1-alpha-hydroxylase, CYP27B, CYP27B1, Cytochrome P450C1 alpha, Calcidiol 1-monooxygenase, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, CYP1ALPHA, Cytochrome P450 subfamily XXVIIB polypeptide 1, 25-OHD-1 alpha-hydroxylase, VD3 1A hydroxylase, 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Purification
Purified by antigen affinity column.
Accession
O15528
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
In ovo administration of the Marek's disease vaccine in conjunction with 25-hydroxyvitamin D3 and its subsequent effects on the performance and immunity-related characteristics of Ross 708 broiler hatchlings1,2,3., PMID:37939590
Expression of Rat Cyp27b1 in HepG2 Cells Using Adenovirus Vector and Its Application to Evaluation of Self-Made and Commercially Available Anti-Cyp27b1 Antibodies., PMID:37121728
Hypercalcemia Associated with the Ectopic Expression of 25-hydroxyvitamin D3-1α-hydroxylase in Diffuse Large B-cell Lymphoma., PMID:35965075
Anti-Thymocyte Globulin Treatment Augments 1,25-Dihydroxyvitamin D3 Serum Levels in Patients Undergoing Hematopoietic Stem Cell Transplantation., PMID:35058935
Vitamin D-Related Genetics as Predictive Biomarker of Clinical Remission in Adalimumab-Treated Patients Affected by Crohn's Disease: A Pilot Study., PMID:34959633
Pollutants enhance IgE sensitization in the gut via local alteration of vitamin D-metabolizing enzymes., PMID:34504311
Vitamin D upregulates the macrophage complement receptor immunoglobulin in innate immunity to microbial pathogens., PMID:33767430
Impaired Vitamin D Signaling Is Associated With Frequent Development of Renal Cell Tumor in End-stage Kidney Disease., PMID:33109593
Placental gene expression and antibody levels of mother-neonate pairs reveal an enhanced risk for inflammation in a helminth endemic country., PMID:31673046
Vitamin D Is Required for ILC3 Derived IL-22 and Protection From Citrobacter rodentium Infection., PMID:30723466
Lenalidomide enhances MOR202-dependent macrophage-mediated effector functions via the vitamin D pathway., PMID:29654274
Vitamin D in Graves Disease: Levels, Correlation with Laboratory and Clinical Parameters, and Genetics., PMID:29594051
High-dose vitamin D in Addison's disease regulates T-cells and monocytes: A pilot trial., PMID:29522979
FGF23 Neutralizing Antibody Partially Improves Bone Mineralization Defect of HMWFGF2 Isoforms in Transgenic Female Mice., PMID:29502359
A Polymorphism within the Vitamin D Transporter Gene Predicts Outcome in Metastatic Colorectal Cancer Patients Treated with FOLFIRI/Bevacizumab or FOLFIRI/Cetuximab., PMID:29208668
Endogenous Calcitriol Synthesis Controls the Humoral IgE Response in Mice., PMID:29109124
Down-regulation of CYP27B1 gene expression in Iranian patients with relapsing-remitting multiple sclerosis., PMID:27792005
Comparable vitamin D3 metabolism in the endometrium of patients with recurrent spontaneous abortion and fertile controls., PMID:25914243
The vitamin D receptor (VDR) is expressed in skeletal muscle of male mice and modulates 25-hydroxyvitamin D (25OHD) uptake in myofibers., PMID:24949660
Vitamin D effects on B cell function in autoimmunity., PMID:24761763
Elevated fibroblast growth factor 23 exerts its effects on placenta and regulates vitamin D metabolism in pregnancy of Hyp mice., PMID:24470103
Mechanisms of vitamin D₃ metabolite repression of IgE-dependent mast cell activation., PMID:24461581
Uric acid suppresses 1 alpha hydroxylase in vitro and in vivo., PMID:24269076
Tumor-produced versican V1 enhances hCAP18/LL-37 expression in macrophages through activation of TLR2 and vitamin D3 signaling to promote ovarian cancer progression in vitro., PMID:23424670
Distribution of vitamin D receptor and 1α-hydroxylase in male mouse reproductive tract., PMID:23188491
Confirmation of the genetic association of CTLA4 and PTPN22 with ANCA-associated vasculitis., PMID:19951419
Understanding and determining the etiology of autism., PMID:19774457
Calcitonin, a regulator of the 25-hydroxyvitamin D3 1alpha-hydroxylase gene., PMID:19261615
Serum 25-hydroxyvitamin D and IgE - a significant but nonlinear relationship., PMID:19154546
Vitamin D deficiency modulates Graves' hyperthyroidism induced in BALB/c mice by thyrotropin receptor immunization., PMID:18927213
Expression of 25-hydroxyvitamin D3-1alpha-hydroxylase in subcutaneous fat necrosis., PMID:18811689
Calcitriol affects hCG gene transcription in cultured human syncytiotrophoblasts., PMID:18211694
25-Hydroxyvitamin D3 1alpha-hydroxylase splice variants in breast cell lines MCF-7 and MCF-10., PMID:17878529
PLXDC1 (TEM7) is identified in a genome-wide expression screen of glioblastoma endothelium., PMID:17031559
A comparison of gene expression in mouse liver and kidney in obstructive cholestasis utilizing high-density oligonucleotide microarray technology., PMID:16688799
Vitamin D(3) metabolism in human glioblastoma multiforme: functionality of CYP27B1 splice variants, metabolism of calcidiol, and effect of calcitriol., PMID:16061850
25-hydroxyvitamin D3-1alpha-hydroxylase expression in normal and malignant human colon., PMID:15208365
Vitamin D autocrine system and prostate cancer., PMID:12899525
Regulation of the 25-hydroxyvitamin D-1alpha-hydroxylase gene and its splice variant., PMID:12899521
Conflicting actions of parathyroid hormone-related protein and serum calcium as regulators of 25-hydroxyvitamin D(3)-1 alpha-hydroxylase expression in a nude rat model of humoral hypercalcemia of malignancy., PMID:11691644
A possible role for CYP27 as a major renal mitochondrial 25-hydroxyvitamin D3 1 alpha-hydroxylase., PMID:8706816
Regulators of chondrocyte differentiation in tibial dyschondroplasia: an in vivo and in vitro study., PMID:8541142
The vitamin D3 hydroxylase-associated protein is a propionamide-metabolizing amidase enzyme., PMID:7840608
Liver mitochondrial cytochrome P450 CYP27 and recombinant-expressed human CYP27 catalyze 1 alpha-hydroxylation of 25-hydroxyvitamin D3., PMID:7937829
Constitutive synthesis of 1,25-dihydroxyvitamin D3 by a human small cell lung cancer cell line., PMID:8045976
cDNA cloning and characterization of a vitamin D3 hydroxylase-associated protein., PMID:8276793
The phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate stimulates the dephosphorylation of mitochondrial ferredoxin in cultured chick kidney cells., PMID:8404625
Immunopurified 25-hydroxyvitamin D 1 alpha-hydroxylase and 1,25-dihydroxyvitamin D 24-hydroxylase are closely related but distinct enzymes., PMID:1310688
Amino-terminal sequence homology of two chick kidney mitochondrial proteins immunoisolated with monoclonal antibodies to the cytochrome P450 of 25-hydroxyvitamin D3-1 alpha-hydroxylase., PMID:2028838
Tumor necrosis factor-alpha induces vitamin D-1-hydroxylase activity in normal human alveolar macrophages., PMID:1690216
For research use only. Not for human or drug use.
